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Dmr optical microscope

Manufactured by Leica
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Sourced in Germany

The Leica DMR optical microscope is a versatile instrument designed for high-quality imaging and analysis. It features a modular design that allows for customization to meet various research and laboratory needs. The core function of the DMR is to provide users with a reliable and precise platform for magnifying and observing samples.

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Lab products found in correlation

Amira software, by Thermo Fisher Scientific (2 mentions) Histo jumbo knife, by Electron Microscopy Sciences (2 mentions) Stereo investigator, by MicroBrightField (1 mentions)

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Optical microscope (343 citations) DMR microscope (236 citations)

5 protocols using dmr optical microscope

1

Egg Development of Aplysia kurodai

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Adult A. kurodai were collected in the intertidal zone along the coastal waters of the Hamduk, northeast of Jeju Island, Korea. Adult animals were kept at the Marine Science Institute, Jeju National University, Jeju, Korea, in 5 ton aquaria with an open seawater circulation system, and fed daily fresh algae (Ulva sp.). Newly spawned egg clusters were collected from the aquaria, rinsed, and inserted into 1-L flasks containing filtered seawater (filter holes diameter 0.45 μm) under continuous aeration. The filtered seawater was changed daily and was maintained at room temperature (20±0.5°C) and salinity (31.9±0.6 PSU). The structure of egg mass and egg development t of A. kurodai were viewed with a Leica DMR optical microscope.
Lee C.H., Kaang B.K, & Lee Y.D. (2014). Spawning Behavior and Egg Development of Aplysia kurodai Inhabiting the Coastal Waters of Jeju Island, Korea. Development & Reproduction, 18(1), 25-31.
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2

Fungal Caox Crystal Production Evaluation

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Using a malt (12 g·L−1) agar (15 g·L−1) medium supplemented with 5 g·L−1 CaCO3, we evaluated the production of Caox crystals for each fungal strain with a Leica DMR optical microscope and a ×400 magnification, following the procedure described in [32 (link)]. Because fungal strains grew at different rates, we observed the fungal colonies daily and sampled them for screening only when they just reached the periphery of the plates. For each plate, we observed both the youngest (at the periphery of the plate) and the oldest part (at the center of the plate) of the mycelia.
Hervé V., Simon A., Randevoson F., Cailleau G., Rajoelison G., Razakamanarivo H., Bindschedler S., Verrecchia E, & Junier P. (2021). Functional Diversity of the Litter-Associated Fungi from an Oxalate-Carbonate Pathway Ecosystem in Madagascar. Microorganisms, 9(5), 985.
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3

3D Reconstruction of Tunicate Metamorphosis

Cited 1 time
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An oozooid (E9), a bud and secondary bud (B1+b1) and a larva in early metamorphosis (E7-E8), were embedded in resin as previously described and serially transversely cut using a Histo Jumbo Knife (Diatome). Sections, 1 µm thick, were arranged in chains of about 20 sections each and stained with toluidine blue. All the sections were then photographed with Leica DMR optical microscope. Images were aligned using Adobe Photoshop CS on a Windows 7 computer. Based on the resulting stack of images, 3D models of the anatomy of all organ systems were created in Amira software (Thermofisher scientific). Reconstructions were made in 3D of a larva in early metamorphosis (E7-E8), an oozooid (E9), and a primary bud and secondary bud (B1+b1) using 426, 853, and 375 sections respectively. Their organs and tissues can be visualized using the MorphoNet browser (Figures 1G–1I; Figure S1) (Leggio et al., 2019 (link)).
Kowarsky M., Anselmi C., Hotta K., Burighel P., Zaniolo G., Caicci F., Rosental B., Neff N.F., Ishizuka K.J., Palmeri K.J., Okamoto J., Gordon T., Weissman I.L., Quake S.R., Manni L, & Voskoboynik A. (2021). Sexual and asexual development: two distinct programs producing the same tunicate. Cell reports, 34(4), 108681.
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4

3D Reconstruction of Tunicate Metamorphosis

Cited 1 time
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An oozooid (E9), a bud and secondary bud (B1+b1) and a larva in early metamorphosis (E7-E8), were embedded in resin as previously described and serially transversely cut using a Histo Jumbo Knife (Diatome). Sections, 1 µm thick, were arranged in chains of about 20 sections each and stained with toluidine blue. All the sections were then photographed with Leica DMR optical microscope. Images were aligned using Adobe Photoshop CS on a Windows 7 computer. Based on the resulting stack of images, 3D models of the anatomy of all organ systems were created in Amira software (Thermofisher scientific). Reconstructions were made in 3D of a larva in early metamorphosis (E7-E8), an oozooid (E9), and a primary bud and secondary bud (B1+b1) using 426, 853, and 375 sections respectively. Their organs and tissues can be visualized using the MorphoNet browser (Figures 1G–1I; Figure S1) (Leggio et al., 2019 (link)).
Kowarsky M., Anselmi C., Hotta K., Burighel P., Zaniolo G., Caicci F., Rosental B., Neff N.F., Ishizuka K.J., Palmeri K.J., Okamoto J., Gordon T., Weissman I.L., Quake S.R., Manni L, & Voskoboynik A. (2021). Sexual and asexual development: two distinct programs producing the same tunicate. Cell reports, 34(4), 108681.
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5

Quantifying Dopaminergic Neurons in Parkinson's Model

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The total number of immunoreactive positive cells in the SN was estimated by stereological measurements using the optical fractionator method in a computerized system (StereoInvestigator; MicroBright-Field, Magdeburg, Germany) and a Leica DMR optical microscope as described before [60 (link)]. The SN pars compacta was delineated based on visual observation and morphology. Every fifth section throughout the rostro-caudal extent of the SN was analyzed, with a total of six sections for each animal. The coefficients of error, calculated according to the procedure of Schmitz and Hof as estimates of precision [61 (link)] varied between 0.07 and 0.16. The conditions of the experiment were blinded to the investigator. To determine the terminal density in the striatum pictures of TH stained sections were taken of 3 sections spaced 250 μm apart and analyzed using ImageJ. The number of P-S129 α-SYN-positive neuritic inclusions in the striatum was counted on a representative section 4 weeks after injection from the animals of the ‘high α-SYN dose’ experiment.
Van Rompuy A.S., Oliveras-Salvá M., Van der Perren A., Corti O., Van den Haute C, & Baekelandt V. (2015). Nigral overexpression of alpha-synuclein in the absence of parkin enhances alpha-synuclein phosphorylation but does not modulate dopaminergic neurodegeneration. Molecular Neurodegeneration, 10, 23.
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