Nci h211

Manufactured by American Type Culture Collection

Sourced in United States

The NCI-H211 is a cell line derived from a human lung carcinoma. It is maintained in culture and available for research purposes.

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6 protocols using nci h211

SCLC Cell Lines and PDX Cultivation

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NCI‐H211, NCI‐H524, DMS‐114, NCI‐H841, NCI‐H1048, NCI‐H1341, NCI‐H446, NCI‐H146, NCI‐H889, and U2OS DRGFP (Nakanishi et al, 2011 (link)) cell lines were purchased from ATCC. NCI‐H211, NCI‐H889, NCI‐H1048, NCI‐H1341, and U2OS DRGFP cell lines are female and the rest are male, additional information for cell lines can be observed in Table 1. Cell lines were authenticated using short tandem repeat analysis, and were monthly tested for mycoplasma contamination. PDX‐03 and PDX‐06 were derived from a male and a female SCLC patient, respectively. Cell medium was RPMI‐1640 supplemented with 10% FBS for all lines to maintain consistency. DT40 (chicken cell lines) were grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with FBS 10% and chicken serum 5%. Cells were grown at 37°C and 5% CO₂.

Schultz C.W., Zhang Y., Elmeskini R., Zimmermann A., Fu H., Murai Y., Wangsa D., Kumar S., Takahashi N., Atkinson D., Saha L.K., Lee C., Elenbaas B., Desai P., Sebastian R., Sharma A.K., Abel M., Schroeder B., Krishnamurthy M., Kumar R., Roper N., Aladjem M., Zenke F.T., Ohler Z.W., Pommier Y, & Thomas A. (2023). ATR inhibition augments the efficacy of lurbinectedin in small‐cell lung cancer. EMBO Molecular Medicine, 15(8), e17313.

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Cell Culture Protocols for HEK293T, SCLC, and Drosophila S2 Cells

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HEK293T cells were obtained from American Type Culture Collection (ATCC) and then maintained with Dulbecco’s modified Eagle’s medium (Gibco, Gaithersburg, MD) containing 10% fetal bovine serum (FBS; Sigma-Aldrich). The SCLC cell lines NCI-H526, NCI-H211, NCI0H510, and NCI-H1963 were obtained from ATCC and were maintained with ATCC-formulated RPMI 1640 medium containing 10% FBS (Sigma-Aldrich). The Drosophila S2 cells were maintained in HyClone SFX-Insect Cell Culture Media containing 10% FBS (Sigma-Aldrich).

Szczepanski A.P., Tsuboyama N., Watanabe J., Hashizume R., Zhao Z, & Wang L. (2022). POU2AF2/C11orf53 functions as a coactivator of POU2F3 by maintaining chromatin accessibility and enhancer activity. Science Advances, 8(40), eabq2403.

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Cell Line Maintenance Protocol

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HEK293T cells were purchased from ATCC (CRL-3216), and maintained with DMEM (Fisher Scientific, #15013CV) containing 10% FBS (MT35010CV, Sigma). The SCLC cell lines NCI-H526 (CRL-5811) and NCI-H211 (CRL-5824) were obtained from ATCC. The SCLC cell lineCORL311 was purchased from sigma (96020721). All the SCLC cell lines were maintained with ATCC-formulated RPMI-1640 medium (A1049101, Fisher Scientific) containing 10% FBS (MT35010CV, Sigma).

Szczepanski A., Tsuboyama N., Lyu H., Wang P., Beytullahoglu O., Zhang T., Singer B.D., Yue F., Zhao Z, & Wang L. (2024). A SWI/SNF-dependent transcriptional regulation mediated by POU2AF2/C11orf53 at enhancer. Nature Communications, 15, 2067.

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SCLC and Neuroblastoma Cell Line Culture

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The SCLC cell lines NCI-H82, NCI-H446,
NCI-H211, NCI-H524, NCI-H526, NCI-H146, NCI-H841, and NCI-H209, and
the neuroblastoma cell line SK-N-BE(2) were obtained from American
Type Culture Collection (ATCC, USA). All SCLC cell lines were maintained
in RPMI1640 medium (ThermoFisher Scientific, USA) supplemented with
10% fetal bovine serum (FBS, Hyclone, USA) and antibiotics. SK-N-BE(2)
was maintained in Minimum Essential Medium (MEM, ThermoFisher Scientific,
USA) supplemented with 10% FBS (HyClone, USA) and antibiotics.

Chi Y.H., Yeh T.K., Ke Y.Y., Lin W.H., Tsai C.H., Wang W.P., Chen Y.T., Su Y.C., Wang P.C., Chen Y.F., Wu Z.W., Yeh J.Y., Hung M.C., Wu M.H., Wang J.Y., Chen C.P., Song J.S., Shih C., Chen C.T, & Chang C.P. (2021). Discovery and Synthesis of a Pyrimidine-Based Aurora Kinase Inhibitor to Reduce Levels of MYC Oncoproteins. Journal of Medicinal Chemistry, 64(11), 7312-7330.

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SCLC Cell Line Maintenance Protocol

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All SCLC cell lines, including NCI-H82, NCI-H446, NCI-H69, NCI-H211, NCI-H524, NCI-H526, NCI-H146, NCI-H187, NCI-H345, NCI-H841, NCI-H1930, NCI-H2171, and NCI-H2081, were obtained from American Type Culture Collection (ATCC). NCI-H345, NCI-H841, NCI-H2171, and NCI-H2081 cell lines were maintained in HITES medium [Dulbecco’s medium: Ham’s F12, 50:50 mix (Gibco), insulin 0.005 mg/ml, transferrin 0.01 mg/ml, sodium selenite 30 nM, hydrocortisone 10 nM, and beta-estradiol 10 nM] containing 5% fetal bovine serum (FBS, Hyclone) and penicillin–streptomycin antibiotics (Gibco). The remainder of the cell lines were cultured in RPMI1640 medium (Gibco) supplemented with 10% FBS and antibiotics. Cells were cultured for up to 8 weeks and used at passages of less than 16 from the initial source vial.

Hung M.C., Wang W.P, & Chi Y.H. (2022). AKT phosphorylation as a predictive biomarker for PI3K/mTOR dual inhibition-induced proteolytic cleavage of mTOR companion proteins in small cell lung cancer. Cell & Bioscience, 12, 122.

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Culturing SCLC Cell Lines for Research

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The human SCLC cell lines NCI-H841, NCI-H1048, NCI-889, NCI-2171, NCI-146, NCI-H1963, HCC33 and NCI-H211 were obtained from the American Type Culture Collection (Manassas, VA, USA). Cells were grown in RPMI 1640 with 10% FetalClone (Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C with 5% CO₂ in a humidified incubator, except for NCI-H2171 and NCI-H889 cells, which were grown in a HITES medium (DMEM/F12 supplemented with 1% Glutamax, 100 U/mL penicillin, 100 µg/mL streptomycin, 4 µg/mL hydrocortisone (Sigma, St Louis, MO, USA), 5 ng/mL murine EGF and 1% of an insulin–transferrin–selenium mix (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) with 5% FetalClone. Cell lines were authenticated and routinely tested for mycoplasma.

Carazo F., Bértolo C., Castilla C., Cendoya X., Campuzano L., Serrano D., Gimeno M., Planes F.J., Pio R., Montuenga L.M, & Rubio A. (2020). DrugSniper, a Tool to Exploit Loss-Of-Function Screens, Identifies CREBBP as a Predictive Biomarker of VOLASERTIB in Small Cell Lung Carcinoma (SCLC). Cancers, 12(7), 1824.

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