Uterus atrophy was checked to ensure the efficacy of the surgically-induced estrogen deficiency, by weighing uteri at the end of the experiment. After removing all soft tissue residue, left femurs were placed in a PBS buffer with 10% formaldehyde at 4 °C. Bone mineral density was measured using an eXplore CT 120 scanner (GE Healthcare, Canada). Acquisitions were performed with X-ray tube settings at 100 kV and 50 mA. We limited our investigation to the distal trabecular region. Bone mineral density (BMD) is presented in mgHA/cm3. Blood samples were collected in plain tubes and centrifuged at 10000G for 5 min at room temperature. The serum was subsequently isolated, aliquoted and stored at −80 °C. OPG (osteoprotegerin) and RANKL were measured by Quantikine ELISA for mouse (R&D Systems Europe).
Quantikine elisa for mouse
Manufactured by R&D Systems
The Quantikine ELISA for mouse is a quantitative sandwich enzyme immunoassay designed to measure mouse analytes in cell culture supernates, serum, and plasma. It provides a precise and reproducible method for the determination of specific mouse protein concentrations.
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2 protocols using quantikine elisa for mouse
1
Evaluating Estrogen Deficiency Impacts on Bone
2
Serum Biomarker Quantification in Mice
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Blood samples from 14-week-old mice were collected in tubes for serum chemistry, and centrifuged. Serum was isolated, aliquoted, and stored at -80ЊC. Osteoprotegerin (OPG), receptor activator of nuclear factor kappa-B ligand (RANKL), and monocyte chemoattractant protein 1 (MCP-1) were measured by Quantikine ELISA for mouse (R&D Systems Europe).
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