Adenovirus expressing HMGCS2 (−231/+57) luciferase (AdHMGCS2-Luc) was generated using VectorBuilder (Guangzhou, China). AdHMGCS2-Luc (1 × 109 pfu/kg of body weight) was injected into the tail veins of Pak4 LKO or WT mice, followed by fasting for 24 h or KD-feeding for 3 days. Thereafter, mice were injected with 150 mg/kg of firefly d ‐luciferin (#LUCK-100, GoldBio, St Louis, MO, USA), anesthetized after 10 min, and imaged using the IVIS Luminar XR Imaging System (Caliper Life Sciences, Hopkinton, MA, USA).
Ivis luminar xr imaging system
Manufactured by PerkinElmer
Sourced in United States
The IVIS Luminar XR Imaging System is a high-performance in vivo imaging platform designed for preclinical research. It provides researchers with a non-invasive tool to visualize and quantify bioluminescent and fluorescent signals in living small animal models. The system utilizes advanced optics, sensitive detectors, and a temperature-controlled imaging chamber to capture highly sensitive and reproducible images of biological processes.
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Lab products found in correlation
5 protocols using ivis luminar xr imaging system
1
HMGCS2 Regulation in Fasting and Keto
2
Adenovirus-Mediated Sirt6 Modulation in Mice
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Adenovirus expressing G6Pase (-231/+57) luciferase (Ad-G6Pase-Luc) was kindly provided by Dr. Um SH (Sungkyunkwan University, Suwon, Korea). Ad-G6Pase-Luc (1 × 109 pfu/kg of body weight) was injected into tail veins of mice. The following day, Sirt6 adenoviruses were intravenously injected into mice, followed by statin treatment for 3 consecutive days. After the last dosing of statin, 6 h-fasted mice were injected with 150 mg/kg of firefly D‐luciferin (GoldBio, St Louis, MO, USA). After 10 min, mice were anesthetized and imaged using the IVIS Luminar XR Imaging System (Caliper Life Sciences, Hopkinton, MA, USA).
3
HMGCS2 Regulation in Fasting and Keto
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Adenovirus expressing HMGCS2 (−231/+57) luciferase (AdHMGCS2-Luc) was generated using VectorBuilder (Guangzhou, China). AdHMGCS2-Luc (1 × 109 pfu/kg of body weight) was injected into the tail veins of Pak4 LKO or WT mice, followed by fasting for 24 h or KD-feeding for 3 days. Thereafter, mice were injected with 150 mg/kg of firefly d ‐luciferin (#LUCK-100, GoldBio, St Louis, MO, USA), anesthetized after 10 min, and imaged using the IVIS Luminar XR Imaging System (Caliper Life Sciences, Hopkinton, MA, USA).
4
Adenoviral-mediated Glucagon-Stimulated Bioluminescence
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Seven-week-old male C57BL/6 J mice were intravenously injected with 1 × 109 p.f.u of CRE luciferase adenovirus (db/db male mice, 5 × 1010 p.f.u) from Vector Biolabs. After 3 days, 6 h-fasted mice were injected intraperitoneally with 100 μg/kg glucagon 1 h before imaging. Then, the mice were injected intraperitoneally with 150 mg/kg firefly d ‐luciferin (LUCK-100, GoldBio, St Louis, MO, USA). After 10 min, mice were anesthetized and imaged at 15 min using the IVIS Luminar XR Imaging System (Caliper Life Sciences, Hopkinton, MA, USA).
5
HMGCS2 Regulation in Fasting and Keto
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Adenovirus expressing HMGCS2 (−231/+57) luciferase (AdHMGCS2-Luc) was generated using VectorBuilder (Guangzhou, China). AdHMGCS2-Luc (1 × 109 pfu/kg of body weight) was injected into the tail veins of Pak4 LKO or WT mice, followed by fasting for 24 h or KD-feeding for 3 days. Thereafter, mice were injected with 150 mg/kg of firefly d ‐luciferin (#LUCK-100, GoldBio, St Louis, MO, USA), anesthetized after 10 min, and imaged using the IVIS Luminar XR Imaging System (Caliper Life Sciences, Hopkinton, MA, USA).
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