The tissue slides were incubated overnight at 4°C with antibodies specific for human nuclear antigen (1:100, Millipore, Stockholm, Sweden), human c-Met (1:100, RDI, Concord, MA, USA), human α-fetoprotein (1:200), human CK8 (1:200), human CK18 (1:200), human CK19 (1:200), human hepatocyte-specific antigen (1:100, all antibodies from AH diagnostics) and anti-p53 (1:100, antibodies-online GmbH) and anti-BER EP4 (Dako). The rest of the procedure is described by us in detail elsewhere [7 (link)]. For details, please see supplement S1 .
Anti ber ep4
Manufactured by Agilent Technologies
Sourced in United States, Denmark
The Anti-BER EP4 is a lab equipment product designed for use in scientific research and analysis. It serves as a tool for the detection and measurement of base excision repair (BER) activity. The core function of the Anti-BER EP4 is to provide researchers with a reliable and accurate method to assess BER processes in various experimental settings.
Automatically generated - may contain errors
Lab products found in correlation
Human nuclear antigen, by Merck Group (1 mentions)
Anti α smooth muscle actin, by Abcam (1 mentions)
Vectastain universal abc kit, by Vector Laboratories (1 mentions)
Anti tlr2, by Thermo Fisher Scientific (1 mentions)
Anti tlr4, by Thermo Fisher Scientific (1 mentions)
Anti desmin, by Abcam (1 mentions)
Anti vimentin, by Abcam (1 mentions)
2 protocols using anti ber ep4
1
Immunohistochemical Staining Protocol
2
Immunocytochemistry Staining of Cell Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Immunocytochemistry was undertaken as reported previously 24 (link) using Vectastain ABC Universal kit (Vector Laboratories, Peterborough, UK). Cells on coverslips were thawed, hydrated and endogenous peroxidase activity was quenched in 0·3% H2O2 in methanol for 30 min. Coverslips were incubated with the following primary antibodies (for 1 h at room temperature or overnight at 4°C): anti-BerEP4 (Dako, Glostrup, Denmark), anti-α-smooth muscle actin (abcam, Cambridge, UK), anti-vimentin (abcam), anti-desmin (abcam), anti-TLR-2 (eBioscience, San Diego, CA, USA), anti-TLR-4 (eBioscience) or anti-TLR-5 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA). Following incubation with biotinylated secondary antibody and avidin–biotin complex, peroxidase activity was developed with diaminobenzidene (DAB) solution as per the manufacturer's instructions.
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