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Dg10 120

Manufactured by Thorlabs
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The DG10-120 is a precision waveform generator that can produce square, triangle, and sine waveforms. It has a frequency range of 0.1 Hz to 2 MHz and an output amplitude up to 20 Vpp. The DG10-120 is designed for a variety of laboratory applications requiring accurate and flexible signal generation.

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Lab products found in correlation

G2500, by Merck Group (1 mentions) Rms4x, by Thorlabs (1 mentions) Dmlp550r, by Thorlabs (1 mentions) Ac254 150 a ml, by Thorlabs (1 mentions) Acl25416u, by Thorlabs (1 mentions) Cps532, by Thorlabs (1 mentions) H10721 20, by Hamamatsu Photonics (1 mentions)

5 protocols using dg10 120

1

Optical Scrambling and Visualization Technique

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For this proof-of-principle demonstration, we used ground glass diffusers (DG10-120, Thorlabs, USA) to scatter light. No optical focus was discernible after a single diffuser, indicating that the wavefront was sufficiently scrambled. Furthermore, neighboring pixels in the optimized phase map appeared randomized, which indicated that the light was fully scrambled by the diffusers17 (link).
An optical path to visualize the optical focus from above (shown in Fig. 1) was created by inserting a clear gelatin layer between the two diffusers. The layer was made using 10% by weight of porcine skin gelatin (G2500, Sigma-Aldrich, USA) and 90% distilled water. A gelatin bar containing fluorescent quantum dots (QSA-600-2, Ocean Nanotech, USA; conc. 0.26 μM) was embedded within the clear layer. The transducer was then positioned so that its focus overlapped the bar in both the x and z directions.
Tay J.W., Lai P., Suzuki Y, & Wang L.V. (2014). Ultrasonically encoded wavefront shaping for focusing into random media. Scientific Reports, 4, 3918.
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2

Optical Tracking Microscope Design

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The tracking microscope was built predominantly using optics and an optical cage system from Thorlabs. The basic optical path consists of a 4x NA 0.1 Olympus PLAN objective (Thorlabs #RMS4X) with a 150 mm tube lens (Thorlabs #AC254-150-A-ML), yielding a magnification of 3.33X. The image is collected on a monochrome Chameleon3 camera (FLIR, #CM3-U3-13Y3M), with a 1/2" sensor format and 1280 × 1021 pixels (4.8 μm/px at sensor plane, 1.44 μm/px at object plane). This yields a field of view of 1.84 × 1.47 mm. We illuminated the sample with a 780 nm, 18 mW IR LED (Thorlabs #LED780E) in a transillumination configuration. The LED was diffused using a ground glass diffuser (Thorlabs #DG10-120), and collimated onto the sample using a f=16 mm aspheric condenser lens (Thorlabs #ACL25416U) placed 16 mm from the diffuser surface.
The microscope path also included a 4.5 mW, 532 nm laser diode module (Thorlabs #CPS532) for optogenetic stimulation. The laser beam (3.5 mm diameter) was combined with the main optical path using a 550 nm dichroic (Thorlabs #DMLP550R), then focused on the back focal point of the objective using a f = 100 mm planoconvex lens (Thorlabs #LA1509-A-ML). This illuminated a circular region roughly 1.6 mm in the sample plane, with a calculated average intensity of 150 μW/mm2.
Cermak N., Yu S.K., Clark R., Huang Y.C., Baskoylu S.N, & Flavell S.W. (2020). Whole-organism behavioral profiling reveals a role for dopamine in state-dependent motor program coupling in C. elegans. eLife, 9, e57093.
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3

Pulsed Laser Illumination Techniques for PAT Imaging

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A tunable, 10 nanoseconds pulsed laser (Phocus Core, Optotek, Carlsbad, CA, USA) was used for all PAT imaging experiments. This laser generates around 100 mj per pulse at 532 nm. In the full-ring illumination mode, a large parabolic reflector (P19–0300, Optiforms Inc., Temecula, CA, USA) was used with a 10 mm diameter cone mirror (68–791, Edmund Optics, Barrington, NJ, USA) to create the 4 mm ring-shaped beam on the phantom surface (Figure 2a). Since the beam position is stationary, neither the cone mirror nor the parabolic reflector is mobile. The ring location was adjusted across each cross-section by translating the phantom in the vertical direction (Figure 2b). For the diffused-beam experiments, a 120 grit ground glass diffuser (DG10–120, Thorlabs Inc., Newton, NJ, USA) was placed in the laser light path inside the water tank after removing the cone mirror (Figure 2c). Finally, point illumination only uses the 45-degree mirror for directing the laser beam onto the phantom (Figure 2d).
Alijabbari N., Alshahrani S.S., Pattyn A, & Mehrmohammadi M. (2019). Photoacoustic Tomography with a Ring Ultrasound Transducer: A Comparison of Different Illumination Strategies. Applied sciences (Basel, Switzerland), 9(15), 10.3390/app9153094.
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4

Optimized Focused Light Generation

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The system diagram is shown in Fig. 3a. The illumination source was a continuous-wave 532 nm laser (Verdi V5, Coherent, Inc.), producing collimated light beam for illumination. The amplitude modulator is a digital-micromirror device (DMD) with pixel size of 1024 × 768 (Texas Instruments, Inc.). A ground glass diffuser (DG10-120, Thorlabs, Inc.) was used as scattering medium, followed by an objective lens (LA1765-A, Thorlabs, Inc.). A beam splitter (BS004, Thorlabs, Inc.) was placed after the scattering medium to divide the scattered beam and relay onto the PMT (H10721-20, Hamamatsu) and the camera (PCO.edge 5.5, PCO, Corp.), respectively. The camera was located at the conjugate position of the PMT to monitor the image of the generated focus. A number of 10 patterns was generated and displayed on the DMD with corresponding images and PMT values collected to evaluate the initial focus intensity.
Yang J., He Q., Liu L., Qu Y., Shao R., Song B, & Zhao Y. (2021). Anti-scattering light focusing by fast wavefront shaping based on multi-pixel encoded digital-micromirror device. Light, Science & Applications, 10, 149.
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5

Pulsed Laser Illumination Techniques for PAT Imaging

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A tunable, 10 nanoseconds pulsed laser (Phocus Core, Optotek, Carlsbad, CA, USA) was used for all PAT imaging experiments. This laser generates around 100 mj per pulse at 532 nm. In the full-ring illumination mode, a large parabolic reflector (P19–0300, Optiforms Inc., Temecula, CA, USA) was used with a 10 mm diameter cone mirror (68–791, Edmund Optics, Barrington, NJ, USA) to create the 4 mm ring-shaped beam on the phantom surface (Figure 2a). Since the beam position is stationary, neither the cone mirror nor the parabolic reflector is mobile. The ring location was adjusted across each cross-section by translating the phantom in the vertical direction (Figure 2b). For the diffused-beam experiments, a 120 grit ground glass diffuser (DG10–120, Thorlabs Inc., Newton, NJ, USA) was placed in the laser light path inside the water tank after removing the cone mirror (Figure 2c). Finally, point illumination only uses the 45-degree mirror for directing the laser beam onto the phantom (Figure 2d).
Alijabbari N., Alshahrani S.S., Pattyn A, & Mehrmohammadi M. (2019). Photoacoustic Tomography with a Ring Ultrasound Transducer: A Comparison of Different Illumination Strategies. Applied sciences (Basel, Switzerland), 9(15), 10.3390/app9153094.
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