Log In Sign up
The largest database of trusted experimental protocols

Nih 3t3 fibroblasts cell line

Manufactured by American Type Culture Collection
Visit Supplier
Sourced in United States

The NIH 3T3 fibroblasts cell line is a standardized mouse embryonic fibroblast cell line. It is a widely used in vitro model for research purposes.

Automatically generated - may contain errors

Lab products found in correlation

Ethylenediaminetetraacetic acid, by Solarbio (1 mentions) Dmem high glucose, by Merck Group (1 mentions) Penicillin streptomycin, by Solarbio (1 mentions) Trypsin, by Solarbio (1 mentions) Atcc crl 1658, by American Type Culture Collection (1 mentions)

2 protocols using nih 3t3 fibroblasts cell line

1

In Vitro Cytotoxicity Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sigma (USA) provided fetal bovine serum (FBS), dimethylsulfoxide (DMSO), Dulbecco’s Modified Eagle’s Medium (DMEM) High Glucose, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT). The American Type Culture Collection (ATCC) provided NIH 3T3 fibroblasts cell line. Penicillin/streptomycin and 0.25% (w/v) trypsin-0.1% (w/v) ethylenediaminetetraacetic acid (EDTA) was purchased from Solarbio (Beijing Solarbio Science and Technology, China).
Rahmani E., Pourmadadi M., Zandi N., Rahdar A, & Baino F. (2022). pH-Responsive PVA-Based Nanofibers Containing GO Modified with Ag Nanoparticles: Physico-Chemical Characterization, Wound Dressing, and Drug Delivery. Micromachines, 13(11), 1847.
+ Open protocol
+ Expand
2

Biocompatibility Assessment of Plant Extracts

Check if the same lab product or an alternative is used in the 5 most similar protocols
The biocompatibility of both plant extracts was assessed on NIH/3T3 fibroblasts cell line (ATCC CRL-1658; American Type Culture Collection, Rockville, MD, USA) using the Sulforhodamine B (SRB) assay. The NIH/3T3 cells were exposed for 48 h to different concentrations (15.5, 31, 62.5, and 125 µg/mL) of tested extracts added to the cell culture medium, or to the vehicle alone (DMSO) used as the control [45 (link)].
Bouabidi M., Salamone F.L., Gadhi C., Bouamama H., Speciale A., Ginestra G., Pulvirenti L., Siracusa L., Nostro A, & Cristani M. (2023). Efficacy of Two Moroccan Cistus Species Extracts against Acne Vulgaris: Phytochemical Profile, Antioxidant, Anti-Inflammatory and Antimicrobial Activities. Molecules, 28(6), 2797.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!