Andor Revolution XD spinning disk confocal microscopy was used for the image acquisition. The microscope was equipped with a Nikon Eclipse Ti inverted microscope, Nikon Plan Apo Lambda 100×/1.45-NA oil-immersion objective lens, a spinning-disk system (CSU-X1; Yokogawa Electric Corporation), and an Andor iXon Ultra EMCCD camera. Images were acquired using the Andor IQ3 software at 80 nm/pixel. The fluorophores were excited by laser lines at wavelengths of 488 or 561 nm. Most images were acquired with z-step sizes of 0.2, 0.3, or 0.5 µm, at various interval times indicated in individual time-lapse videos.
Plan apo lambda 100 1.45 na
Manufactured by Yokogawa
The Plan Apo Lambda 100×/1.45-NA is a high-performance microscope objective lens designed for advanced imaging applications. It features a numerical aperture of 1.45 and a magnification of 100x, providing exceptional optical performance and resolution. The lens is optimized for use with the Plan Apo Lambda series of objectives from Yokogawa.
Automatically generated - may contain errors
Lab products found in correlation
Ixon ultra emccd camera, by Oxford Instruments (2 mentions)
Eclipse ti, by Nikon (2 mentions)
Csu x1, by Yokogawa (2 mentions)
Zyla 4.2 scmos camera, by Oxford Instruments (1 mentions)
Nis elements v4, by Nikon (1 mentions)
Csu w1, by Yokogawa (1 mentions)
Revolution xd, by Oxford Instruments (1 mentions)
3 protocols using plan apo lambda 100 1.45 na
1
Spinning Disk Confocal Microscopy Protocol
2
Confocal Microscopy Imaging of Cells
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Immunofluorescence images and DAPI staining images were acquired every 0.2 μm intervals of z-slice using a Zyla 4.2 sCMOS camera (Andor) mounted on a Nikon Eclipse Ti inverted microscope with an objective lens (Nikon; Plan Apo lambda 100×/1.45 NA) with a spinning disk confocal scanner unit (CSU-W1; Yokogawa) controlled with NIS-elements v4.60 (Nikon). The images in the Figures are the maximum intensity projection or sum intensity projection (chromosome spread samples) of the Z-stack generated with Fiji (v1.53)70 (link) and were processed using Fiji (v1.53) and Adobe Photoshop v23.1.0 (Adobe).
3
Spinning Disk Confocal Microscopy for Fluorescence Imaging
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Image acquisition was performed using Andor Revolution XD spinning disk confocal microscopy. The microscope was equipped with a Nikon Eclipse Ti inverted microscope, Nikon Plan Apo Lambda 100×/1.45-NA oil-immersion objective lens, a spinning-disk system (CSU-X1; Yokogawa Electric Corporation), and an Andor iXon Ultra EMCCD camera. Andor IQ3 software was used to acquire images at 80 nm/pixel. Laser lines at wavelengths of 488 or 561 nm were used to excite the fluorophores. Most images were acquired with z-step sizes of 0.2, 0.3, or 0.5 µm, and at various interval times.
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