Antibodies were quantified using IgM and IgG quantitation sets (Bethyl Laboratories Inc) according to the manufacturer’s protocol with some adjustments: Thermo Scientific Nunc MaxiSorp 96-well ELISA plates were coated with 50 µg/mL calf thymus DNA (Thermo Fisher Scientific), phosphorylcholine (Sigma-Aldrich), hen egg lysozyme (Sigma Aldrich), glomerulus extract isolated from primary mouse glomeruli lysed in NP40 lysis buffer, or anti-C3 (clone K13/16, BioLegend for immune complex determination) in sodium carbonate coating buffer. Sera was diluted at 1:50 for quantification. Signal was quantified against IgG/IgM standard and reported as absorbance units (AU).
Igm and igg quantitation sets
Manufactured by Fortis Life Sciences
The IgM and IgG quantitation sets are laboratory equipment used to quantify the levels of immunoglobulin M (IgM) and immunoglobulin G (IgG) in biological samples. These sets provide the necessary reagents and protocols to perform the quantitative analysis of these specific antibody classes.
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2 protocols using igm and igg quantitation sets
1
Quantification of Antibody Isotypes
2
Quantification of Antibody Isotypes
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Antibodies were quantified using IgM and IgG quantitation sets (Bethyl Laboratories Inc. ) according to the manufacturer's protocol with some adjustments : Thermo Scientific Nunc MaxiSorp 96 well ELISA plates were coated with 50 µg/mL Calf thymus DNA (Thermo Fisher Scientific), Phosphorylcholine (Sigma Aldrich), Hen Egg Lysozyme (Sigma Aldrich), Glomerulus Extract isolated from primary mouse glomeruli lysed in NP40 lysis buffer, or anti-C3 (clone K13/16, BioLegend for immune complex determination) in sodium carbonate coating buffer. Sera was diluted at 1:50 for quantification. Signal was quantified against IgG/IgM standard and reported as absorbance units (AU).
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