Human bladder cancer cells (UM-UC-3, J82, and BIU-87) were purchased from ATCC. All cells were cultured in RPMI 1640 medium (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, maintained in humidified air containing 5% CO2 at 37°C. The tumor tissue samples of BC patients were obtained from First Affiliated Hospital of Zhejiang University. All patients received written informed consent and the study protocol was approved by the Clinical Research Ethics Committee of the First Affiliated Hospital of Zhejiang University.
Biu 87
Manufactured by American Type Culture Collection
Sourced in United States
The BIU-87 is a laboratory equipment designed for the cultivation of microorganisms. It provides a controlled environment with regulated temperature, humidity, and air circulation to support the growth of various microbial cultures. The BIU-87 is a standardized piece of equipment used in microbiology research and testing facilities.
Automatically generated - may contain errors
Lab products found in correlation
Sv huc 1, by American Type Culture Collection (20 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (16 mentions)
Umuc3, by American Type Culture Collection (15 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (14 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (10 mentions)
Streptomycin, by Thermo Fisher Scientific (7 mentions)
Penicillin, by Thermo Fisher Scientific (7 mentions)
Rpmi 1640, by Thermo Fisher Scientific (6 mentions)
Sw780, by American Type Culture Collection (4 mentions)
Rt112, by American Type Culture Collection (4 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (4 mentions)
Tccsup, by American Type Culture Collection (3 mentions)
Scaber, by American Type Culture Collection (3 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (3 mentions)
Hcv29, by American Type Culture Collection (3 mentions)
Dulbecco modified eagle medium (dmem), by Corning (2 mentions)
Attractene transfection reagent, by Qiagen (2 mentions)
Biu 87, by Thermo Fisher Scientific (2 mentions)
Sv huc 1, by Thermo Fisher Scientific (2 mentions)
F 12k medium, by Thermo Fisher Scientific (2 mentions)
46 protocols using biu 87
1
Culturing Bladder Cancer Cells
2
Culturing Bladder Cancer Cell Lines
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The human BC cells (BIU87, 5637, T24, EJ, RT4, J82, UM-UC-3, TCCSUP), and SVHUC-1, a normal bladder uroepithelial cell line, were purchased from the ATCC (Rockville, MD, USA). 5637 and SV-HUC-1 cells were cultured in RPMI-1640 and F12K medium, respectively. Other cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM), both including 10% FBS. Dulbecco’s Phosphate-Buffered Saline (DPBS) without Ca2+ and Mg2+ was used to wash the cells.
3
Bladder Cancer Cell Line Cultivation
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Human bladder cancer cell lines BIU-87, J82, and UM-UC-3 were purchased from the ATCC (Manassas, VA, USA). The cell lines were cultured in DMEM (Gibco, Carlsbad, CA, USA) supplemented with 10% FBS and 1% penicillin/streptomycin. All cells were maintained at 37°C in 5% CO2/95% air. Doxorubicin and GC7 were purchased from Sigma-Aldrich (St. Louis, MO, USA). Polyamine spermidine and spermine were purchased from Sigma-Aldrich. The eIF5A2 and Twist-1 siRNA and negative control siRNA were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
4
Cell Culture Protocols for Bladder Cancer
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The cell lines involved in this experiment, including T24, 5637, EJ, BIU87, and SV-HUC-1, were purchased from ATCC. These cells were cultured in RPMI 1640 medium (Gibco, Waltham, MA, USA) containing 10% foetal bovine serum (Gibco, Waltham, MA, USA) with culture conditions of 37.0°C with 5% CO2.
5
Culturing Human Bladder Cell Lines
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Normal human SVHUC-1 bladder epithelial cells (HUCL-022, iCell Bioscience Inc., Shanghai, China) and human BC cells, EJ, T24, BIU87, ScaBER and 5637 (ATCC 1089, ATCC 1057, QDC110, ZY-H792, ATCC-9532, respectively), from the American Type Culture Collection (ATCC, Manassas, VA, USA) were cultured in RPMI 1640 medium (22400089, Gibco Company, Grand Island, NY, USA) containing 10% fetal bovine serum. The cells were seeded into a six-well plate at a density of 1 × 105 cells per well and incubated at 37 °C with 5% CO2 and saturated humidity. The medium was changed every 2–3 days. The cells were passaged if the confluence reached 80–90%. After the medium was discarded, the cells were washed twice with phosphate-buffered saline (PBS) and digested with 0.25% trypsin for 2~5 min. The cells were suspended in 5 ml of Dulbecco’s Modified Eagle’s medium (190040, Gibco Company) containing 10% fetal bovine serum and then subcultured.
6
Culturing and Transfecting Bladder Cancer Cell Lines
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UBC cancer cell lines UM-UC-3, SW780, T24, J82, BIU-87, 5637 (ATCC, Manassas, VA, USA) were acquired from the Fujian Medical University Union Hospital. All types of tumor cell lines were cultured utilizing RPMI-1640 medium (Gibco, Langley, OK, USA) combined with 10% fetal bovine serum (Gibco, Langley, OK, USA). All cells were cultured at 37°C with 5% CO2 incubator environment. Vector transfection was conducted using Lipofectamine 3000 Reagent (Invitrogen, Waltham, MA, USA), and transient transfection was constructed.
7
Cell Culture and Transfection Protocols
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The human BCa cell lines J82, BC-5367, UMUC3, BIU-87, and T24 and human non-cancerous immortalized urothelial cell line SV-HUC-1 were purchased from ATCC and cultured in Dulbecco’s modified Eagle’s medium (DMEM; HyClone, United States) containing 10% fetal bovine serum (FBS; HyClone, United States) and 1% penicillin/streptomycin. The cells were incubated in a humid atmosphere with 5% CO2 at 37°C.
Cell transfection was performed using the Lipofectamine 2000TM reagent (Invitrogen, United States) according to the manufacturer’s instructions.
Cell transfection was performed using the Lipofectamine 2000TM reagent (Invitrogen, United States) according to the manufacturer’s instructions.
8
Inhibition of Bladder Cancer Cells
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Human bladder cancer cells Biu87 and T24 were purchased from ATCC. All cells were maintained in RPMI‐1640 complete medium (Gibco) supplemented with 10% FCS (Gibco) at 37°C in a humidified, 5% CO2 atmosphere. Mitomycin C (MMC) and hydroxycamptothecin (HCPT) were purchased from Sangon. C‐Myc inhibitor 10058‐F4, NF‐κB inhibitor E330 and integrin inhibitor Arg‐Gly‐Asp‐Ser (AGAS) were purchased from MedChemExpress. Other reagents were of HPLC standard and purchased from Solarbio.
9
Molecular Profiling of Bladder Cancer Cell Lines
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SV-HUC-1, BIU-87, 5637, and T24 cell lines were purchased from American Type Culture Collection (ATCC; Manassas, VA), which were cultured in 1640 medium (Invitrogen, Carlsbad, CA) with 10% fetal bovine serum (Invitrogen). Cells were passaged every 2 days with trypsin.
DharmaFECT1 (Dharmacon, Lafayette, CO) was used for siRNA knockdown. ONTARGETplus siRNA for TRIM29 was also obtained from Dharmacon. Non-targeting siRNAa were used as negative control.
pCMV6-TRIM29 plasmid was obtained from Origene (Rockville, MD). Attractene Transfection was used for transfection of plasmid (Qiagen, Hilden, Germany). pCMV6 was used as control.
BAY 11-7082 (Sigma, St. Louis, MO) was used as NF-κB inhibitor. Protein kinase C (PKC) inhibitor staurosporine was purchased from Beyotime Biotechnology (Shanghai, China).
DharmaFECT1 (Dharmacon, Lafayette, CO) was used for siRNA knockdown. ONTARGETplus siRNA for TRIM29 was also obtained from Dharmacon. Non-targeting siRNAa were used as negative control.
pCMV6-TRIM29 plasmid was obtained from Origene (Rockville, MD). Attractene Transfection was used for transfection of plasmid (Qiagen, Hilden, Germany). pCMV6 was used as control.
BAY 11-7082 (Sigma, St. Louis, MO) was used as NF-κB inhibitor. Protein kinase C (PKC) inhibitor staurosporine was purchased from Beyotime Biotechnology (Shanghai, China).
10
Bladder Cancer Cell Response to TGF-β1
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Human bladder cancer cell lines (BIU-87, UMUC3, and 5637) and normal bladder epithelial cell (SV-HUC-1) were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA). They were cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Gibco), 100 U/ml penicillin, and 100 U/ml streptomycin under a humidified incubator that was maintained at 37°C and supplied with 5% CO2 and 95% air. The bladder cancer cells were then treated with TGF-β1 (10 ng/ml) for different times.
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