Total cellular RNA was extracted from MCF-7 cells, L02 cells or HepG2 cells using Trizol reagent S5 (Sangon Co. Ltd., Shanghai, China) according to the indicated protocol. The cDNA samples were prepared by using the reverse transcription (RT) reaction with an AMV First Strand cDNA Synthesis Kit (BBI, Toronto, Canada). qPCR analysis of mRNA was performed with SG Fast qPCR Master Mix (2X) (BBI), according to the indicated protocol on an LightCycler 480 Software Setup (Roche). The primers used in this experiment were shown in Table S1.† We evaluated all of the data with respect to the mRNA expression by normalizing to the expression of GAPDH and using the 2–ΔΔCt method.
Trizol reagent s5
Manufactured by Sangon
Sourced in China
Trizol reagent S5 is a ready-to-use solution for the isolation of high-quality total RNA from a variety of biological samples. It is a monophasic solution of phenol and guanidine isothiocyanate that effectively lyses cells and disrupts nucleoprotein complexes, allowing the extraction and purification of RNA.
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Lab products found in correlation
2 protocols using trizol reagent s5
1
Quantifying mRNA Expression in Cell Lines
2
Quantitative RT-PCR Analysis of mRNA
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Total cellular RNA was extracted from HepG2 cells or L02 cells using Trizol reagent S5 (Sangon Co. Ltd., Shanghai, China) according to the indicated protocol. The cDNA samples were prepared by using the reverse transcription (RT) reaction with AMV First Strand cDNA Synthesis Kit (BBI, Toronto, Canada). qRT-PCR analysis of mRNA was performed with SG Fast qPCR Master Mix (2X) (BBI) on a LightCycler480 Software Setup (Roche). The primers used in this experiment were shown in Table S1.† We evaluated all the data with respect to the mRNA expression by normalizing to the expression of GAPDH and using the 2–ΔΔCt method.
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