Sars cov 2 spike rbd wh 01 protein

For mouse studies, vaccines consisted of 10 µg SARS-CoV-2 Spike RBD WH-01 protein (GenScript, cat# Z03483), combined with 1 nmol AMP-CpG-7909 (AMP-CpG, Avecia), or soluble CpG 7909 (soluble CpG, InvivoGen, tlrl-2006,). Mock treatment groups received a matching dose of adjuvant in the absence of antigen or were treated with vehicle alone (phosphate-buffered saline, PBS), as indicated. Antigen rechallenge doses consisted of 10 µg SARS-CoV-2 Spike RBD WH-01 protein, administered subcutaneously bilaterally into the tail base or intranasally. For experiments testing storage temperature conditions, doses were admixed and stored at 4 °C or room temperature (22 °C) for the indicated time. For NHP studies, vaccines consisted of either SARS-CoV-2 Spike RBD WH-01 protein (GenScript; cat# Z03483) or SARS-CoV-2 WH-01 Spike protein (Acro Biosystems, SPN-C5H9) at 140 µg admixed with AMP-CpG at either 5 or 10 mg per dose.