PET/CT scans were performed on a Biograph 64 system (Siemens Healthineers, Erlangen, Germany) with a 22.1-cm axial field of view. The patients were required to fast for at least 6 h before imaging, and serum glucose levels were kept lower than 7.4 mmol/l. Images were captured ~ 60 min after intravenous administration of 3.7 MBq of FDG per kilogram of body weight. CT was performed under the following conditions: 120 kV, 100–200 mA (adjusted by auto mA). PET images were acquired for 2.5 min per bed position from the skull base to the midthighs and reconstructed at 200 × 200 pixels using a Gaussian filter of 5.0 mm full width at half maximum value. All image reconstructions were performed with the ordered subset expectation–maximization algorithm, incorporating a CT-based transmission map.
Biograph 64 system
Manufactured by Siemens
Sourced in Germany, United States
The Biograph 64 system is a positron emission tomography (PET) and computed tomography (CT) imaging device manufactured by Siemens. It combines PET and CT technology to provide high-quality, multimodal imaging capabilities for medical applications.
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7 protocols using biograph 64 system
1
FDG PET/CT Imaging Protocol for Oncology
2
Standardized PET/CT Imaging Protocol
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18F-FDG PET/CT scan was performed on a Biograph 64 system (Siemens Healthineers, Erlangen, Germany) with a 21.6 cm axial field of view. Patients were required to fast for at least 6 h prior to imaging, and serum glucose levels were kept less than 7.4 mmol/l. Images were obtained about 60 min after intravenous administration of 3.7 MBq of 18F-FDG per kilogram of body weight. Six or seven bed positions from the base of the skull to the mid-thighs were captured. PET images were acquired for 2.5 min per bed position. CT was performed on the same scanner without contrast administration, and CT scan data were collected under the following conditions: 120 kV, 101 mA (adjusted by auto mA) and a gantry rotation speed of 0.5 s. All the CT scans were conducted via 5-mm-thick axial slices. PET images were reconstructed at 200 × 200 pixels using a Gaussian filter of 5.0 mm full width at half maximum value. All image reconstructions were performed with the ordered-subset expectation maximization algorithm, incorporating a CT-based transmission map.
3
Detecting Metastatic Melanoma via PET/CT
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Spontaneous melanoma miniswine (4 to 16 kg, Sinclair Research Center) were peritumorally injected with 124I-cRGDY-PEG-CW800-C′ dots (dose range, 19 to 28 MBq) for detecting and localizing metastatic disease using whole-body PET/CT imaging. PET/CT miniswine imaging was performed using a clinical Biograph 64 system (Siemens Medical Solutions USA, Knoxville, TN) at about 60 min following peritumoral injection of 3.6 mCi of 124I-labeled cRGDY-functionalized nanoparticles. CT scanning was performed using an effective milliampere seconds (mAs) of 100, x-ray tube voltage of 120 kilovoltage, pitch of 0.55, rotation time of 1.0 s, and scan time of 17.5 s, yielding 3-mm-thick transverse slices. PET scanning was performed over five bed positions at 2 min per bed position, and images were reconstructed using Siemens’ proprietary TrueX iterative algorithm (21 subsets, 10 iterations) in a 512 × 512 matrix and corrected for scatter and attenuation. The sites of the SLNs were then marked (based on the PET/CT scans), shaved, and prepped.
4
Standardized PET/CT Imaging Protocol
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PET/CT examinations were performed using a Biograph 16 HR (Siemens Healthineers, Erlangen, Germany) or a Biograph 64 system (Siemens Healthineers, Erlangen, Germany). All patients were required to fast and avoid strenuous exercise at least 6 hours before 18F-FDG injection, and the level of fasting blood glucose was no more than 11.0 mmol/L. Six or seven-bed positions were imaged from the base of the skull to the mid-thigh at approximately 60±5 minutes after intravenous injection of 3.7–7.4 MBq of 18F-FDG per kilogram of body weight. PET images were acquired for 2–3 minutes per bed position. The ordered-subset expectation maximization algorithm was used for all image reconstructions, incorporating a CT-based transmission map.
5
Florbetapir Amyloid PET Imaging Protocol
6
PET/CT Protocol for 2-[18F]FDG Imaging
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After patients had fasted for at least 6 hours, their blood glucose levels were confirmed to be lower than 11.1 mmol/L before 2-[18F]FDG (3.5–4.5 MBq/kg) was administered. Sixty minutes after the injection, all patients underwent 2-[18F]FDG PET/CT performed with the Discovery 710 system (GE HealthCare, Chicago, IL, USA) or the Biograph 64 system (Siemens Healthineers, Erlangen, Germany). Whole-body CT images were obtained for attenuation correction using automatic dose modulation under the following settings: voltage, 120–140 kV; current, 100 mAs; rotation, 0.8; slice thickness, 3–5 mm; and pitch, 1 (19 (link)). Whole-body PET scans were obtained from the base of the skull to the upper femur in the free-breathing mode. The parameters of PET were three-dimensional mode, 2–2.5 min/bed (30% overlap), 4–5 beds/person, 3 iterations, 21 subsets, and a Gaussian filter half-height width of 4.0 mm.
7
PET/CT Imaging Protocol for 18F-FDG
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PET/CT scans were performed using a Biograph 16 HR (Siemens Healthineers, Erlangen, Germany) or a Biograph 64 system (Siemens Healthineers, Erlangen, Germany). All patients were inquired to fast and avoid strenuous exercise at least 6 h before 18F-FDG injection, and the level of fasting blood glucose was no more than 7.0 mmol/L. Images were acquired approximately 60 ± 5 min after intravenous injection of 3.7 MBq of 18F-FDG per kilogram of body weight. Six or seven-bed positions were imaged from the base of the skull to the mid-thigh. PET images were obtained for 2–3 min per bed position. All image reconstructions were performed with the ordered-subset expectation maximization algorithm, incorporating a CT-based transmission map. PET images were reconstructed at 200 × 200 pixels using a Gaussian filter of 5.0 mm full width at half maximum value.
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