Aggregate samples containing 1E6 cells were removed from the bioreactor culture and added into microcentrifuge tubes (Cat#10011-724, VWR). Aggregates were rinsed twice with PBS and resuspended in 0.5 mL of fixation buffer (Cat#FC001, R&D Systems) to be incubated for 1 h at room temperature. Aggregate samples were then rinsed twice with PBS and resuspended in 200-μL permeabilization buffer (Cat#FC005, R&D Systems) with 1 μg/106 cells antibody stain and 1 μM/106 cells nuclei stain and incubated for 3 h at room temperature. Conjugated antibody stains for SSEA-4 (Cat#FAB1435F, R&D Systems), TRA-1-60 (Cat# FAB4770P, R&D Systems), and Nanog (Cat#MABD24A4, Millipore Sigma) were used along with the nuclei stain To-Pro-3 Iodide Nucleic Acid Stain (Cat#T3605, Thermo Fisher). Cells were then rinsed twice with PBS and imaged using a Carl Zeiss Laser Scanning Microscope 700 with lasers at 488 nm and 639 nm and corresponding filter sets.
Mabd24a4
Manufactured by Merck Group
The MABD24A4 is a compact and versatile laboratory device designed for various analytical applications. It features precise temperature control and high-speed data acquisition capabilities. The core function of this product is to facilitate accurate and reliable measurements in a laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
Microcentrifuge tube, by Avantor (3 mentions)
Laser scanning microscope 700, by Zeiss (3 mentions)
Fab1435f, by R&D Systems (3 mentions)
Fc001, by R&D Systems (3 mentions)
Fab4770p, by R&D Systems (3 mentions)
To pro 3 iodide nucleic acid stain, by Thermo Fisher Scientific (3 mentions)
Fc005, by R&D Systems (3 mentions)
3 protocols using mabd24a4
1
Immunofluorescence Analysis of Stem Cells
2
Immunophenotyping of Stem Cell Aggregates
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Aggregate samples containing 1E6 cells were removed from the bioreactor culture and added into microcentrifuge tubes (Cat#10011-724, VWR). Aggregates were rinsed twice with PBS and resuspended in 0.5 mL of fixation buffer (Cat#FC001, R&D Systems) to be incubated for 1 hr at room temperature. Aggregate samples where then rinsed twice with PBS and resuspended in 200 L permeabilization buffer (Cat#FC005, R&D Systems) with 1 g/10 6 cells antibody stain and 1 M/10 6 cells nuclei stain and incubated for 3 hrs at room temperature.
Conjugated antibody stains for SSEA-4 (Cat#FAB1435F, R&D Systems), TRA-1-60 (Cat# FAB4770P, R&D Systems) and Nanog (Cat#MABD24A4, Millipore Sigma) were used along with the nuclei stain To-Pro-3 Iodide Nucleic Acid Stain (Cat#T3605, Thermo Fisher). Cells were then rinsed twice with PBS and imaged using a Carl Zeiss Laser Scanning Microscope 700 with lasers at 488 nm and 639 nm and corresponding filter sets.
Conjugated antibody stains for SSEA-4 (Cat#FAB1435F, R&D Systems), TRA-1-60 (Cat# FAB4770P, R&D Systems) and Nanog (Cat#MABD24A4, Millipore Sigma) were used along with the nuclei stain To-Pro-3 Iodide Nucleic Acid Stain (Cat#T3605, Thermo Fisher). Cells were then rinsed twice with PBS and imaged using a Carl Zeiss Laser Scanning Microscope 700 with lasers at 488 nm and 639 nm and corresponding filter sets.
3
Pluripotent Stem Cell Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Aggregate samples containing 1E6 cells were removed from the bioreactor culture and added into microcentrifuge tubes (Cat#10011-724, VWR). Aggregates were rinsed twice with PBS and resuspended in 0.5 mL of fixation buffer (Cat#FC001, R&D Systems) to be incubated for 1 hr at room temperature. Aggregate samples where then rinsed twice with PBS and resuspended in 200 L permeabilization buffer (Cat#FC005, R&D Systems) with 1 g/10 6 cells antibody stain and 1 M/10 6 cells nuclei stain and incubated for 3 hrs at room temperature. Conjugated antibody stains for SSEA-4 (Cat#FAB1435F, R&D Systems), TRA-1-60 (Cat# FAB4770P, R&D Systems)
and Nanog (Cat#MABD24A4, Millipore Sigma) were used along with the nuclei stain To-Pro-3
Iodide Nucleic Acid Stain (Cat#T3605, Thermo Fisher). Cells were then rinsed twice with PBS and imaged using a Carl Zeiss Laser Scanning Microscope 700 with lasers at 488 nm and 639 nm and corresponding filter sets.
and Nanog (Cat#MABD24A4, Millipore Sigma) were used along with the nuclei stain To-Pro-3
Iodide Nucleic Acid Stain (Cat#T3605, Thermo Fisher). Cells were then rinsed twice with PBS and imaged using a Carl Zeiss Laser Scanning Microscope 700 with lasers at 488 nm and 639 nm and corresponding filter sets.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!