Detection of seizure activity was based on continuous video/EEG-monitoring with the use of infrared light sensitive cameras, a multiple-channel PCI anolog-digital converter (ABUS Security-Tech, Affing, Germany), 1-channel bioamplifiers (BioAmps, AD Instruments, Hastings, East Sussex, United Kingdom), and analog-digital converters (PowerLab/800s, AD Instruments, Hastings, East Sussex, United Kingdom). Video- and EEG-recordings were analyzed using the Digi-Protect Searcher 6.275 beta (ABUS Security-Tech, Affing, Germany) and the Chart5 for windows software (AD Instruments, Hastings, East Sussex, United Kingdom). Two and four weeks following SE induction, continuous video/EEG monitoring was performed during two days prior to PET scanning. Eight to nine weeks following SE induction spontaneous seizure activity was assessed by continuous video/EEG monitoring during two weeks prior to PET scanning. The total number of generalized tonic-clonic motor seizures was recorded including information about the seizure stage (4 or 5). Electrode-implanted control animals were also transiently placed in the monitoring cages and handled in parallel. In addition, seizures observed by video- and EEG-recordings, seizures observed during handling or by direct observation of the animals in their home cages were noted during the entire study.
Chart5 for windows software
Manufactured by ADInstruments
Sourced in Germany, United States
Chart5 for Windows is a data acquisition and analysis software that enables users to record, display, and analyze experimental data. It provides a comprehensive set of tools for real-time data acquisition, visualization, and post-processing. The software supports a wide range of data sources and file formats, allowing users to seamlessly integrate it into their research workflows.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using chart5 for windows software
1
Continuous Video-EEG Monitoring of Seizures
2
Aortic Contractility and Endothelial Function
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After collecting the blood, the thoracic aortas were isolated immediately. The fat and connective tissue were carefully excised, and the thoracic aortas were cut into rings about 3 mm long and hung on stainless steel hooklets in improved Krebs solution bubbled with 95% O 2 and 5% CO 2 at 37°C. Rings were connected to transducers and the Powerlab system (AD instruments, USA). The rings were equilibrated for 2 h and the Krebs solution was changed every 30 min. The contraction signals were recorded by the Powerlab system and analyzed with the Chart 5 for Windows software (AD instruments, USA). The rings were soaked in 5 mL Krebs solution supplemented with norepinephrine (10 -6 M). When the vasoconstriction curves reached the plateau phase, acetylcholine (10 -10 -10 -6 M), A23187 (10 -5 M) and sodium nitroprusside (10 - 9 -10 -6 M) were added to observe the endothelium-dependent and independent relaxation of aortic rings respectively. The contraction tension was recorded and relaxation was calculated as the percent reduction from norepinephrine-induced tension.
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