Human H1299 cells were obtained from the American Type Culture Collection (ATCC). Cell culture (RPMI) media, fetal bovine serum (FBS), trypsin, antibiotic, and Hoechst 33342 were from Thermo Fisher Scientific (Burlington, ON, Canada). Antibodies against caspase-3 (cat. No. 9662), caspase-7 (cat. No. 9494), PARP (cat. No. 9542), BAX (cat. No. 2772), Bcl-2 (cat. No. 2872), β-actin (cat. No. 4967), sestrin-2 (cat. No. 8487), pLKB1, LKB1 (cat. No. 3047), pAMPK (cat. No. 2535), AMPK (cat. No. 5831), and LC3 (cat. No. 12741) were purchased from Cell Signaling Technology via New England Biolabs (Mississauga, ON, Canada). Bovine serum albumin (BSA), 10% formalin, dimethyl sulfoxide (DMSO), crystal violet stain, and paclitaxel were purchased from Millipore Sigma (Oakville, ON, Canada). Carnosic acid (purity 98.17%) and 3-methyladenine were purchased from Med Chem Express (Monmouth Junction, NJ, USA).
Human h1299 cells
Manufactured by American Type Culture Collection
Sourced in United States
The Human H1299 cells are a type of non-small cell lung cancer cell line derived from the lymph node metastasis of a lung carcinoma. These cells are commonly used in research applications to study lung cancer biology and for the evaluation of potential therapeutic interventions.
Automatically generated - may contain errors
Lab products found in correlation
Hoechst 33342, by Thermo Fisher Scientific (2 mentions)
3 methyladenine, by MedChemExpress (1 mentions)
Carnosic acid, by MedChemExpress (1 mentions)
Paclitaxel, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Mitoprobe jc 1 assay kit, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Q5 site directed mutagenesis kit, by New England Biolabs (1 mentions)
2 protocols using human h1299 cells
1
Carnosic Acid Induces Apoptosis in H1299 Cells
2
Cell Culture and Transfection Protocols
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Human H1299 cells were obtained from the American Type Culture Collection and
maintained in RPMI-1640 medium (Gibco, Waltham, MA, USA) containing 10% fetal
bovine serum (Gibco, Waltham, MA, USA) and 1% penicillin/streptomycin
(Invitrogen, Carlsbad, CA, USA). SAHA was obtained from Sigma-Aldrich (St.
Louis, MO, USA). Lipofectamine 2000 and the MitoProbe™ JC-1 Assay Kit were
purchased from Invitrogen (Carlsbad, CA, USA). The Q5® Site-Directed Mutagenesis
Kit was purchased from New England Biolabs (Ipswich, MA, USA). Ethical approval
was not applied for because this study did not involve patients or animals.
maintained in RPMI-1640 medium (Gibco, Waltham, MA, USA) containing 10% fetal
bovine serum (Gibco, Waltham, MA, USA) and 1% penicillin/streptomycin
(Invitrogen, Carlsbad, CA, USA). SAHA was obtained from Sigma-Aldrich (St.
Louis, MO, USA). Lipofectamine 2000 and the MitoProbe™ JC-1 Assay Kit were
purchased from Invitrogen (Carlsbad, CA, USA). The Q5® Site-Directed Mutagenesis
Kit was purchased from New England Biolabs (Ipswich, MA, USA). Ethical approval
was not applied for because this study did not involve patients or animals.
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