Anti-HA antibody was obtained from Santa Cruz Biotechnology. Anti-HIF1α (610958) was obtained from BD Transduction Laboratories. Anti–HIF2α was from Novus Biologicals (NB100-122). Anti-glucose transporter type 1 (GLUT1) antibody was obtained from Abcam. Anti-DNMT1 (CST 5119 S) was purchased from Cell Signalling Technology. Anti- vinculin (V9264) was purchased from Sigma.
Anti vinculin v9264
Manufactured by Merck Group
Anti-vinculin (V9264) is a monoclonal antibody that binds to the protein vinculin. Vinculin is a cytoskeletal protein that plays a role in cell-cell and cell-matrix adhesion. The Anti-vinculin (V9264) antibody can be used for the detection and localization of vinculin in various applications such as Western blotting, immunohistochemistry, and immunocytochemistry.
Automatically generated - may contain errors
Lab products found in correlation
Glut1 antibody, by Abcam (1 mentions)
Nb100 122, by Novus Biologicals (1 mentions)
Anti ha antibody, by Santa Cruz Biotechnology (1 mentions)
Anti hif 2α, by Novus Biologicals (1 mentions)
Anti tubulin t5168, by Merck Group (1 mentions)
Anti ha antibody, by Cell Signaling Technology (1 mentions)
Anti flag f1804, by Merck Group (1 mentions)
Direct detect, by Merck Group (1 mentions)
Hrp linked secondary antibody, by Cell Signaling Technology (1 mentions)
Chemidoc, by Bio-Rad (1 mentions)
3 protocols using anti vinculin v9264
1
Antibody Characterization for HIF Signaling
2
Antibody Validation for Western Blot and ELISA
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Anti-HA antibody was obtained from Cell Signalling Technology (C29F4; 1:2000 dilution western blot). Anti-elongin B (11447; 1:500 dilution ELISA) and Anti-HIS (8036; 1:1000 dilution western blot) were obtained from Santa Cruz. Anti-tubulin (T5168; 1:5000 western blot), anti-vinculin (V9264, 1:10,000 dilution western blot) and anti-FLAG (F1804; 1:5000 dilution western blot; 1:1000 dilution immunoprecipitation) were obtained from Sigma-Aldrich.
3
Western Blotting Analysis of BAT Lysates
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Whole-tissue BAT lysates were prepared as previously described and quantified by Direct Detect (Millipore) (23 (link)). For blotting NCoR1/2, 50 μg protein lysate was loaded per lane, with each lane representing a separate biological replicate. Ladder used was a prestained high-range multicolor protein ladder (26625; Thermo Fisher Scientific). Wet transfer to PVDF was done overnight, and primary antibodies were applied in 5% BSA overnight, all at 4 °C. Primary antibodies used were anti-SMRTe (06-891; Millipore; 1:1,000), anti-NCoR1 (5948S; Cell Signaling Technology; 1:1,000), and anti-Vinculin (V9264; Sigma; 1:5,000), with appropriate HRP-linked secondary antibodies (Cell Signaling Technology; 1:10,000) applied at room temperature for 1 h. Blots were revealed using either film or a Bio-Rad ChemiDoc.
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