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One cycle target labeling kit

Manufactured by Thermo Fisher Scientific
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The One-Cycle Target Labeling Kit is a laboratory tool designed for the preparation of labeled cRNA samples from total RNA for use in gene expression analysis. It enables the conversion of total RNA into labeled cRNA without bias.

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Lab products found in correlation

Genechip scanner 3000, by Thermo Fisher Scientific (1 mentions) Trizol, by Merck Group (1 mentions) Genechip fluidics station 450, by Thermo Fisher Scientific (1 mentions) Eukge ws2v5 450, by Thermo Fisher Scientific (1 mentions) Rneasy minielute kit, by Qiagen (1 mentions) Hybridization oven 640, by Thermo Fisher Scientific (1 mentions) Scanner 3000, by Thermo Fisher Scientific (1 mentions) 3 ivt express kit, by Thermo Fisher Scientific (1 mentions) Bioanalyzer, by Agilent Technologies (1 mentions) H133plus2.0 gene chips, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

GeneChip One-Cycle Target Labeling Kit (6 citations) One-Cycle Target Labeling and Control Reagents Kit (6 citations) One-cycle labeling kit (3 citations) One-Cycle Target Labeling and Control Kit (2 citations)

3 protocols using one cycle target labeling kit

1

Rat Genome 230 2.0 GeneChip analysis

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Total RNA was isolated using Trizol (Sigma) followed by RNeasy Mini Elute Kit (Qiagen, Hilden, Germany) purification. 1 µg of total RNA was used to prepare targets by One-Cycle Target labeling kit (Affymetrix, Santa Clara, CA, USA) following the instructions of the manufacturer. Hybridization cocktails were hybridized onto Rat Genome 230 2.0 GeneChips, containing 31,099 rat gene probes, at 45°C for 17 h (60 Rpm) in a Hybridization Oven 640 (Affymetrix). GeneChips were rinsed and stained in a GeneChip fluidics station 450 using the fluidics protocol “EukGE-WS2v5_450” (Affymetrix). Chips were scanned in a GeneChip scanner 3000 (Affymetrix). For all conditions, three separate experiments were analyzed on separate arrays; that is, a total of 36 arrays were used. Array data is available at Arrayexpress [31 (link)] (https://www.ebi.ac.uk/arrayexpress), accession number E-MTAB-3146.
Prause M., Mayer C.M., Brorsson C., Frederiksen K.S., Billestrup N., Størling J, & Mandrup-Poulsen T. (2016). JNK1 Deficient Insulin-Producing Cells Are Protected against Interleukin-1β-Induced Apoptosis Associated with Abrogated Myc Expression. Journal of Diabetes Research, 2016, 1312705.
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2

Microarray Analysis of Transcriptome

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Total RNA was isolated and quality was checked as previously described (18 (link)). One-Cycle Target Labeling kit or the 3’ IVT Express kit from Affymetrix (Santa Clara, CA) was used following the recommended protocol. Samples were then hybridized to Affymetrix GeneChip Human Genome U133A v2.0 arrays and scanned with a GeneChip Scanner 3000 (GEO accession number (GSE53605).
Maluf D., Dumur C., Suh J., Lee J., Cathro E., King A., Gallon L., Brayman K, & Mas V. (2014). Evaluation of Molecular Profiles in Calcineurin Inhibitor Toxicity Post-Kidney Transplant: Input to Chronic Allograft Dysfunction. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 14(5), 1152-1163.
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3

Transcriptome Profiling of Ovarian Cancer

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Total RNA was isolated from 3 independent cultures of ovarian cancer cells grown on traditional or ultra low attachment 75 cm cell culture flasks in presence of traditional serum-containing media or serum-free stem cell media. Samples were submitted to Laboratory of Molecular Technology, SAIC/NCI-Frederick and RNA quality checked on an Agilent Bioanalyzer; only samples having high quality score (RIN>9) were used for microarray analysis. RNA (1ug) was reverse transcribed with T7-oligo (dT) primers and labeled with biotin using the Affymetrix One Cycle Target Labeling Kit. All replicates of experimental samples were prepared, labeled and hybridized to Affymetrix H133 Plus 2.0 gene chips and scanned on an Affymetrix GeneChip scanner 3000 (Affymetrix, Santa Clara, CA). Data sets are available on the GEO repository (GSE94358).
House C.D., Jordan E., Hernandez L., Ozaki M., James J.M., Kim M., Kruhlak M.J., Batchelor E., Elloumi F., Cam M.C, & Annunziata C.M. (2017). NF-kB promotes ovarian tumorigenesis via classical pathways that support proliferative cancer cells and alternative pathways that support ALDH+ cancer stem-like cells. Cancer research, 77(24), 6927-6940.
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