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Sc 53623

Manufactured by R&D Systems
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Sc-53623 is a laboratory reagent product manufactured by R&D Systems. It is a small molecule that serves as a selective and potent inhibitor of a specific biological target. The core function of this product is to modulate the activity of the target in in vitro research applications.

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Lab products found in correlation

Donkey anti mouse alexa fluor 555, by Thermo Fisher Scientific (2 mentions) Alexa fluor 488 donkey anti rabbit, by Thermo Fisher Scientific (2 mentions) Mab2018, by R&D Systems (2 mentions) Jam a, by Santa Cruz Biotechnology (2 mentions) Hoechst 33342, by Thermo Fisher Scientific (2 mentions) Vectashield, by Vector Laboratories (2 mentions)

2 protocols using sc 53623

1

Immunofluorescence Staining of Cell Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were plated onto coverslips in 6 well plates, fixed with 4% paraformaldehyde, blocked in donkey serum with 0.1% Triton X-100, and then incubated with the appropriate primary antibody (SerpinB3; PA5–30164 Invitrogen, 1:500, JAM-A; Santa Cruz sc-53623, 1:500, SOX2; R&D, MAB2018) followed by a species-specific secondary antibody. Secondary antibodies were as follows, donkey-anti mouse Alexa Fluor 555 (ThermoFisher) and donkey-anti rabbit Alexa Fluor 488 (ThermoFisher). The cells were then stained with Hoechst 33,342 (Invitrogen H3570, 1:3000) before being mounted with Vectashield (Vector Labs) onto glass cover slides and imaged using a confocal microscope.
Lauko A., Volovetz J., Turaga S.M., Bayik D., Silver D.J., Mitchell K., Mulkearns-Hubert E.E., Watson D.C., Desai K., Midha M., Hao J., McCortney K., Steffens A., Naik U., Ahluwalia M.S., Bao S., Horbinski C., Yu J.S, & Lathia J.D. (2022). SerpinB3 drives cancer stem cell survival in glioblastoma. Cell reports, 40(11), 111348.
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2

Immunofluorescence Staining of Cell Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were plated onto coverslips in 6 well plates, fixed with 4% paraformaldehyde, blocked in donkey serum with 0.1% Triton X-100, and then incubated with the appropriate primary antibody (SerpinB3; PA5–30164 Invitrogen, 1:500, JAM-A; Santa Cruz sc-53623, 1:500, SOX2; R&D, MAB2018) followed by a species-specific secondary antibody. Secondary antibodies were as follows, donkey-anti mouse Alexa Fluor 555 (ThermoFisher) and donkey-anti rabbit Alexa Fluor 488 (ThermoFisher). The cells were then stained with Hoechst 33,342 (Invitrogen H3570, 1:3000) before being mounted with Vectashield (Vector Labs) onto glass cover slides and imaged using a confocal microscope.
Lauko A., Volovetz J., Turaga S.M., Bayik D., Silver D.J., Mitchell K., Mulkearns-Hubert E.E., Watson D.C., Desai K., Midha M., Hao J., McCortney K., Steffens A., Naik U., Ahluwalia M.S., Bao S., Horbinski C., Yu J.S, & Lathia J.D. (2022). SerpinB3 drives cancer stem cell survival in glioblastoma. Cell reports, 40(11), 111348.
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