Sulforaphane (>95% pure) was purchased from Pioneer Herb Industrial Co. Ltd. (Shanghai, China). For the antibodies, mouse monoclonal anti-cleaved PARP, rabbit monoclonal anti-cleaved caspase-3, and rabbit monoclonal anti-γ-H2AX monoclonal antibodies (7631) were purchased from Cell Signaling Technology (CST, Danvers, MA, USA). Rabbit monoclonal anti-Rad51 antibodies were purchased from Abcam Biotechnology (Danvers, MA, USA). Anti-LATS2 was from Proteintech (Ag28221). Anti-MDC1 antibody was from Abcam (Danvers, MA, USA, ab241048). Anti-Tubulin antibody, and CoraLite 594-conjugated goat anti-rabbit IgG secondary antibody were purchased from Proteintech (10694).
Anti lats2
Manufactured by Proteintech
Sourced in United States
Anti-LATS2 is a protein-specific antibody that recognizes the Large Tumor Suppressor Kinase 2 (LATS2) protein. LATS2 is a serine/threonine-protein kinase that plays a role in the Hippo signaling pathway, which is important for regulating cell proliferation and apoptosis.
Automatically generated - may contain errors
Lab products found in correlation
Anti mdc1 antibody, by Abcam (1 mentions)
Rabbit monoclonal anti cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Anti tubulin antibody, by Proteintech (1 mentions)
Rabbit monoclonal anti vimentin, by Cell Signaling Technology (1 mentions)
Anti mmp9, by Proteintech (1 mentions)
Rabbit monoclonal anti e cadherin, by Cell Signaling Technology (1 mentions)
Anti β actin, by Merck Group (1 mentions)
Enhanced chemiluminescence detection kit, by Merck Group (1 mentions)
Anti α tubulin antibody, by Proteintech (1 mentions)
Anti lats1, by Proteintech (1 mentions)
Fluorchem fc2 multi imager 2, by Bio-Techne (1 mentions)
Anti β catenin, by Proteintech (1 mentions)
Anti aldh1a1, by Abcam (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
Protease inhibitor cocktail, by Roche (1 mentions)
Anti gapdh antibody, by Proteintech (1 mentions)
3 protocols using anti lats2
1
Sulforaphane-Induced DNA Damage Response
2
Protein Expression Analysis via Western Blot
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Western blot analysis was conducted as previously described [23 (link)]. The primary antibodies were used as follows: rabbit polyclonal anti-LATS2 (1 : 500, Proteintech, Rosemont, USA), rabbit polyclonal anti-YAP (1 : 500, Proteintech), rabbit monoclonal anti-phospho-YAP (Ser127, 1 : 1000, Cell Signaling Technology, Danvers, MA, USA), rabbit monoclonal anti-E-cadherin (1 : 1000, Cell Signaling Technology), rabbit monoclonal anti-Vimentin (1 : 1000, Cell Signaling Technology), and rabbit polyclonal anti-MMP9 (1 : 500, Proteintech). Mouse monoclonal anti-β-actin (1 : 1000, Sigma-Aldrich) was used as a reference.
3
Western Blot Analysis of Signaling Proteins
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Cells were lysed in ice-cold RIPA buffer containing a protease-inhibitor cocktail (Roche). Protein content was quanti ed with a BCA protein assay kit (Thermo Fisher Scienti c). About 30 μg of protein was subjected to electrophoresis, transferred onto PVDF membranes (Millipore), and blocked with 5% nonfat dry milk in Tris-buffered saline containing 0.1% Tween 20. Membranes were incubated overnight with the following primary antibodies: anti-AKT (dilution 1:1000; CST), anti-AKT1 (1:1000; CST), anti-p-AKT(Ser473) (1:1000; CST), anti-p-AKT (Thr308) (1:1000; CST), anti-β-catenin (1:2000; Proteintech), anti-ALDH1A1 (1:1000; Abcam), anti-Vimentin (1:500; CST), anti-MST1 (1:1000; Proteintech), anti-MST2 (1:1000; Proteintech), anti-LATS1 (1:1000; Proteintech), anti-LATS2 (1:1000; Proteintech), anti-p-LATS1/2
(1:1000; Absin), anti-YAP (1:1000; Ptoteintech), anti-p-YAP (1:1000; Absin). Anti-GAPDH antibody (1:3000; Proteintech) and anti-α-tubulin antibody (1:3000; Proteintech) served as endogenous controls. The speci c bands were visualized using secondary anti-rabbit or anti-mouse antibody (1:3000; Proteintech), enhanced chemiluminescence detection kit (Millipore), and FluorChem FC2 Multi-Imager II (Alpha Innotech).
(1:1000; Absin), anti-YAP (1:1000; Ptoteintech), anti-p-YAP (1:1000; Absin). Anti-GAPDH antibody (1:3000; Proteintech) and anti-α-tubulin antibody (1:3000; Proteintech) served as endogenous controls. The speci c bands were visualized using secondary anti-rabbit or anti-mouse antibody (1:3000; Proteintech), enhanced chemiluminescence detection kit (Millipore), and FluorChem FC2 Multi-Imager II (Alpha Innotech).
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