Organoids were fixed in 4% paraformaldehyde solution and embedded in paraffin. Sections were subjected to H&E and IF staining. The following primary antibodies were used for IF staining: KRT19 (ab7755, Abcam; 1:100), CYP3A5 (ab108624, Abcam; 1:200), KRT17 (sc393002, Santa Cruz; 1:50), GATA6 (AF1700, R&D; 1:100), Ki-67 (ab16667, Abcam; 1:200) and DAPI (D9542, Sigma; 1:1,000). Images of H&E and IF staining were acquired using imaging system Tissue-FAXS software (TissueGnostics). H&E images were acquired using a ×20 objective lens in brightfield. IF images were acquired using a ×20 objective lens with light-emitting diodes (LEDs) with specific light filters. IF images of negative-control sections were used to set the appropriate gating to exclude background IF and non-specific binding signals. The expression level of each protein was calculated by the percentage of protein-positive-stained cells in DAPI-positive cells.
Ab108624
Manufactured by Abcam
Sourced in United States
Ab108624 is a lab equipment product from Abcam. It serves as a core function, but a detailed description cannot be provided while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
Lab products found in correlation
Ab7755, by Abcam (1 mentions)
Ab16667, by Abcam (1 mentions)
Af1700, by R&D Systems (1 mentions)
D9542, by Merck Group (1 mentions)
Sc 393002, by Santa Cruz Biotechnology (1 mentions)
Tissuefaxs software, by TissueGnostics (1 mentions)
Ab33586, by Abcam (1 mentions)
Ab135813, by Abcam (1 mentions)
Ab172730, by Abcam (1 mentions)
3 protocols using ab108624
1
Immunofluorescence Staining of Organoid Cultures
2
Cytochrome P450 Antibody Validation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ITE was a gift from Dr Jiasheng Song, (AhR Pharmaceuticals, Inc., Madison, WI, USA). Anti-CYP450 isoenzyme 1A1 antibody (ab126828), anti-CYP450 isoenzyme 1B1 antibody (ab33586), anti-CYP450 isoenzyme 1A2 antibody (ab56073), CYP3A4 antibody (ab135813), CYP3A5 antibody (ab108624), CYP2D6 antibody (ab62204), CYP2C9 antibody (ab150364) and CYP2E1 antibody (ab90564) were all purchased from Abcam (Cambridge, MA, USA).
3
IHC Staining of Human OSCC Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
IHC staining of human OSCC tissues was performed on deparaffinized OSCC tissue sections using primary antibody against CYP3A5 (dilution, 1:100; cat. no. ab108624; Abcam) overnight at 4°C. For the negative control for immunohistochemistry analysis, the primary antibody was replaced with normal IgG (dilution 1:100; cat. no. ab172730; Abcam) overnight at 4°C. The slides were subsequently treated with biotinylated anti-rabbit secondary antibody anti-rabbit IgG H&L (HRP) (dilution 1:4,000; cat. no. ab205718; Abcam) and counterstained with hematoxylin. Control experiments were performed using non-immune immunoglobulins as opposed to specific antibody. Immunostained images were captured using a digital camera; five images were captured at random.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!