The fluorescent tracers TxRed 3kDa and Cs Blue 10kD were purchased from Invitrogen (Waltham, MA). 14C-Aminoisobutyric acid was obtained from American Radiolabeled Chemicals (ARC) (St. Louis, MO). Fetal Bovine Serum, phosphate-buffered saline (PBS) and Dulbecco’s modified eagle medium were obtained from Gibco™-Thermo Fisher Scientific (Waltham, MA). Paclitaxel was purchased from Selleck Chemicals (Houston, TX). Liposomal Doxil was kindly donated by West Virginia University Hospital Department of Pharmacy. IVISbrite D-Luciferin Potassium Salt Bioluminescent Substrate was obtained from PerkinElmer (Waltham, MA). Cremophore EL was purchased from Sigma-Aldrich (St. Louis, MO). All reagents and chemicals used were analytical grade.
Ivisbrite d luciferin potassium salt bioluminescent substrate
Manufactured by PerkinElmer
Sourced in United States
IVISbrite D-Luciferin Potassium Salt Bioluminescent Substrate is a lab equipment product manufactured by PerkinElmer. It is a bioluminescent substrate used for in vivo and in vitro applications involving luciferase reporter systems.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Paclitaxel, by Selleck Chemicals (1 mentions)
Cremophore el, by Merck Group (1 mentions)
Dulbecco s modified eagle medium, by Thermo Fisher Scientific (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
Lago x optical imaging system, by Spectral Instruments Imaging (1 mentions)
2 protocols using ivisbrite d luciferin potassium salt bioluminescent substrate
1
Fluorescent Tracer and Radiolabeled Compound Protocols
2
Peritoneal Tumor Burden Quantification
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Because caliper measurement is not feasible for measuring peritoneal tumor burden, we used bioluminescent imaging as a surrogate for tumor burden, as previously described.49 50 (link) All animals were imaged with bioluminescence for luciferase activity in the peritoneum to identify peritoneal tumor implants and growth after IP SNU-16-ffluc, and the tumor burden was quantified once a week after treatment. D-luciferin solution was prepared by dissolving 1 g of IVISbrite D-Luciferin Potassium Salt Bioluminescent Substrate (PerkinElmer, catalog#122799-5, Waltham, Massachusetts, USA) in 35 mL of PBS at 28.5 mg/mL concentration. IP delivery (200 µL/mouse) was performed in all groups, and the mice were imaged using Lago X optical imaging system (Spectral Instruments Imaging, Tucson, Arizona, USA). Bioluminescence imaging was analyzed using Aura V.64 software and presented as photons/second for regions of interest.41 (link)
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