Agilent 7700x icp ms

Manufactured by Agilent Technologies

Sourced in Japan, United States

The Agilent 7700x ICP-MS is a high-performance inductively coupled plasma mass spectrometer (ICP-MS) designed for the analysis of trace elements in a variety of sample types. It provides accurate and sensitive detection of elements at parts-per-trillion (ppt) levels.

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41 protocols using agilent 7700x icp ms

HPLC-ICP-MS for Arsenic Speciation

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An Agilent 1260 HPLC system comprising a quaternary pump, autosampler and vacuum degasser was used for As species separation, coupled to an Agilent 7700x ICP-MS (Agilent, Santa Clara, CA, USA) for detection. The column exit was simply connected to the nebulizer of the ICP-MS by PFA tubing. The reverse phase column Agilent ZORBAX SB-Aq (4.6 mm id × 250 mm, 5 μm) was maintained at room temperature throughout the analysis. The mobile phase was 20 mM citric acid and 5 mM sodium hexanesulfonate, adjusted to pH 4.3 with 2 M sodium hydroxide. The injection volume was 10 µL; a flow rate of 1.0 mL·min⁻¹ was adopted, with a run time of 3.7 min.
An Agilent 7700x ICP-MS with an Octopole Reaction System (ORS³, Santa Clara, CA, USA) collision/reaction cell (CRC) was used for As species detection. The 7700 ICP-MS was fitted with a standard Micromist nebulizer and a Scott spray chamber (temperature controlled at 2 °C). Typical operating parameters for ICP-MS were: RF power, 1550 W; carrier gas flow rate, 0.8 L·min⁻¹; makeup gas flow rate, 0.4 L·min⁻¹; sampling depth; 8.0 mm.
Helium cell gas was used to rule out potential polyatomic ion interference (⁴⁰Ar³⁵Cl⁺ or ⁴⁰Ca³⁵Cl⁺) on ⁷⁵As⁺. The helium cell gas flow rate was 4.0 mL·min⁻¹. ¹⁰³Rh was used as post-column online internal standard to correct long-term signal drift caused by the plasma matrix.

Shi Q., Ju M., Zhu X., Gan H., Gu R., Wu Z., Meng Z, & Dou G. (2019). Pharmacokinetic Properties of Arsenic Species after Intravenous and Intragastrical Administration of Arsenic Trioxide Solution in Cynomolgus Macaques Using HPLC-ICP-MS. Molecules, 24(2), 241.

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Quantitative Elemental Analysis of Whole Blood

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200 µl of whole blood was transferred into a 15-ml polyethylene tube and diluted 1:25 with a diluent solution consisting of 0.1% (v/v) Triton X-100, 0.1% (v/v) HNO₃, 2 mg/l AU, and internal standards (20 µg/l). The mixture was sonicated for 10 min before inductively coupled plasma-mass spectrometry (ICP-MS) analysis. Multi-element determination was performed on an Agilent 7700x ICP-MS (Agilent Technologies, Tokyo, Japan) equipped with an octupole reaction system (ORS) collision/reaction cell technology to minimize spectral interferences. The continuous sample introduction system consisted of an autosampler, a quartz torch with a 2.5-mm diameter injector with a Shield Torch system, and a Scott double-pass spray chamber and nickel cones (Agilent Technologies). A glass concentric MicroMist nebuliser (Agilent Technologies) was used for the analysis of diluted samples.

Jie Z., Chen C., Hao L., Li F., Song L., Zhang X., Zhu J., Tian L., Tong X., Cai K., Zhang Z., Ju Y., Yu X., Li Y., Zhou H., Lu H., Qiu X., Li Q., Liao Y., Zhou D., Lian H., Zuo Y., Chen X., Rao W., Ren Y., Wang Y., Zi J., Wang R., Liu N., Wu J., Zhang W., Liu X., Zong Y., Liu W., Xiao L., Hou Y., Xu X., Yang H., Wang J., Kristiansen K, & Jia H. (2021). Life History Recorded in the Vagino-cervical Microbiome Along with Multi-omes. Genomics, Proteomics & Bioinformatics, 20(2), 304-321.

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Urinary Cadmium Measurement Protocol

Cited 2 times

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Spot urine samples from baseline were collected in polypropylene tubes,
frozen within 1 to 2 hours of collection, shipped buried in dry ice and stored
in freezers at −70°C in the Penn Medical Laboratory, MedStar
Research Institute, Washington, DC. Strict controls on the sampling, transport
and storage of urine were conducted to ensure study quality (Strong Heart Study, 1991 ). The analyses of cadmium
and other metals were performed by Inductively Coupled Plasma Mass Spectrometry
ICP-MS (Agilent 7700x ICP-MS, Agilent Technologies, Waldbronn, Germany) and
urine samples have already been used to measure creatinine and albumin (Tellez-Plaza et al, 2013b (link); Scheer et al., 2012 (link)). The inter-assay and the
intra-assay coefficients of variation for urinary cadmium concentrations were
8.7% and 4.5%, respectively. Standard reference materials
(National Institute of Standards and Technology, NIST 1640a and 1643e) were used
to test the accuracy of the analyses. The limit of detection for urine cadmium
was 0.015 μg/L (and the corresponding limit of quantification is 0.050
μg/L), but our limit of detection is estimated conservatively so we kept
all values provided by the method above the limit of detection. A total of 87
(5.05%) samples were below the limit of detection and were replaced by
the limit of detection divided by the square root of two.

Olmedo P., Grau-Perez M., Fretts A., Tellez-Plaza M., Gil F., Yeh F., Umans J.G., Francesconi K.A., Goessler W., Franceschini N., Lee E.T., Best L.G., Cole S.A., Howard B.V, & Navas-Acien A. (2016). Dietary determinants of cadmium exposure in the Strong Heart Family Study. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 100, 239-246.

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Vacuole Isolation and Cytosol Analysis

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The cytosol was isolated from fermenting cells (pH 5) and filtered through a 10-kDa cutoff membrane as described (55 (link)). Filtered FTS was injected into a treated size-exclusion (SEC) Superdex Peptide 10/300 Gl column (GE Life Sciences) connected to an Agilent 1260 Bioinert quaternary pump (G5611A) and Agilent 7700x ICP-MS. The mobile phase was 20 mM ammonium acetate pH 6.5 flowing at 0.6 ml/min. Vacuoles were isolated as described (12 (link)).

Kim J.E., Vali S.W., Nguyen T.Q., Dancis A, & Lindahl P.A. (2020). Mössbauer and LC-ICP-MS investigation of iron trafficking between vacuoles and mitochondria in vma2ΔSaccharomyces cerevisiae. The Journal of Biological Chemistry, 296, 100141.

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Quantifying Soluble Metal Impurities in MWCNTs

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To evaluate the soluble metal impurities of MWCNTs, fibers were dispersed and incubated for 24 h in phosphate-buffered saline (PBS; Sigma-Aldrich, St. Louis, MO, USA) or artificial lysosomal fluid (ALF), which was used to mimic interstitial fluid or lysosomal fluid, respectively. To prepare ALF, 55 mM of NaCl, 150 mM of NaOH, 108 mM of citric acid, 0.87 mM of CaCl₂, 0.67 mM of Na₂HPO₄·7H₂O, 0.27 mM of Na₂SO₄, 0.52 mM of MgCl₂·6H₂O, 0.64 mM of glycerin, 0.26 mM of sodium citrate dehydrate, 0.39 mM of sodium tartrate dihydrate, 0.76 mM of sodium lactate, 0.78 mM of sodium pyruvate, and 1 mL of formaldehyde were mixed, and the pH was adjusted to 5.5 [23 (link)] (Stopford et al., 2003). MWCNTs were dispersed in PBS or ALF at 100 μg/mL, sonicated in a bath sonicator for 1 h, and incubated for 24 h at room temperature. Ultracentrifugation at 50,000 rpm for 3 h was performed, using an Optima AUC (Beckman Coulter; Indianapolis, IN, USA) to collect the MWCNT-free supernatants, and the concentration of metals was analyzed by using an Agilent 7700x ICP-MS (Agilent, Santa Clara, CA, USA). SEM was used to confirm the absence of MWCNT contamination in the supernatant. To prepare for SEM observations, 100 μL of undiluted supernatant was placed on the SEM filter (Merck Millipore Ltd., Burlington, MA, USA) and dried overnight at room temperature.

Lee D.K., Jeon S., Jeong J., Yu I.J., Song K.S., Kang A., Yun W.S., Kim J.S, & Cho W.S. (2020). Potential Role of Soluble Metal Impurities in the Acute Lung Inflammogenicity of Multi-Walled Carbon Nanotubes. Nanomaterials, 10(2), 379.

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Measurement of Lead in Erythrocytes

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Venous blood samples were collected at baseline after overnight fast. Plasma and erythrocytes were separated, and lead was measured in erythrocytes using inductively coupled plasma mass spectrometry with an octopole reaction system in helium mode (Agilent 7700x ICP-MS; Agilent Technologies) at the Department of Clinical Chemistry, Sahlgrenska University Hospital, Gothenburg, Sweden (Gambelunghe et al. 2016 (link); Harari et al. 2018 (link)). Lead in whole blood was then calculated by multiplying the metal concentrations in erythrocytes by the hematocrit. None of the samples were below the limit of detection, which was

0.16 μ g / L

. External quality control samples with low lead levels (Seronorm Trace Elements Whole Blood L-1, Lot no. 1103128; Sero AS) were included in all analytical rounds and showed satisfactory results:

mean \pm standard   deviation

(SD)

10.3 \pm 0.5 μ g / L

vs. recommended limits of

6 - 14 μ g / L

. The imprecision (coefficient of variation) was 5.1%, calculated from duplicate samples.

Harari F., Barregard L., Östling G., Sallsten G., Hedblad B., Forsgard N., Borné Y., Fagerberg B, & Engström G. (2019). Blood Lead Levels and Risk of Atherosclerosis in the Carotid Artery: Results from a Swedish Cohort. Environmental Health Perspectives, 127(12), 127002.

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Quantitative Analysis of Toxic Metals in Whole Blood

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Whole blood concentrations of manganese (Mn), cobalt (Co), nickel (Ni), arsenic (As), cadmium (Cd) and lead (Pb) were measured at the Lumigen Instrument Center at Wayne State University. Isotope dilution mass spectrometry [34 (link)] was implemented to determine absolute quantitation of toxic metals and metalloids in the blood samples using the Agilent 7700x ICP-MS. Whole blood was diluted 1:1 with 0.1% TritonX-100 then further diluted with 8 volumes of 2% nitric acid for a 10-fold dilution. Proteins that precipitate upon addition of the nitric acid were removed by centrifugation. Final dilution of supernatants was made with 2% nitric acid to achieve a final 50-fold dilution. A six-point standard curve was generated using a mixed standard containing all the metals of interest diluted in 2% nitric acid. The standard curve was run before and after the analysis of the samples. Each sample was analyzed three times and the results averaged. Replicate %CV values for the six metals were: Mn, 3.5; Co, 29.5; Ni, 8.7; As, 7.4; Cd, 9.9; Pb, 30.8.

Arnetz B.B., Sudan S., Arnetz J.E., Yamin J.B., Lumley M.A., Beck J.S., Stemmer P.M., Burghardt P., Counts S.E, & Jamil H. (2020). Dysfunctional neuroplasticity in newly arrived Middle Eastern refugees in the U.S.: Association with environmental exposures and mental health symptoms. PLoS ONE, 15(3), e0230030.

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Urine Cd Measurement Protocol

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Morning spot urine samples were collected in polypropylene tubes, frozen within 1 to 2 h of collection, shipped buried in dry ice, and stored at

- 70 ° C

in the Penn Medical Laboratory, MedStar Research Institute, Washington, DC, USA. In 2009, urine samples were shipped to the Trace Metals Laboratory at Graz University, Austria, to measure Cd and other metals using inductively coupled plasma–mass spectrometry (ICP-MS) (Agilent 7700x ICP–MS; Agilent Technologies). The limit of detection (LOD) for urine Cd was

0.015 μ g / L

. In one participant below the LOD, the Cd concentration was imputed as the LOD divided by the square root of two. Urine Cd concentrations were corrected for molybdenum oxide interference. Other laboratory details and extensive quality control/quality assurance have been published (Scheer et al. 2012 (link)). To account for urine dilution, urine Cd concentrations were expressed in micrograms per gram of urine creatinine. Urine creatinine was measured at the Laboratory of the National Institute of Diabetes and Digestive and Kidney Disease, Epidemiology and Clinical Research Branch (Phoenix, Arizona, USA) by an alkaline picrate rate method.

Domingo-Relloso A., Riffo-Campos A.L., Haack K., Rentero-Garrido P., Ladd-Acosta C., Fallin D.M., Tang W.Y., Herreros-Martinez M., Gonzalez J.R., Bozack A.K., Cole S.A., Navas-Acien A, & Tellez-Plaza M. (2020). Cadmium, Smoking, and Human Blood DNA Methylation Profiles in Adults from the Strong Heart Study. Environmental Health Perspectives, 128(6), 067005.

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Platinum complex 5a uptake in HT29 cells

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HT29 cells were seeded in 10 cm dishes at densities of 6 × 10⁶ cells/dish and kept at 37°C in a humidified 5% CO₂ incubator. After 2 h incubation with complete medium containing 200 μM of platinum complex 5a, the medium was removed, and the cells were washed with ice-cold PBS, trypsinized, and resuspended in 1 mL of PBS. The cell suspensions were centrifuged at 2500 rpm for 10 min and remove the PBS. Vortex vigorously to resuspend cells. Nuclei lysis solution was added to lyse the cells. DNA was extracted with TIANamp Genomic DNA kit (TIANGEN). The extracted DNA was dissolved in concentrated nitric acid (0.05 mL) and diluted with 4.95 mL of deionized water (final concentration 1% v/v) for determining the platinum content. The platinum content taken up by the cells was determined by Agilent 7700X ICP-MS (Agilent Technologies, Tokyo, Japan). Calibrations were done using standard solutions containing 6.25, 12.5, 25, 50 and 100 ppb platinum.

Liu R., Fu Z., Zhao M., Gao X., Li H., Mi Q., Liu P., Yang J., Yao Z, & Gao Q. (2017). GLUT1-mediated selective tumor targeting with fluorine containing platinum(II) glycoconjugates. Oncotarget, 8(24), 39476-39496.

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Trace and Mineral Analysis of Rat Serum

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Samples were diluted at a 1/10 ratio after acidic digestion via microwave digestion and used for the ICP-MS analysis. The trace and mineral element levels of the rat serum samples were evaluated using Agilent 7700x ICP-MS (Agilent Technologies Inc., Tokyo, Japan). External calibration solutions were prepared using the Spex Certiprep Multi-element calibration standard (2A). Data analysis was performed via MassHunter software (Aydemir et al., 2020c (link)).

Aydemir D., Malik A.N., Kulac I., Basak A.N., Lazoglu I, & Ulusu N.N. (2022). Impact of the Amyotrophic Lateral Sclerosis Disease on the Biomechanical Properties and Oxidative Stress Metabolism of the Lung Tissue Correlated With the Human Mutant SOD1G93A Protein Accumulation. Frontiers in Bioengineering and Biotechnology, 10, 810243.

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