Phadia 1000

Manufactured by Thermo Fisher Scientific

Sourced in Sweden, United States

The Phadia 1000 is an automated clinical diagnostic instrument designed for in-vitro allergy testing. It utilizes fluorescence enzyme immunoassay (FEIA) technology to measure specific IgE antibodies in patient samples. The Phadia 1000 is capable of processing multiple samples simultaneously and provides quantitative results for a range of allergens.

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Lab products found in correlation

Phadia 250, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

ImmunoCAP Phadia 1000 (4 citations)

6 protocols using phadia 1000

Aspergillus Immunoglobulin Monitoring in Transplant

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Blood samples with IgG/IgE measurement were collected during the pre-transplant work-up prior to listing for transplant, and at every annual post-transplant check-up, taking place between September 2017 and January 2021. In these samples total protein level, specific IgG’s, total IgE, and specific IgE’s were measured using ImmunoCAP fluoroenzyme immunoassay, with a Phadia™ 250 instrument for IgG measurements and a Phadia™ 1000 instrument for IgE measurements (ThermoFisher, Waltham, Massachusetts, United States), as per institutional standard operating procedures.
Specific IgG’s against Aspergillus fumigatus (Gm3) and Aspergillus flavus (Gm228) were determined. For the specific IgE’s, the following were assessed: Aspergillus fumigatus (m3), Aspergillus flavus (m228) and Aspergillus niger (m207).
For specific IgG’s the lower limit of detection was 2.0 mg/L, values >50.0 mg/L for Aspergillus fumigatus and Aspergillus flavus were considered positive per assay protocol. The lower limit of detection for the specific IgE’s was 0.10 kU/L, and every detectable value was considered positive.

Beeckmans H., Van Roy E., Kaes J., Sacreas A., Geudens V., Vermaut A., Willems L., Jin X., Bos S., Vanstapel A., Van Slambrouck J., Orlitova M., Vanaudenaerde B., Ceulemans L.J., Van Raemdonck D., Neyrinck A.P., Godinas L., Dupont L.J., Verleden G.M, & Vos R. (2023). Aspergillus-Specific IgG Antibodies are Associated With Fungal-Related Complications and Chronic Lung Allograft Dysfunction After Lung Transplantation. Transplant International, 36, 10768.

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Comprehensive Allergen Sensitization Assessment

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The number of eosinophils was measured via a blood test. The Phadia 1000 was used to detect allergen sensitization (Thermo Fisher Scientific Inc., USA), based on specific IgE and serum total IgE levels. IgE levels specific to allergens, including house dust mites, dog dander, egg whites, cow milk, wheat, shrimp, the German cockroach, and the Alternaria alter were measured.

Luo J.Y., Chen H.A., Ma J., Xiao Y.X., Yao J.J., Liang J.M., Du Y.S., Wang F, & Sun B.Q. (2021). Clinical application of fractional exhaled nitric oxide and nasal nitric oxide levels for the assess eosinophilic inflammation of allergic rhinitis among children. Translational Pediatrics, 10(4), 746-753.

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Serum-specific IgE Measurement for Timothy Grass and Mugwort

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Serum-specific IgE (sIgE) against timothy grass (g6), its components Phl p 1 (g205), Phl p 2 (g206), Phl p 5 (g215), Phl p 6 (g209), Phl p 7 (g210), and Phl p 12 (g212), and mugwort (w6) were tested by the ImmunoCAP allergen system (Phadia 1000, ThermoFisher Scientific, Uppsala, Sweden). A positive value was defined as detectable sIgE ≥ 0.35kU_A/L.

Xu Y., Guan K., Sha L., Zhang J., Niu Y., Yin J, & Wang L. (2021). Sensitization Profiles of Timothy Grass Pollen in Northern China. Journal of Asthma and Allergy, 14, 1431-1439.

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Serum IgE Levels Analysis

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Patient samples were obtained from the serum bank of the AIR-SKLRD. Around 5 mL of venous blood was taken and centrifuged at 3000 g for 10 minutes to prepare the supernatant, then stored at −80°C before detection. In this study, the Phadiatop sIgE (including fungi, pollen, insects, and dust mites) and tIgE levels of serum samples were assessed using the Phadia 1000 automatic allergy analyzer (ThermoFisher, Uppsala, Sweden) for in vitro diagnostic use. Samples with a Phadiatop sIgE concentration ≥0.35 kU/L were considered positive.

Hu H., Cai C., Xue M., Luo J., Liao C., Huang H, & Sun B. (2020). Increased MMP8 Levels in Atopic Chronic Obstructive Pulmonary Disease: A Study Testing Multiple Immune Factors in Atopic and Non-Atopic Patients. International Journal of Chronic Obstructive Pulmonary Disease, 15, 1839-1848.

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Allergic Asthma Serum Biomarkers Study

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In this prospective study, we used the sera of 30 patient’s with allergic asthma that were randomly obtained from the serum bank of the Allergy Information Repository from January 2017 to December 2018 in Guangzhou, China. The samples were selected based on the following inclusion criteria: (1) patients who had clinical symptoms of allergy, such as wheezing, dyspnea, and/or chronic cough, that were not caused by a cold, sneezing, runny nose, nasal obstruction, or nasal itching; (2) patients who were positive for at least one of the allergen serum sIgE, including D. pteronyssinus, F. domesticus, B. germanica, and A. vulgaris on an assay conducted on the Phadia 1000 (≥0.35 kU/L; ThermoFisher, Sweden); and (3) no history or current use of specific allergen immunotherapy, with no autoimmune diseases, parasitic infections, or cancer. The diagnosis of asthma was recorded based on the Global Initiative for Asthma^{6 (link)} criteria that were evaluated by a respiratory specialist.

Hu H., Huang Z., Luo W., Zou X., Chen H., Liao C, & Sun B. (2021). Allergen diagnosis strategy: An experimental application of different methods in Guangzhou, Southern China. Science Progress, 104(1), 0036850421997277.

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Serum-Based Allergy Biomarkers in CRD

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Patient samples were obtained from the serum bank of the AIR-SKLRD. Within 2 days after the patients' first presentation and before they received any prescriptions, 5 mL venous blood was taken and centrifuged at 3000 g for 10 minutes to prepare the supernatant. It was then stored at -80°C. In this study, the Phadiatop sIgE (including fungi, pollens, insects and dust mites) and tIgE levels of the serum samples of the 252 patients with CRD were tested with the Phadia 1000 automatic allergy analyzer (ThermoFisher, Uppsala, Sweden) for in vitro diagnostic use. Samples with a Phadiatop sIgE concentration ≥ 0.35 kU/L were positive.

Hu H., Huang H., Zheng P., Li L., Cai C., Li N, & Sun B. (2021). The sensitization characteristics of adult Chinese patients diagnosed with chronic respiratory diseases. Asian Pacific journal of allergy and immunology, 39(2).

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