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3 protocols using cholesterol assay kit

1

Comprehensive Biochemical Assay Protocol

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Cholesterol, cholic acid, human thrombin (EC: 3.4.21.5), human fibrinogen, O-Phthaldialdehyde reagent (OPA), pepsin (EC: 3.4.23.1), pancreatin (EC: 232-468-9), bile salts, α-amylase (EC: 3.2.1.1), lysozyme (EC: 3.2.1.17), sodium taurocholate, bile assay kit, heparin sodium salt, mucin, bovine serum albumin (BSA), glucoronic acid, trichloroacetic acid, L-leucine, cholestyramine, linoleic acid, trichloroacetic acid, galactose and glucosamine were purchased from Sigma-Aldrich Chemicals Co. (St. Louis, MO, USA). M17 broth, lactose and dextrose were purchased from DIFCO (Sparks, MD, USA). Thrombin time and pro-thrombin time kits were purchased from Wiener Lab (Rosario, Argentina). A Cholesterol assay kit was purchased from RANDOX Laboratories (Crumlin, UK). The DC Lowry protein assay was purchased from Bio-Rad Laboratories (Hercules, CA, USA).
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2

Enzyme Extraction and Characterization

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ACE (EC 3.4.15.1) from rabbit lung powder, human thrombin (EC: 3.4.21.5), cholesterol, α-amylase (EC: 3.2.1.1), lysozyme (EC: 3.2.1.17), pancreatin (EC: 232-468-9), pepsin (EC: 3.4.23.1), bile salts, bovine serum albumin (BSA), galactose, glucosamine, glucuronic acid, Hippuryl-L-histidyl-L-leucine, human fibrinogen, L-leucine, mucin, O-Phthaldialdehyde reagent (OPA), cholestyramine, linoleic acid, phosphatidylcholine, sodium taurocholate and trichloroacetic acid were purchased from Sigma-Aldrich Chemicals Co. (St. Louis, MO, USA). De Man, Rogosa and Sharpe Broth (MRS) BD were purchased from Difco™ (Le Pont de Claix, Francia). cholesterol assay kit was purchased from RANDOX Laboratories (Crumlin, UK) and DC Lowry protein assay was purchased from Bio-Rad Laboratories (Hercules, CA, USA).
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3

Cholesterol and Triglyceride Determination

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The cholesterol assay kit was purchased from Randox Laboratories Co., Ltd. (Antrim, UK). The E.Z.N.A™ tissue RNA kit was from Omega Bio-Tek (Norcross, GA, USA). The high-capacity RNA-to-cDNA kit and the power SYBR® Green RT-PCR reagent kit were obtained from Applied Biosystems (Richmond, CA, USA). The TG-GPO liquid reagent set was obtained from Teco Diagnostics (Anaheim, CA, USA).
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