Hypersil gold c18 analytical column

DA and 5-HT contents in the dialysate fractions were analyzed by HPLC with electrochemical detection. Chromatography was performed using an Ultimate 3000 System (Dionex, Sunnyvale, CA, USA), a Coulochem III electrochemical detector (model 5300; ESA, Chelmsford, MA, USA) with 5020 guard cell, 5014B microdialysis cell, and Hypersil Gold-C18 analytical column (3 × 100 mm; Thermo Scientific, Waltham, MA, USA). The mobile phase was composed of 0.1 M potassium phosphate buffer adjusted to pH 3.6, 0.5 mM EDTA, 16 mg/L 1-octanesulfonic acid sodium salt, and 2% methanol. The flow rate during analysis was set at 0.7 mL/min. The applied potential was + 600 mV for the guard cell, and E1 = − 50 mV and E2 = + 300 mV for the microdialysis cells, with a sensitivity set at 50 nA/V. The chromatographic data was processed by Chromeleon v. 6.80 (Dionex) software, run on a personal computer.

Extracellular DA and 5-HT levels were analyzed using an Ultimate 3000 System (Dionex, Sunnyvale, CA, USA), electrochemical detector Coulochem III (model 5300; ESA, Chelmsford, MA, USA) with a 5020 guard cell, a 5040 amperometric cell and a Hypersil Gold C18 analytical column (3 μm, 100 × 3 mm; Thermo Fisher Scientific, Sunnyvale, CA, USA). The details of the method have been described elsewhere [29 (link),69 (link)]. The chromatographic data were processed by the Chromeleon v.6.80 (Dionex, Sunnyvale, CA, USA) software package run on a personal computer. The limit of detection of DA and 5-HT in dialysates was 0.002 pg/10 μL for DA and 0.01 pg/10 μL for 5-HT.
Glutamate and GABA levels in the extracellular fluid were measured by HPLC with electrochemical detection after the derivatization of samples with OPA/sulfite reagent to form isoindole-sulfonate derivatives, as previously described [29 (link),69 (link)]. The data were processed using Chromax 2005 (Pol-Lab, Warszawa, Poland) software on a personal computer. The limit of detection of glutamate and GABA in dialysates was 0.03 ng/10 μL and 6.4 pg/10 μL, respectively.

DA and 5-HT contents in the dialysate fractions were analyzed by HPLC with electrochemical detection. Chromatography was performed using an Ultimate 3000 System (Dionex, Sunnyvale, CA, USA), a Coulochem III electrochemical detector (model 5300; ESA, Chelmsford, MA, USA) with a 5020 guard cell, a 5014B microdialysis cell and a Hypersil Gold-C18 analytical column (3 × 100 mm; Thermo Scientific, Waltham, MA, USA). The mobile phase was composed of 0.1 M potassium phosphate buffer adjusted to pH 3.6, 0.5 mM EDTA, 16 mg/L 1-octanesulfonic acid sodium salt, and 2% methanol. The flow rate during analysis was set at 0.7 mL/min. The applied potential of a guard cell was + 600 mV, while those of microdialysis cells were: E₁ = − 50 mV and E₂ = + 300 mV with a sensitivity set at 50 nA/V. The chromatographic data was processed by Chromeleon v. 6.80 (Dionex) software, run on a personal computer.