N n n n tetramethyl p phenylenediamine tmpd

Cholesterol, DPPH^•, indomethacin, N,N,N′,N′-tetramethyl-p-phenylenediamine (TMPD), arachidonic acid from porcine liver, cyclooxygenase 1 from sheep, cyclooxygenase 2 human recombinant, 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH), human serum albumin (lyophilized powder, essentially fatty acid free) were purchased from Sigma–Aldrich (St. Louis, MO, USA). The probes 3-[p-(6-phenyl)-1,3,5-hexatrienyl]propionic acid (DPH-PA), Merocyanine 540 (MC540), N-phenyl-1-naphthylamine (PNA) were purchased from Molecular Probes (Eugene, OR, USA). 1-Palmitoyl-2-oleoyl phosphatidylcholine (POPC), 1-palmitoyl-2-oleoyl phosphatidylethanolamine (POPE) and 1-stearoyl-2-oleoylphosphatidylserine (SOPS) were obtained from Avanti Polar Lipids (Delfzijl, The Netherlands). Cyanidin 3-O-glucoside was obtained from Extrasynthese (Genay, France) and soya been lecithin (SPC) from Phospholipid GmbH (Köln, Germany).

2,2′-Azobis(2-amidinopropane) dihydrochloride (AAPH), human serum albumin (HSA) (lyophilized powder, essentially fatty acid free), indomethacin, N,N,N’,N’-tetramethyl-p-phenylenediamine (TMPD), arachidonic acid from porcine liver, cyclooxygenase 1 from sheep, cyclooxygenase 2 human recombinant, 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DPPC), l-α-Phosphatidylcholine (from egg yolk) were purchased from Sigma–Aldrich. The probes 1,6-diphenyl-1,3,5-hexatriene (DPH), 3-[p-(6-phenyl)-1,3,5-hexatrienyl]propionic acid (DPH-PA), Merocyanine 540 (MC540), and N,N,N-trimethyl-4-(6-phenyl-1,3,5-hexatrien-1-yl)phenylammonium p-toluenesulfonate (TMA-DPH) were purchased from Molecular Probes (Eugene, OR, USA). Tris (hydroxymethyl) aminomethane (Tris:HCl) were obtained from “Chempur” (Piekary Śląskie, Poland). Quercetin (Q) was purchased from Sigma-Aldrich. Yellow powder, ¹H-NMR (DMSO-d₆) δ_H: 6.19 (1H, d, J = 2.1 Hz, H-6), 6.41 (1H, d, J = 2.1 Hz, H-8), 6.89 (1H, d, J = 8.5 Hz, H-5′), 7.54 (1H, dd, J = 8.5, 2.2 Hz, H-6′), 7.68 (1H, d, J = 2.2 Hz, H-2′). ¹³C-NMR (DMSO-d₆) δ_C: 93.2 (C-8), 98.0 (C-6), 102.9 (C-10), 114.9 (C-2′), 115.4 (C-5′), 119.8 (C-6′), 121.8 (C-1′), 135.6 (C-3), 144.9 (C-3′), 146.6 (C-2), 147.5 (C-4′), 156.0 (C-9), 160.6 (C-5), 163.7 (C-7), 175.7 (C=O).

Phenylmethylsulfonyl fluoride (PMSF), KH₂PO₄, NaCl, ethylenediamine tetraacetic acid (EDTA), ATP, ADP, MgCl_2, β-hydroxybutyrate, phosphoenolpyruvate, sodium L-lactate, sodium pyruvate, imidazole, hydrazine, aldolase, triosephosphate isomerase, glyceraldehyde 3-phosphate dehydrogenase, fructose 6-phosphate, glucose 6-phosphate, glucose, G6PDH, КСl, mannitol, bovine serum albumin, glutamate, malate, rotenone, succinate, antimycin A, ascorbate, N,N,N′,N′-tetramethyl-p-phenylenediamine (TMPD), and NaN₃ were purchased from Sigma-Aldrich (St. Louis, MO, USA); Tris-HCl, NADP, NADH, NAD⁺, glycine, lactate dehydrogenase were from Carl Roth (Karlsruhe, Germany); diagnostic kits for determination of glucose and triacylglycerides were from Felicit Diagnostics Ltd. (Dnipro, Ukraine). All other reagents were obtained from local suppliers (Ukraine) and were of analytical grade.