HUSMCs (cc-2562; Lonza, Basel, Switzerland) were maintained on tissue culture plastic in HUSMC complete media (SmGM-2 medium [cc-3181; Lonza] supplemented with 5% fetal bovine serum, growth factors [cc-4149; Lonza]), 100 U/mL penicillin, and 100 mg/mL streptomycin. Cells in passages 3–8 were used for all experiments. HUSMCs in six-well plates were transfected with ADAMTS9 siRNA (SASI_Hs02_00371041; Sigma-Aldrich), VCAN siRNA (Silencer select s229335; Ambion, Waltham, MA), or control siRNA (SIC001; Sigma-Aldrich) using Lipofectamine RNAiMAX (13778; Invitrogen, Carlsbad, CA) upon achieving 60% confluence and harvested 48 hr after transfection. For rescue experiments, ADAMTS4 or ADAMTS5 at 0.1 U/mL (Vankemmelbeke et al., 2003 (link)) was added at the time of transfection and at 24 hr or cells were plated on fibronectin (Supplemental Experimental Procedures ).
Adamts9 sirna sasi hs02 00371041
Manufactured by Merck Group
ADAMTS9 siRNA (SASI_Hs02_00371041) is a short interfering RNA (siRNA) designed to target the ADAMTS9 gene in human cells. ADAMTS9 is a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) family of proteins, which are involved in various biological processes. The ADAMTS9 siRNA can be used to study the function of the ADAMTS9 gene and its role in cellular processes.
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2 protocols using adamts9 sirna sasi hs02 00371041
1
Regulation of ADAMTS9 and VCAN by siRNA
2
Regulation of ADAMTS9 and VCAN by siRNA
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HUSMCs (cc-2562; Lonza, Basel, Switzerland) were maintained on tissue culture plastic in HUSMC complete media (SmGM-2 medium [cc-3181; Lonza] supplemented with 5% fetal bovine serum, growth factors [cc-4149; Lonza]), 100 U/mL penicillin, and 100 mg/mL streptomycin. Cells in passages 3–8 were used for all experiments. HUSMCs in six-well plates were transfected with ADAMTS9 siRNA (SASI_Hs02_00371041; Sigma-Aldrich), VCAN siRNA (Silencer select s229335; Ambion, Waltham, MA), or control siRNA (SIC001; Sigma-Aldrich) using Lipofectamine RNAiMAX (13778; Invitrogen, Carlsbad, CA) upon achieving 60% confluence and harvested 48 hr after transfection. For rescue experiments, ADAMTS4 or ADAMTS5 at 0.1 U/mL (Vankemmelbeke et al., 2003 (link)) was added at the time of transfection and at 24 hr or cells were plated on fibronectin (Supplemental Experimental Procedures ).
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