Polarstar omega plate

Manufactured by BMG Labtech

Sourced in Germany

The POLARstar Omega is a multimode microplate reader from BMG LABTECH. It is designed to perform various detection methods, including absorbance, fluorescence intensity, luminescence, and time-resolved fluorescence. The instrument provides accurate and reliable data for a wide range of applications in life science research and drug discovery.

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Lab products found in correlation

Prism 7, by GraphPad (11 mentions) Prism 8, by GraphPad (3 mentions) Celltiter glo luminescent cell viability assay, by Promega (1 mentions)

Spelling variants of this product

POLARstar Omega (453 citations) POLARstar Omega plate reader (138 citations) POLARstar Omega Plate Reader Spectrophotometer (7 citations) POLARstar OMEGA fluorescence plate reader (4 citations) POLARstar Omega microtitre plate reader (2 citations)

16 protocols using polarstar omega plate

Antifungal Susceptibility Assay for Candida albicans

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The fungus Candida albicans ATCC 10,231 was streaked onto a tryptic soy agar plate and incubated at 37 °C for 48 h. One colony was then transferred to fresh tryptic soy broth (5 mL) and the cell density was adjusted to 10⁴–10⁵ CFU/mL. Analytes (compounds) to be tested were dissolved in DMSO and diluted with H₂O to give a stock solution (600 μM in 20% DMSO), which was serially diluted with 20% DMSO to give concentrations from 600 μM to 0.2 μM in 20% DMSO. An aliquot (10 mL) of each dilution was transferred to a 96-well microtiter plate and freshly prepared fungal broth (190 μL) was added to each well to give final analyte concentrations ranging from 30 to 0.01 μM in 1% DMSO. The plates were incubated at 37 °C for 24 h and the optical density of each well measured spectrophotometrically at 600 nm using a POLARstar Omega plate (BMG LABTECH, Offenburg, Germany). Ketoconazole B was used as a positive control (30 μM in 1% DMSO). Where relevant, IC₅₀ value were calculated as the concentration of the compound or antifungal drug required for 50% inhibition of the fungal cells using Prism 8.0 (GraphPad Software Inc., La Jolla, CA, USA) (Figure S49).

Agampodi Dewa A., Khalil Z.G., Elbanna A.H, & Capon R.J. (2022). Chrysosporazines Revisited: Regioisomeric Phenylpropanoid Piperazine P-Glycoprotein Inhibitors from Australian Marine Fish-Derived Fungi. Molecules, 27(10), 3172.

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Antifungal Activity Screening of Compounds

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The fungus Candida albicans ATCC 10231 was streaked onto a Sabouraud agar plate and was incubated at 37 °C for 48 h. One colony was then transferred to fresh Sabouraud broth (15 mL) and the cell density adjusted to 10⁴–10⁵ CFU/mL. Test compounds were dissolved in DMSO and diluted with H₂O to give a 600 µM stock solution (20% DMSO), which was serially diluted with 20% DMSO to give concentrations from 600 to 0.2 µM in 20% DMSO. An aliquot (10 µL) of each dilution was transferred to a 96-well microtiter plate and freshly prepared fungal broth (190 µL) was added to each well to give final concentrations of 30 to 0.01 µM in 1% DMSO. The plates were incubated at 37 °C for 24 h and the optical density of each well was measured spectrophotometrically at 600 nm using POLARstar Omega plate (BMG LABTECH, Offenburg, Germany). Ketoconazole was used as a positive control (30 µg/mL in 10% DMSO). Where relevant, IC₅₀ value were calculated as the concentration of the compound or antifungal drug required for 50% inhibition of the fungal cells using Prism 7.0 (GraphPad Software Inc., La Jolla, CA, USA).

Elbanna A.H., Khalil Z.G., Bernhardt P.V, & Capon R.J. (2019). Scopularides Revisited: Molecular Networking Guided Exploration of Lipodepsipeptides in Australian Marine Fish Gastrointestinal Tract-Derived Fungi. Marine Drugs, 17(8), 475.

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Antibacterial Activity Screening of N-amino Anthranilic Acid

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The bacterium to be tested was streaked onto a tryptic soy agar plate and was incubated at 37 °C for 24 h. One colony was then transferred to fresh tryptic soy broth (5 mL), and the cell density was adjusted to 10⁴–10⁵ CFU/mL. N-amino anthranilic acid was dissolved in DMSO and diluted with H₂O to give 600 μM stock solution (20% DMSO), which was serially diluted with 20% DMSO to give concentrations from 600 μM to 0.2 μM in 20% DMSO. An aliquot (10 μL) of each dilution was transferred to a 96-well microtiter plate, and freshly prepared microbial broth (190 μL) was added to each well to give final concentrations of 30–0.01 μM in 1% DMSO. The plates were incubated at 37 °C for 24 h, and the optical density (OD) of each well was measured spectrophotometrically at 600 nm using POLARstar Omega plate (BMG LABTECH, Offenburg, Germany). Each test compound was screened against the Gram-negative bacterium Escherichia coli ATCC11775 and the Gram-positive bacteria Staphylococcus aureus ATCC25923 and Bacillus subtilis ATCC6633. A mixture of rifampicin and ampicillin was used as a positive control (30 μM in 20% DMSO). The IC₅₀ value was calculated as the concentration of the compound or antibiotic required for 50% inhibition of the bacterial cells using Prism 8.0 (GraphPad Software Inc., La Jolla, CA, USA) (Figure S26).

Khalil Z.G., Kankanamge S, & Capon R.J. (2022). Structure Revision of Penipacids A–E Reveals a Putative New Cryptic Natural Product, N-aminoanthranilic Acid, with Potential as a Transcriptional Regulator of Silent Secondary Metabolism. Marine Drugs, 20(6), 339.

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Antifungal Activity Screening of Compounds against Candida albicans

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The fungus Candida albicans ATCC 10231 was streaked onto a LB (Luria–Bertani) agar plate and was incubated at 37 °C for 48 h, after which a colony was transferred to fresh LB broth (15 mL) and the cell density was adjusted to 10⁴–10⁵ CFU/mL. Test compounds were dissolved in DMSO and diluted with H₂O to prepare 600 µM stock solutions (20% DMSO), which were serially diluted with 20% DMSO to provide concentrations from 600 µM to 0.2 µM in 20% DMSO. An aliquot (10 µL) of each dilution was transferred to a 96-well microtiter plate and freshly prepared fungal broth (190 µL) was added to prepare final concentrations of 30–0.01 µM in 1% DMSO. The plates were incubated at 27 °C for 48 h and the optical density of each well was measured spectrophotometrically at 600 nm using POLARstar Omega plate (BMG LABTECH, Offenburg, Germany). Amphotericin B was used as the positive control (40 µg/mL in 10% DMSO). The IC₅₀ value was calculated as the concentration of the compound or antibiotic required for 50% inhibition of the bacterial cells using Prism 7.0 (GraphPad Software Inc., La Jolla, CA, USA). See Figure S80.

Kankanamge S., Khalil Z.G., Bernhardt P.V, & Capon R.J. (2022). Noonindoles A–F: Rare Indole Diterpene Amino Acid Conjugates from a Marine-Derived Fungus, Aspergillus noonimiae CMB-M0339. Marine Drugs, 20(11), 698.

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Antifungal Activity Screening of Test Compounds against Candida albicans

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The fungus Candida albicans ATCC 10231 was streaked onto a Sabouraud agar plate and was incubated at 37 °C for 48 h. One colony was then transferred to fresh Sabouraud broth (15 mL) and the cell density adjusted to 10⁴–10⁵ CFU/mL. Test compounds were dissolved in DMSO and diluted with H₂O to give a 600 µM stock solution (20% DMSO), which was serially diluted with 20% DMSO to give concentrations from 600 µM to 0.2 µM in 20% DMSO. An aliquot (10 µL) of each dilution was transferred to a 96-well microtiter plate and freshly prepared fungal broth (190 µL) was added to each well to give final concentrations of 30–0.01 µM in 1% DMSO. The plates were incubated at 37 °C for 24 h and the optical density of each well was measured spectrophotometrically at 600 nm using a POLARstar Omega plate (BMG LABTECH, Offenburg, Germany). Ketoconazole was used as a positive control (30 µg/ml in 10% DMSO). Where relevant, IC₅₀ values were calculated as the concentration of the compound or antifungal drug required for 50% inhibition of the fungal cells using Prism 7.0 (GraphPad Software Inc., La Jolla, CA, USA) (Figure S67).

Elbanna A.H., Agampodi Dewa A., Khalil Z.G, & Capon R.J. (2021). Precursor-Directed Biosynthesis Mediated Amplification of Minor Aza Phenylpropanoid Piperazines in an Australian Marine Fish-Gut-Derived Fungus, Chrysosporium sp. CMB-F214. Marine Drugs, 19(9), 478.

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Antimicrobial Susceptibility Testing Protocol

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The bacterium to be tested was streaked onto a tryptic soy agar plate and incubated at 28 °C or 37 °C for 24 h. One colony was then transferred to fresh tryptic soy broth (Difco, USA) (15 mL) and the cell density was adjusted to 10⁴–10⁵ colony-forming units (cfu)/mL. Test extracts were dissolved in 100% dimethyl sulfoxide (DMSO) and diluted with sterilised deionised water to 10% DMSO. The stock solution was diluted with 10% DMSO to give final concentrations of 1% DMSO. The extract concentrations were then serially diluted to 500, 250, 150, and 100 µg extracts/mL of 1% DMSO. Each dilution was transferred to a 96-well microtiter plate and freshly prepared microbial broth was added to each well. Negative growth controls contained only growth media, and positive growth controls contained growth media and bacterial suspension. The plates were incubated at 37 °C for 24 h, and the optical density of each well was measured spectrophotometrically at 600 nm (using POLARstar Omega Plate, BMG LABTECH, Offenburg, Germany). MIC values were determined after overnight incubation and recorded as the lowest concentration of each test solution that inhibited the growth of all the microorganisms in the wells. The experiments were conducted in triplicate for each sample and the average concentration of each triplicate was calculated.

Alsenani F., Tupally K.R., Chua E.T., Eltanahy E., Alsufyani H., Parekh H.S, & Schenk P.M. (2020). Evaluation of microalgae and cyanobacteria as potential sources of antimicrobial compounds. Saudi Pharmaceutical Journal : SPJ, 28(12), 1834-1841.

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Cell Viability Assay with CellTiterGlo

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Cells were seeded in 96-well plates with indicated concentrations of respective compounds and plates were incubated at 37 °C for 96 h. Readout was performed with CellTiterGlo® Luminescent Cell Viability Assay (Promega) according to the manufacturer’s protocol and luminescence was measured with POLARStar Omega plate reader (BMG LabTech). All results were normalized to non-treated conditions and data represent mean ± SD of biological triplicates.

Wang Z., Shaabani S., Gao X., Ng Y.L., Sapozhnikova V., Mertins P., Krönke J, & Dömling A. (2023). Direct-to-biology, automated, nano-scale synthesis, and phenotypic screening-enabled E3 ligase modulator discovery. Nature Communications, 14, 8437.

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Antifungal Activity Evaluation of N-amino Anthranilic Acid

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The fungus Candida albicans ATCC 10231 was streaked onto a tryptic soy agar plate and was incubated at 37 °C for 48 h. One colony was then transferred to fresh tryptic soy broth (5 mL), and the cell density was adjusted to 10⁴–10⁵ CFU/mL. N-amino anthranilic acid was dissolved in DMSO and diluted with H₂O to give a 600 μM stock solution (20% DMSO), which was serially diluted with 20% DMSO to give concentrations from 600 μM to 0.2 μM in 20% DMSO. An aliquot (10 μL) of each dilution was transferred to a 96-well microtiter plate, and freshly prepared fungal broth (190 μL) was added to each well to give final concentrations of 30–0.01 μM in 1% DMSO. The plates were incubated at 37 °C for 24 h, and the optical density (OD) of each well was measured spectrophotometrically at 600 nm using POLARstar Omega plate (BMG LABTECH, Offenburg, Germany). Amphotericin B was used as a positive control (30 μg/mL in 20% DMSO). Where relevant, IC₅₀ value were calculated as the concentration of the compound or antifungal drug required for 50% inhibition of the fungal cells using Prism 8.0 (GraphPad Software Inc., La Jolla, CA, USA) (Figure S26).

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Antifungal activity screening of C. albicans

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The fungus C. albicans ATCC10231 was streaked onto a Sabouraud agar plate and was incubated
at 37 °C for 48 h. One colony was then transferred to fresh Sabouraud
broth (15 mL) and the cell density adjusted to 10⁴–10⁵ CFU/mL. For the single data point experiments, an aliquot
(10 μL) from 600 μM stock solution (20% DMSO) was transferred
to a 96-well microtiter plate, and freshly prepared fungal broth (190
μL) was added to each well to give final concentrations of 30
μM in 1% DMSO. The plates were incubated at 37 °C for 24
h, and the optical density of each well was measured spectrophotometrically
at 600 nm using a POLARstar Omega plate (BMG LABTECH, Offenburg, Germany).
Ketoconazole was used as a positive control (30 μg/mL in 10%
DMSO). Where relevant, IC₅₀ values were calculated as the
concentration of the compound or antifungal drug required for 50%
inhibition of the fungal cells using Prism 7.0 (GraphPad Software
Inc., La Jolla, CA).

Liu X., Carr P., Gardiner M.G., Banwell M.G., Elbanna A.H., Khalil Z.G, & Capon R.J. (2020). Levoglucosenone and Its Pseudoenantiomer iso-Levoglucosenone as Scaffolds for Drug Discovery and Development. ACS Omega, 5(23), 13926-13939.

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Antimicrobial Susceptibility Screening

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The bacterium to be tested was streaked onto a tryptic soy agar plate and incubated at 37 °C for 24 h. One colony was then transferred to fresh tryptic soy broth (15 mL) and the cell density adjusted to 10⁴–10⁵ CFU/mL. The compounds to be tested were dissolved in DMSO and diluted with H₂O to return 600 µM stock solutions (20% DMSO). The stock solutions were serially diluted to give concentration range of 600 µM to 0.2 µM in 20% DMSO/H₂O. An aliquot (10 µL) of each dilution was transferred to a 96-well microtiter plate and freshly prepared microbial broth (190 µL) was added to each well. The plates were incubated at 37 °C for 24 h and the optical density of each well was measured spectrophotometrically at 600 nm using POLARstar Omega plate (BMG LABTECH, Offenburg, Germany). Each test compound was screened against the Gram-negative bacterium Escherichia coli (ATCC 11775) and the Gram-positive bacterium Bacillus subtilis (ATCC 6051). Rifampicin was used as a positive control (40 µg/mL in 10% DMSO). The IC₅₀ value was calculated as the concentration of the compound or antibiotic required for 50% inhibition of the bacterial cells using Prism 7.0 from GraphPad Software Inc. (La Jolla, CA, USA).

Khushi S., Nahar L., Salim A.A, & Capon R.J. (2018). Cacolides: Sesterterpene Butenolides from a Southern Australian Marine Sponge, Cacospongia sp.. Marine Drugs, 16(11), 456.

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