3d system

Manufactured by Bio-Rad

The 3D system is a lab equipment product designed for scientific applications. It provides a platform for three-dimensional data collection and analysis. The core function of the 3D system is to enable users to capture, process, and visualize spatial information in a three-dimensional format.

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Lab products found in correlation

Bio plex pro human cytokine 27 plex assay kit, by Bio-Rad (1 mentions)

Spelling variants of this product

Bio-Plex 3D Suspension Array System (35 citations) FLEXMAP 3D system (9 citations) Bio-Plex 3D system (6 citations) 3D suspension array system (5 citations)

3 protocols using 3d system

Quantifying Cytokine Levels in Tissue

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Five-hundred microliters of 1x mammalian cell lysis buffer and 5 mm-stainless steel beads were added to frozen tissue samples in 2 mL microcentrifuge tubes. The samples were then homogenized using TissueLyser LT at 50 Hz for 10 min at 4 °C. Following homogenization, the samples were centrifuged at 10,000 × g for 10 min at 4 °C and the supernatants were transferred to new sample tubes. The samples were tested for six cytokines: IFN-γ, IL-1β, IL-6, IL-10, IL-17A, and TNF-α, using a Bio-Plex Pro Mouse Cytokine Th17 Panel A 6-Plex kit in accordance with the manufacturer’s protocol. The final 96-well plate was processed using the Bio-Plex 3D system and the concentration of each cytokine was determined using Bio-Plex Manager software.

Praveschotinunt P., Duraj-Thatte A.M., Gelfat I., Bahl F., Chou D.B, & Joshi N.S. (2019). Engineered E. coli Nissle 1917 for the delivery of matrix-tethered therapeutic domains to the gut. Nature Communications, 10, 5580.

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Quantifying FcR Binding Using Luminex Assay

Cited 1 time

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FcR binding was preformed using a multiplexed Luminex assay as described in Brown et al., 2012 (link) (Brown et al., 2012 (link)). Pertussis antigens were coupled to magplex microspheres as described above in coupling for phagocytosis assays. Recombinant FcRs with an AviTag were biotinylated on their avidin tag using a Bir500 kit according to manufacturer’s instructions. FcRs were then incubated with streptavidin-PE (ProZyme, Hayward, CA) for ten minutes, and any residual streptavidin was quenched with an excess of 20 μM biotin from the Bir500 kit for a further ten minutes. Plasma samples were diluted to three dilutions (1:50, 1:100 and 1:500) in luminex wash buffer (PBS-0.05% BSA-0.001% tween-20) and incubated with microspheres for two hours. Samples were washed in Luminex wash buffer and then incubated with PE-labeled FcRs for one hour. Samples were washed again and resuspended in xMAP sheath fluid. Samples were analyzed on a Bioplex 3D system. Data is calculated as area under the curve of the median fluorescent intensity of PE.

Jennewein M.F., Goldfarb I., Dolatshahi S., Cosgrove C., Noelette F.J., Krykbaeva M., Das J., Sarkar A., Gorman M.J., Fischinger S., Boudreau C.M., Brown J., Cooperrider J.H., Aneja J., Suscovich T.J., Graham B.S., Lauer G.M., Goetghebuer T., Marchant A., Lauffenburger D., Kim A.Y., Riley L.E, & Alter G. (2019). Fc glycan-mediated regulation of placental antibody transfer. Cell, 178(1), 202-215.e14.

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Quantifying Cytokine Levels in SFTS Serum

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We utilized the Bio-plex Pro Human cytokine 27-plex Assay kit (Bio-Rad, USA) to quantify the levels of cytokines in serum samples collected during the hospitalization of SFTS patients. This kit incorporates fluorescent microspheres that are conjugated with monoclonal antibodies that specifically target the cytokines of interest. To ensure accurate results, we strictly followed the instructions provided by the manufacturer, and all cytokines were tested using the same method. The following cytokines underwent testing: interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), Eotaxin, macrophage inflammatory protein 1β (MIP-1β), MIP-1α, monocyte chemotactic protein-1 (MCP-1), vascular endothelial growth factor (VEGF), basic fibroblast growth factor (basic FGF), granulocyte-macrophage colony-stimulating factor (GM-CSF), inducible protein-10 (IP-10), granulocyte colony-stimulating factor (G-CSF), interleukin (IL)-1β, IL-17, IL-15, IL-13, IL-12, IL-10, IL-9, IL-8, IL-7, IL-6, IL-5, IL-4, IL-2, IL-1 receptor antagonist (IL-1RA), regulated on activation and normally T-cell expressed (RANTES), and platelet-derived growth factor (PDGF-BB). Processed samples were subjected to testing using the Bio-Plex 3D system, which is a flow cytometry-based method.

Xu D.L., Zhang X.M., Tian X.Y., Wang X.J., Zhao L., Gao M.Y., Li L.F., Zhao J.Q., Cao W.C, & Ding S.J. (2024). Changes in Cytokine Levels in Patients with Severe Fever with Thrombocytopenia Syndrome Virus. Journal of Inflammation Research, 17, 211-222.

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