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5 protocols using phb pellets

1

Bioplastic Production Protocol

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All chemicals used in this study were of analytical grade. Solvents used in this study, including chloroform and dichloromethane (DCM), were obtained from Sigma-Aldrich(MA, USA). PHB pellets were obtained from Goodfellow (Huntingdon, UK), and other bioplastic pellets and N-lauroylsarcosine sodium salt (Sarkosyl NL) were purchased from Sigma-Aldrich (MA, USA). Carbon and nitrogen sources were obtained from Junsei (Tokyo, Japan).
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2

Biopolymer Synthesis: Reagents & Protocols

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All chemicals used in this study were of analytical grade. Chloroform, acetonitrile, poly-3-hydroxybutyrate-co-3-hydroxy-valerate(P(3HB-co-3HV)) pellets, p-nitrophenyl acetate, butyrate, octanoate, decanoate, and dodecanoate were obtained (Sigma-Aldrich, St. Louis, MO, USA). We obtained p-nitrophenyl hexanoate (Tokyo Chemical Industry, Tokyo, Japan). PHB pellets were obtained(Goodfellow Cambridge Ltd., Huntingdon, UK). PBAT and PBS pellets were obtained (Gio Soltech Co., Ltd. Wonju, Korea). Poly-3-hydroxybutyrate-co-4-hydroxybutyrate (P(3HB-co-4HB)) pellets were obtained (CJ, Suwon, Korea). Dichloromethane (DCM), fructose, and sucrose were obtained (Junsei Chemical Co., Tokyo, Japan). Glucose and xylose were obtained (Duksan Pure Chemicals, Ansan, Korea). Galactose was obtained (Daejung Chemicals, Siheung, Korea).
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3

Bioplastic Synthesis and Characterization

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All chemicals used in this study were of analytical grade. Solvents used in making plates and film such as chloroform and dichloromethane (DCM) were obtained from Sigma-Aldrich (USA). Sodium dodecyl sulfate (SDS) was purchased from Biosesang (Korea). PHB pellets were obtained from Goodfellow (UK) and other bioplastic pellets and sodium 3-hydroxybutyrate (3HB) were purchased from Sigma-Aldrich. Carbon and nitrogen sources were purchased from Junsei (Japan).
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4

Chloroform-based PHB Characterization

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All chemicals used in this study were of analytical grade. The chloroform, PHB powder, and PHB films used for the plates and degradation experiments were purchased from Sigma-Aldrich (USA). The PHB pellets used for gel permeation chromatography (GPC) analysis were purchased from Goodfellow (UK).
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5

Solvent-based PHB Polymer Extraction

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All chemicals used in this study were of analytical grade. PHB pellets (Average MW: 550,000 g/mol) were obtained from Goodfellow Cambridge Ltd. (Huntingdon, UK). Chloroform and ethanol were obtained from Fisher Scientific (Hampton, NH, USA). Bis(2-ethylhexyl) adipate, bis(2-ethylhexyl) sebacate, lauric acid, triacetin, tributyl citrate, tributyl 2-acetylcitrate, l-Linalool, and geraniol were obtained from Sigma-Aldrich (St. Louis, MO, USA).
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