Human tumors were aseptically isolated and placed at room temperature in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% FBS plus 50 U/ml penicillin and 50 mg/ml streptomycin. Tumors were orthotopically implanted into the liver of two six-week-old male athymic nude mice (strain Hsd:Athymic Nude-Foxn1nu) purchased from Envigo. Briefly, mice were anesthetized with a continuous flow of 1% to 3% isoflurane and oxygen mixture (2 L/min). After performing a median laparotomy, the tumor fragment was anchored with a Prolene 7-0 suture into a small pocket created in the anterior hepatic lobe, and the abdominal incision will be closed with surgical staples. After implantation, mice were inspected twice a week, and at euthanasia, OPDX were harvested, cut into small fragments and serially transplanted into new animals for tumor perpetuation and/or experimental procedures. Animals were housed in a sterile environment, cages and water were autoclaved and bedding and food was γ-ray sterilized.
Athymic nude foxn1nu
Manufactured by Inotiv
Sourced in United States, United Kingdom
The Athymic Nude-Foxn1nu is a type of laboratory mouse model. It is characterized by the lack of a functional thymus gland, resulting in a deficiency of T cells and a compromised immune system.
Automatically generated - may contain errors
Lab products found in correlation
C b 17 icrhsd prkdc scid lyst bg j, by Inotiv (1 mentions)
C57bl 6 gt rosa 26sortm1 hbegf awai j, by Jackson ImmunoResearch (1 mentions)
Matrigel, by Corning (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Mda mb 231, by European Collection of Authenticated Cell Cultures (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Isoflurane cp, by CP-Pharma (1 mentions)
Matrigel, by BD (1 mentions)
Crizotinib, by MedChemExpress (1 mentions)
Rneasy micro, by Qiagen (1 mentions)
16 protocols using athymic nude foxn1nu
1
Orthotopic Liver Tumor Xenograft Model
2
Xenograft Mouse Models for Cancer
3
Orthotopic Breast Cancer Xenograft Model
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All animal experiments were carried out in accordance with the protocol approved by the University Health Network (UHN) Animal Care Committee. 6 to 8-week-old female mice (Athymic Nude-Foxn1nu, Envigo) were maintained at the UHN animal facility. The orthotropic tumor model was established by injecting 50 µl of MDA-MB-231/Luc cell suspension (1 × 106 cells per ml) into the inguinal mammary fat pad of athymic nude mice.
4
Xenograft Formation in Athymic Nude Mice
5
Nude Mice in Sterile Environment
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Six‐week‐old male Athymic Nude‐Foxn1nu (Envigo, Indianápolis, IN, USA) mice weighing 18 to 22 g were used in this study. Animals were housed in a sterile environment, in cages with autoclaved bedding, food, and water. The mice were maintained on a daily 12‐h light/12‐h dark cycle.
6
Subcutaneous and Metastatic Xenograft Assays
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Animal studies were conducted in compliance with guidelines for the care and use of laboratory animals and were approved by the Ethics and Animal Care Committee of Universidad de Cantabria. For proliferation assays, five million cells in 500 µl of PBS were subcutaneously injected into the flanks of 6-8-week-old female nude mice (Athymic Nude-Foxn1nu, Envigo, UK). 24 days after the injection, mice were euthanizing and tumor tissues were harvested for analyses. For metastasis assays, 2.5 million of cells in 500 µl of PBS with 0,1% BSA, were tail injected into 6-8-week-old female nude mice. Hairpin expression in the cells was induced with 1 µg/ml of Doxycycline 7 days before injection. After two months, mice were euthanizing and tumor tissues were harvested for analyses. In both cases, to keep the hairpin expression, the animals were treated from the day of injection with 2 mg/mL of Doxycycline in the drinking water supplemented with 1% sucrose refreshed every 2-3 days.
7
Culturing MDA-MB-231 Breast Cancer Cells
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Human breast cell line MDA-MB-231 was obtained from the European Collection of Authenticated Cell Cultures (ECACC) and cultured at 37°C in 5% CO2 humidity according to standard mammalian tissue culture protocols in Dulbecco’s Modified Eagle’s Medium + GlutaMax (Gibco, Waltham, MA) containing 10% fetal bovine serum (FBS) (Gibco, Waltham, MA) and 100 units/ml penicillin/100 μg/ml streptomycin (Gibco, Waltham, MA).
Athymic nude female mice (AthymicNude-Foxn1nu, ENVIGO, Indianapolis, IN) of 5 weeks of age were used to develop the breast cancer xenograft model.
Athymic nude female mice (AthymicNude-Foxn1nu, ENVIGO, Indianapolis, IN) of 5 weeks of age were used to develop the breast cancer xenograft model.
8
Inoculation of Athymic Nude Mice with Prostate Cancer Cells
9
Preclinical Pancreatic Cancer Xenograft Model
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De-identified human plasma samples were obtained from Nebraska Biobank. A human tissue array containing sections from pancreatic tumor and normal pancreas was obtained from US Biomax, Inc. De-identified normal and PC tissues were obtained from the UNMC tissue bank. All animal care procedures were carried out with approval from the Institutional Animal Care and Use Committee of the University of Nebraska Medical Center (Protocol Number: 18-034-04-FC). Six- to eight-week-old male and female, athymic nude mice (Strain: Hsd:Athymic Nude-Foxn1nu, Envigo, Indianapolis, IN, USA) and immunocompetent wild-type (mixed background) mice were used for the in vivo studies.
10
Athymic Nude Mice Xenograft Model
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All murine studies were performed following the approval of University of Colorado Anschutz Medical Campus Institutional Animal Care and Use Committee. Female Athymic Nude Mice (Hsd: Athymic Nude-Foxn1nu) aged 12–13 weeks were purchased from Envigo (Indianapolis, IN). MDA-MB-231 cells were collected during the logarithmic growth phase and injected subcutaneously at 5 × 106 cells on each flank under isoflurane anesthesia. The injection was 100ul per flank, consisting of a 1:1 ratio of DMEM media and cultrex (Cultrex PathClear BME, Type 3 from Bio-Techne). Treatment with either vehicle, 1.5 mg/kg doxorubicin IP QW, 80 mg/kg bocodepsin PO QD, or the combination was started when tumors reached an approximate average volume of 100mm3. Mice were monitored daily for toxicity, and weight and tumor volume were collected twice per week using a digital scale, calipers, and Studylog version 3.1.399.23 (San Francisco, CA). The following equation was used to calculate tumor volume: volume = (length x width2) x 0.52. Formalin-fixed paraffin-embedded tumor was collected at the end of study and stained for H&E, Ki67, and cleaved-caspase 3 by the UCCC Pathology Shared Resource.
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