Batch mode hydrodynamic size (diameter) measurements were performed on a Malvern Zetasizer Nano ZS90 (Malvern Instruments, Southborough, Massachusetts) with detection occurring at 90° to the light source. The emulsion was diluted 10 μL of emulsion into 90 μL of 1X TBS with 100 μM CaCl2 followed by equilibration (typically 2 minutes) in the DLS at 25°C before a minimum of three measurements per sample were made. After dilution, the system was checked for multi-particle scattering by testing 10 μL of emulsion diluted into 190 μL of 1x TBS with 100 μM CaCl2 to ensure that it gave the same particle diameter. Then 5 μL of 3.227 g/L LecA was added to the cuvette and mixed by pipetting the solution 10 times. A measurement of hydrodynamic size (determined by cumulants average or Z- average) via three measurements was then taken every 10 minutes for 2 hours. Only results with a polydispersity index (PDI) less than 0.3 were used for fitting to minimize errors in calculating particle diameter by method of cumulants. Zeta potential was also measured on the Malvern Zetasizer Nano ZS90 via three measurements. This measurement was conducted twice for each emulsion system.
Malvern zetasizer nano zs90
Manufactured by Malvern Panalytical
Sourced in United Kingdom, United States
The Malvern Zetasizer Nano ZS90 is a dynamic light scattering (DLS) instrument used for the measurement of particle size, zeta potential, and molecular weight. It determines the size of particles and molecules in the range of 0.3 nanometers to 10 micrometers. The instrument uses a laser light source and detects the Brownian motion of particles or molecules in a sample to calculate their size distribution.
Automatically generated - may contain errors
Lab products found in correlation
Escalab 250xi, by Thermo Fisher Scientific (3 mentions)
Jem 2100f, by JEOL (3 mentions)
Cary 100, by Agilent Technologies (2 mentions)
Fluoromax 4 spectrofluorometer, by Horiba (2 mentions)
Zetasizer nano zs90, by Malvern Panalytical (2 mentions)
Malvern nano zs90, by Malvern Panalytical (2 mentions)
D8 advance, by Bruker (2 mentions)
H 600, by Hitachi (2 mentions)
2010 lab6, by JEOL (2 mentions)
Lambda 1050, by PerkinElmer (2 mentions)
Geminisem 300, by Zeiss (2 mentions)
Jem 2100, by JEOL (2 mentions)
Jem 1400, by JEOL (1 mentions)
Lambda 25 spectrometer, by PerkinElmer (1 mentions)
Folded capillary cell dts1060, by Malvern Panalytical (1 mentions)
H 7600, by Hitachi (1 mentions)
Ftir spectroscopy, by Thermo Fisher Scientific (1 mentions)
Tecnai g2 20, by Thermo Fisher Scientific (1 mentions)
Jen 1230, by JEOL (1 mentions)
Jem 1230, by JEOL (1 mentions)
184 protocols using malvern zetasizer nano zs90
1
Hydrodynamic Size Determination of Emulsions
2
Synthesis and Characterization of Bovine Serum Albumin-based Bioimaging Nanocomposites
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The BIL was synthesized by co-assembly of BSA, IR783 and LA according the literature.31–33 (link) Typically, BSA (80 mg), GSH (75 mg), IR783 (20 mg) and LA (20 mg) were dissolved in 5 mL of PBS at pH = 7.4 under stirring. Then the mixture was sonicated for 5 min and stirred for another 30 min. Subsequently, ethanol (2 mL) was dropped into the above solution under stirring. After 0.5 h, the reaction mixture was dialyzed (MWCO 100 000) against deionized water to remove the extra reactants. The BIL composites were collected by lyophilization of the supernatant after centrifugation at 20 000g for 10 min (yield: 108 mg). The BSA–IR783 (BI) and BSA–LA (BL) composites were synthesized by the same method without addition of LA or IR783. The morphology was observed by TEM (JEOL JEM-1400, Japan). UV-vis absorption spectra were measured on a LAMBDA 25 spectrometer (PerkinElmer) and the loading amount of IR783 in BIL was determined by UV-Vis absorption spectra (18%, 180 μg mg−1). The loading amount of LA was analyzed using a human l -arginine ELISA kit (12%, 120 μg mg−1). Zeta potentials and hydrodynamic diameters were measured using a Malvern Zetasizer Nano ZS90 (Malvern Instruments Ltd., UK).
3
Chitosan-CNC Particle Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The electrophoretic mobility and z-average hydrodynamic diameter of chitosan–CNC PMC particles were measured by laser Doppler electrophoresis in a Malvern Zetasizer Nano ZS90 (Malvern Instruments, Inc., Westborough, MA, USA). Samples were prepared by mixing of a CNC suspension (0.02% (w/v)) and a chitosan solution (0.001% (w/v)), each with an ionic strength of 1 mM and a pH of 2.6, under strong agitation with a magnetic stir bar. The volumes of the two liquids were chosen to give sulfate/amino group molar ratio (S/N ratio) of 0.3. The obtained PMC particle suspensions were used without further dilution or filtration. Each measurement was performed at 25 °C in a Malvern DTS1060 folded capillary cell and was repeated two times.
4
Particle Size Determination of NLC
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The mean particle size of NLC formulations was estimated using Malvern® Zetasizer Nano ZS90 (Malvern® Instruments Limited, Worcestershire, UK). After diluting with distilled water (1:200), triplicate measurements were made using a 90° scattering angle ( Elmowafy et al., 2017 (link)
) .
5
Particle Size and Zeta Potential Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The particle size and zeta potential of blank NPs and Cur-NPs were investigated using a dynamic light scattering (DLS) laser particle size analyzer (Malvern Zetasizer Nano-ZS 90, Malvern Instruments Ltd, UK) equipped with a He-Ne laser (633 nm) at 25°C. Data were evaluated based on intensity distribution. Each sample was measured in deionized water, the test was repeated three times, and all data were expressed as the mean±standard deviation.
6
Characterization of Self-Assembled AlMA Aggregates
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To induce self-assembled
aggregates, AlMA (0, 0.05, 0.6, and 2% DM; 2 mg/mL) was dissolved
in PBS and allowed to stir in the dark at room temperature. The hydrodynamic
diameter and polydispersity index (PdI) of the resulting self-assembled
AlMA aggregates were measured by dynamic light scattering (DLS) using
a nanoparticle analyzer (Malvern Zetasizer Nano ZS90, Malvern Instruments,
Malvern, UK).
Transmission electron microscopy (TEM) analysis
was conducted to study the aggregation of 20% AlMA. AlMA macromolecule
solution was applied to a charged carbon–formvar-coated grid
and stained (1% uranyl acetate, 1 min). The samples were then examined
with the TEM (Hitachi H7600, Tokyo, Japan) at 80 kV. High-DM AlMA
solution specimens with a concentration range of 0.1 ng/mL–10
mg/mL were subject to TEM measurements.
aggregates, AlMA (0, 0.05, 0.6, and 2% DM; 2 mg/mL) was dissolved
in PBS and allowed to stir in the dark at room temperature. The hydrodynamic
diameter and polydispersity index (PdI) of the resulting self-assembled
AlMA aggregates were measured by dynamic light scattering (DLS) using
a nanoparticle analyzer (Malvern Zetasizer Nano ZS90, Malvern Instruments,
Malvern, UK).
Transmission electron microscopy (TEM) analysis
was conducted to study the aggregation of 20% AlMA. AlMA macromolecule
solution was applied to a charged carbon–formvar-coated grid
and stained (1% uranyl acetate, 1 min). The samples were then examined
with the TEM (Hitachi H7600, Tokyo, Japan) at 80 kV. High-DM AlMA
solution specimens with a concentration range of 0.1 ng/mL–10
mg/mL were subject to TEM measurements.
7
Particle Size and Zeta Potential Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Particle size and zeta potential were carried out using a Malvern Zetasizer Nano ZS90 (Malvern Instruments Ltd., Worcestershire, UK). The PGWP and PGWP-SIF solutions were diluted to a protein concentration of 0.1% with deionized water [15 (link)]. The refractive indexes for protein and water were 1.450 and 1.333, respectively. All measurements were performed in triplicate.
8
Nanoparticle Purification and Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The nanoparticles thus formed by self-assembly were subjected to ultracentrifugation using 30 KDa filtered centrifugal tubes (MilliporeSigma, Burlington, MA, USA) at 600 g for 15 min at 25 °C to remove non-encapsulated free drug and water. These nanoparticles were measured for their size, zeta potential and PDI using Malvern Zetasizer Nano ZS90 (Malvern Panalytical Inc., Westborough, MA, USA) based on the principle of Dynamic Light Scattering (DLS).
9
Characterization of PVP-coated AgNPs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Polyvinylpyrrolidone (PVP)-coated AgNPs were purchased from Shanghai Huzheng Nano Technology Co., Ltd. (Shanghai, China). The AgNPs morphology was characterized by transmission electron microscope (TEM, JEOL, Japan). The dynamic light scattering of AgNPs in cell culture medium was measured using Malvern Zetasizer Nano-ZS90 instrument (Malvern Instrument Ltd., UK). The maximum absorbance peak of AgNPs was analyzed by ultraviolet visible (UV–vis) spectroscopy.
10
Zeta Potential and Nanoemulsion Permeation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Example 16
The purpose of this example was to measure the zeta potential of different nanoemulsions and to correlate the zeta potential with improved epidermal and dermal permeation of the component quaternary ammonium compound.
The mean particle size (Z-AVE), polydispersity index (PdI) and zeta potential were determined for samples by dynamic light scattering using photon correlation spectroscopy in a Malvern Zetasizer Nano ZS90 (Malvern Instruments, Worcestershire, UK). For particle size, the test sample of nanoemulsion diluted was to 1% final nanoemulsion concentration. For zeta potential, the test sample of nanoemulsion diluted was to 0.1% final nanoemulsion concentration. All measurements were carried out at 25° C. after appropriate dilution with double distilled 0.2 μm filtered water.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!