Uv 1601 spectrophotometer

Manufactured by Shimadzu

Sourced in Japan, Italy, Germany, Austria

The UV-1601 spectrophotometer is a compact and versatile instrument designed for UV-visible spectroscopic analysis. It offers a wavelength range of 190 to 1,100 nanometers and can measure absorbance, transmittance, and reflectance. The UV-1601 features a high-resolution display, intuitive user interface, and data storage capabilities.

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Spelling variants of this product

UV-1601 (459 citations) UV spectrophotometer (197 citations) UV-1601 PC spectrophotometer (45 citations) UV-Vis 1601 spectrophotometer (32 citations) UV-1601 UV-VIS spectrophotometer (27 citations) Model UV-1601 spectrophotometer (6 citations) 1601 spectrophotometer (5 citations) 1601 PC UV-visible Spectrophotometer (5 citations) UV-1601 dual-beam spectrophotometer (3 citations) Spectrophotometer UV-Vis Seri UV-1601 (2 citations)

187 protocols using uv 1601 spectrophotometer

Anthocyanin Content Analysis in Drupes

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The anthocyanins content on drupes was determined following the method of Raj and Ahmad [60 ], with modifications. Briefly, 2 g of fruit epicarp was macerated in 20 mL of 5% acidified methanol using a mortar and pestle. The extraction was repeated three times. The obtained extract was centrifuged at 6500 rpm for 10 min. The supernatant was kept in a dark environment overnight. Finally, the absorbance was measured at 520 nm using a Shimadzu UV-1601 spectrophotometer (Shimadzu, Kyoto, Japan).
The absorbance measurements were performed using a Shimadzu UV-1601 spectrophotometer (Shimadzu, Kyoto, Japan) at wavelength of 520 nm. The total anthocyanin was expressed as mg of cyanidin-3-glucoside equivalent per kg.

Cirillo A., Graziani G., De Luca L., Cepparulo M., Ritieni A., Romano R, & Di Vaio C. (2023). Minor Variety of Campania Olive Germplasm (“Racioppella”): Effects of Kaolin on Production and Bioactive Components of Drupes and Oil. Plants, 12(6), 1259.

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Free Radical Scavenging Activity of S. lateriflora

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The free radical scavenging activity of S. lateriflora extract was determined using the DPPH method [68 (link)]. The extract was tested at different concentrations ranging from 0.0625 to 2 mg/mL. An aliquot (0.5 mL) of a methanol solution containing different concentrations of the extract was added to 3 mL of freshly prepared methanol–DPPH solution (0.1 mM). After 20 min of initial mixing, the change in the optical density of the solution was measured at a wavelength of 517 nm using a model UV-1601 spectrophotometer (Shimadzu). Butylated hydroxytoluene (BHT) was used as a reference. The scavenging activity of the sample solution was measured as a decrease in its absorbance in comparison to the DPPH standard solution. The results averaged from three independent experiments were expressed both as mean radical scavenging activity (%) ± SD and mean 50% inhibitory concentration (IC₅₀) ± SD.

Kwiecień I., Miceli N., D’Arrigo M., Marino A, & Ekiert H. (2022). Antioxidant Potential and Enhancement of Bioactive Metabolite Production in In Vitro Cultures of Scutellaria lateriflora L. by Biotechnological Methods. Molecules, 27(3), 1140.

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Protein Production Using E. coli Strains

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E. coli strain BL21(DE3) was used for the isolation of Mt56(DE3) (see below)13 . Mt56(DE3), BL21(DE3), C41(DE3) and C43(DE3) were used for protein production experiments5 (link). All genes encoding membrane proteins used in this study as well as the gene encoding GFP were expressed from a pET28a + derived vector as described before33 (link). All membrane protein targets were produced as C-terminal GFP-His₈ fusions as described before33 (link). Cells were grown aerobically at 30 °C and 200 rpm in Lysogeny broth (LB) medium (Difco) supplemented with 50 μg/ml kanamycin. Growth was monitored by measuring the OD₆₀₀ with a UV-1601 spectrophotometer (Shimadzu). At an OD₆₀₀ of ~0.4 target gene expression was induced by adding 0.4 mM IPTG. For online GFP fluorescence measurements 200 μl of cultures were transferred after induction with IPTG at an OD₆₀₀ of ~0.4 to a 96 well plate and fluorescence was automatically detected every 5 minutes. The 96 well plate was shaken every 30 seconds36 (link).

Baumgarten T., Schlegel S., Wagner S., Löw M., Eriksson J., Bonde I., Herrgård M.J., Heipieper H.J., Nørholm M.H., Slotboom D.J, & de Gier J.W. (2017). Isolation and characterization of the E. coli membrane protein production strain Mutant56(DE3). Scientific Reports, 7, 45089.

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Synthesis and Characterization of Folate-Chitosan Conjugate

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FA-CS was prepared through an amino-acylation reaction (Fig. 1). Briefly, different concentrations of folate were dissolved into anhydrous DMSO with stirring. EDC (10 mol/ml) was added into the solution and stirred at room temperature for 1 h. Subsequently, 5 ml chitosan sodium acetate (pH 5.0: w/v 5%) was added to the solution, stirred at 30°C in the dark overnight. The resultant mixture was adjusted to pH 9.0 and then dialyzed against 0.1 M sodium phosphate buffer (pH 7.4) for 3 days, followed by dialysis against water for 3 days using a dialysis bag (molecular weight cut-off, 12 kDa). The mixture was frozen at −48°C for 12 h, and it was subsequently isolated using a FreeZone system (2.5L; Labconco, Kansas City, MO, USA); the synthesized product was obtained following 24 h.
The chemical structure of FA-CS was analyzed by infrared spectroscopy (IR; WGH-30; Gang Dong Technology Co., Ltd., Tianjing, China) and ¹H nuclear magnetic resonance (NMR; in D₂O, 500 MHz; Bruker Corporation, Billerica, MA, USA). The coupled number (the number of folate molecules to CS) of folate to chitosan was calculated based on the molar extinction coefficient value, which was determined by UV-1601 spectrophotometer (Shimadzu Corporation, Kyoto, Japan) at 363 nm.

Cheng L., Ma H., Shao M., Fan Q., Lv H., Peng J., Hao T., Li D., Zhao C, & Zong X. (2017). Synthesis of folate-chitosan nanoparticles loaded with ligustrazine to target folate receptor positive cancer cells. Molecular Medicine Reports, 16(2), 1101-1108.

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Oxidation Resistance of LDL Plasma

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The ex vivo resistance of LDL to Cu²⁺-induced oxidation was determined by monitoring the formation of conjugated dienes at 37 °C over 3 h with a Shimadzu UV1601 spectrophotometer at an absorbance of 234 nm according to Chen et al. [19 (link)]. The results are expressed as lag time (min). The FRAP value of whole plasma was determined by the spectrophotometric method of Benzie and Strain [20 (link)]. Total thiols (-SH moieties) in plasma were determined according to the spectrometric method of Hu [21 ].

Sawicki C.M., McKay D.L., McKeown N.M., Dallal G., Chen C.Y, & Blumberg J.B. (2016). Phytochemical Pharmacokinetics and Bioactivity of Oat and Barley Flour: A Randomized Crossover Trial. Nutrients, 8(12), 813.

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Quantifying MPO Activity in Liver Biopsies

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The MPO activity was measured in liver biopsies by the method of Kuebler et al [20 (link)]. Briefly, the tissue was homogenized with Tris-HCl buffer (0.1 M, pH 7.4) containing 0.1 M polymethylsulfonyl fluoride to block tissue proteases, and then centrifuged at 4°C for 20 min at 24.000 g. The MPO activities of the samples were measured at 450 nm (UV-1601 spectrophotometer; Shimadzu, Japan), and the data were referred to the protein content.

Strifler G., Tuboly E., Görbe A., Boros M., Pécz D, & Hartmann P. (2016). Targeting Mitochondrial Dysfunction with L-Alpha Glycerylphosphorylcholine. PLoS ONE, 11(11), e0166682.

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DPPH Scavenging Activity of I. tinctoria Extracts

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The free radical scavenging activity of hydroalcoholic extracts obtained from I. tinctoria shoot cultures was determined using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method [34 (link)]. The extracts were tested at different concentrations (0.0625–2 mg/mL). An aliquot (0.5 mL) of each sample solution was added to 3 mL of daily prepared methanol DPPH solution (0.1 mM). The optical density change at 517 nm was measured 20 min after the initial mixing using a model UV-1601 spectrophotometer (Shimadzu). The scavenging activity was measured as the decrease in absorbance of the sample’s vs. the DPPH control solution. Trolox (0.03–0.1 mg/mL) was used as a standard. The assays were carried out in triplicate, and the results are reported as mg of Trolox equivalents (TE)/g extract ± SD.

Miceli N., Kwiecień I., Nicosia N., Speranza J., Ragusa S., Cavò E., Davì F., Taviano M.F, & Ekiert H. (2023). Improvement in the Biosynthesis of Antioxidant-Active Metabolites in In Vitro Cultures of Isatis tinctoria (Brassicaceae) by Biotechnological Methods/Elicitation and Precursor Feeding. Antioxidants, 12(5), 1111.

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Production of Membrane Proteins in E. coli

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For protein production experiments the E. coli strains BL21(DE3), C41(DE3) and C43(DE3) were used [4 (link), 16 (link)]. All genes, but one, encoding the target proteins used in this study were expressed from a pET28a+ derived vector as described before [22 (link)]. The one exception is described in Additional file 1: Figure S4. All membrane protein targets were produced as C-terminal GFP-His₈ fusions as described before [27 (link)]. Cells were grown aerobically at 30 °C and 200 rpm, in Lysogeny broth (LB) medium (Difco) supplemented with 50 µg/ml kanamycin. At an A₆₀₀ of ~0.4 target gene expression was induced by adding 0.4 mM IPTG. Growth was monitored by measuring the A₆₀₀ with a UV-1601 spectrophotometer (Shimadzu). For online GFP fluorescence measurements 200 µl of the induced (or not induced) cultures were transferred at an A₆₀₀ of ~0.4 to a 96 well plate and fluorescence was automatically detected every 5 min. The 96 well plate was shaken every 30 s [13 (link)].

Zhang Z., Kuipers G., Niemiec Ł., Baumgarten T., Slotboom D.J., de Gier J.W, & Hjelm A. (2015). High-level production of membrane proteins in E. coli BL21(DE3) by omitting the inducer IPTG. Microbial Cell Factories, 14, 142.

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Assessing Synechocystis Cell Viability Post-Printing

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Cell viability of printed Synechocystis PCC 6803 was analysed by comparing the numbers of colonies before and after printing. The culture was grown in BG-11 high salt medium, and cells pelleted and resuspended to form bioink (a concentrated solution of cells in the medium) using the aforementioned method. Using the same printer and ink cartridge, as described earlier, the bioink was digitally printed onto a microscopic glass cover slip 22 × 22 mm Deckgläser (Menzel Gläser, Germany) and printed cells were immediately resuspended with pipetted distilled water and collected as a solution. The OD₇₃₀ of the suspension was measured to be 0.066 using a Shimadzu UV-1601 spectrophotometer. The suspension of cells before printing was sampled from the unused bioink and its OD₇₃₀ was adjusted to that of the suspension of printed cells. The cell suspension was serially diluted (x 0.1, × 0.01, × 0.001) (Fig. 1c) and a 3 µl droplet from each of the suspensions was spotted onto solid medium (BG-11, agar 1.5 % w/w) and incubated at 30 °C under continuous illumination of 40 µE m⁻² s⁻¹ from white fluorescent lamps. Chlorophyll was extracted and amount determined as described by Porra et al^{52 (link)}.

Sawa M., Fantuzzi A., Bombelli P., Howe C.J., Hellgardt K, & Nixon P.J. (2017). Electricity generation from digitally printed cyanobacteria. Nature Communications, 8, 1327.

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Quantifying Biocrust Photosynthetic Biomass

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Chlorophyll a areal concentration was used as a proxy for photosynthetic biomass. Biocrust cores (0.5 cm deep, 0.5 cm diameter) were collected and kept at 4°C in dark under dry conditions until analysis. Two cores were collected per container, yielding a total of 6 replicates per time point. Chlorophyll a was extracted in the dark at 4°C for 24 h following the Giraldo-Silva method described by Sorochkina et al. (53 (link)), after sample grinding by mortar and pestle in 90% acetone. The centrifuge-clarified samples (8,437 × g at 15°C for 10 min) were analyzed spectrophotometrically in a Shimadzu UV1601 spectrophotometer, according to the protocol of Garcia-Pichel and Castenholz (56 (link)), which corrects for scytonemin and carotenoid interference.

Bethany J., Giraldo-Silva A., Nelson C., Barger N.N, & Garcia-Pichel F. (2019). Optimizing the Production of Nursery-Based Biological Soil Crusts for Restoration of Arid Land Soils. Applied and Environmental Microbiology, 85(15), e00735-19.

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