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31 protocols using hydrochloric acid (hcl)

1

Fly Ash Adsorption of Parabens

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Fly ash was
supplied by Eneva-Energia Pecém, located at São Gonçalo
do Amarante-Ceara, Brazil. The reagents HCl and NaOH and the surfactant
cetyltrimethylammonium bromide (CTAB) were obtained from Vetec, Brazil.
For adsorption tests, individual high-purity standards (≥99%)
of methylparaben (MP), ethylparaben (EP), propylparaben (PP), and
butylparaben (BP) were purchased from Sigma-Aldrich. Their physical–chemical
structures and properties are summarized in Table 1. For chromatographic analysis, methanol
(PanReac AppliChem ITW Reagents, high-performance liquid chromatography
(HPLC) grade) was used. Acetic acid used in the mobile phase was obtained
from Vetec, and NaCl, used in the determination of the point of zero
charge, was obtained from Sigma-Aldrich.
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2

Prussian Blue Staining of Nanoparticles

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Cells were seeded to 24 well culture plates at a seeding density of 10,000 cells/ well and incubated overnight for cell attachment. The medium was then replaced with opti-MEM medium containing 100 ug/mL of nCP:Fe and incubated for 12 hours. Medium was removed and cells were cultured in DMEM complete medium for 6 hours. Cells were then washed with PBS and fixed using 4% paraformaldehyde (Merck Co, India). Cells were then washed and treated with a mixture of 5% potassium ferrocyanide (Sigma-Aldrich Co, USA) and 5% HCl (VeTEC, India) for 10 minutes. Cells were washed and stained with nuclear fast red (Merck- Millipore, USA) for 5 min. Dehydration followed by mounting of coverslips was done. The cells were examined under optical microscope (Olympus, BX5).
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3

Arsenic Speciation Analysis Protocol

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All reagents used in the experiments were of analytical grade and used without further purification. The reagents used were weighed on a Shimadzu analytical scale, model AUY220, to prepare the solutions. The solutions were prepared using ultrapure water (resistivity 18.0 MΩ cm) obtained by the Thermo Scientific Barnstead™ Nanopure™ system (Waltham, MA, USA).
The reagents Na2HAsO4·7H2O (CAS: 10048-95-0) and NaAsO2 (CAS: 7784-46-5) were provided by Sigma-Aldrich (St. Louis, MO, USA), and the reagents NaOH (CAS: 1310-73-2), HNO3 (CAS: 7697-37-2), CH3COOH (CAS: 64-19-7), and HCl (CAS: 7647-01-0) were purchased from Vetec (Brazil). Rhodium (Ra) (CAS: 7440-14-4) and multi-elementary solution were obtained from PerkinElmer (Waltham, MA, USA).
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4

Carbon Synthesis via Glycerol Conversion

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Carbon was produced utilizing glycerol 99% from Vetec (São Paulo, São Paulo), sulfuric acid >98% from LabSynth (Diadema, São Paulo) and acetone from Cosmoquimica Indústria e Comércio (Barueri, São Paulo). Characterization techniques used KBr 99.5% from Sigma-Aldrich (São Paulo, São Paulo), NaOH 99% from Vetec (São Paulo, São Paulo), NaHCO3 99.5% from Sigma-Aldrich (São Paulo, São Paulo), HCl 36.5–38.0% from Vetec (São Paulo, São Paulo). Catalytic tests were performed with D(-)fructose >99,9% from Sigma Aldrich (São Paulo, São Paulo) and Dimethylsulfoxide >99,9% from Synth (Diadema, São Paulo). All chemicals were used as received and without any treatment.
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5

Synthesis of Titanium-Niobium Oxide

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The tetraethylorthosilicate (TEOS, 98%), titanium (IV) butoxide (97%) and niobium (V) pentachloride (99%) were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Ethanol (99.8%), HCl (37% v/v) and HNO3 (95% v/v) were purchased from Vetec (Duque de Caxias, RJ, Brazil). Ultra-pure Milli-Q water was used (resistivity > 18.2 MΩ cm−1, 25 °C, Millipore Milli-Q purification system, Billerica, MA, USA).
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6

Chitosan-Based Metal-Organic Conjugate Synthesis

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Chitosan (medium molecular weight, cat. no. 448877), pyridine-2-carbaldehyde, CuSO4•5H2O, and sodium chloroacetate (98%) were purchased from Sigma-Aldrich. K2Cr2O7, monochloroacetic acid (99%), CH3COONa•3H2O, Na2CO3, anhydrous CaCl2, NaH2PO4, Na2HPO4, and ethylenediaminetetraacetic acid (EDTA) were purchased from Synth (Brazil). Glycine was purchased from Isofar (Brazil). Glacial acetic acid (99.5%) was purchased from Neon (Brazil). Ethyl alcohol (99.8%) was purchased from Dinâmica (Brazil). HCl (37 wt.% in H2O) was purchased from Vetec (Brazil). Filter paper (quantitative, black ribbon, C-41, 12.5 cm diameter, 0.01% ash content, and grammage of 85 g m -2 ) was purchased from Unifil (Brazil).
The degree of acetylation (DA) of the chitosan was determined in a previous study of our research group (Goncalves et al., 2018) (link), using quantitative solid-state 13 C nuclear magnetic resonance (SS 13 C NMR) spectroscopy. The estimated DA value of the chitosan was 33 ± 2%.
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7

Preparation and Modification of Sugarcane Biomass

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CoCl 2 •6H 2 O, CuSO 4 •5H 2 O, monochlroacetic acid (99%), and isopropanol were purchased from Synth (Brazil). Phthalic anhydride (99%) (cat no. 125733) was purchased from Sigma-Aldrich (Brazil). NiCl 2 •6H 2 O, glacial acetic acid (99.5%), NaOH, HCl (37% w/w), acetone, and pyridine (Py) were purchased from Vetec (Brazil). Quantitative filter papers (blue ribbon, JP-41, cat no. 3509-1, 12.5 cm diameter, ash content of 0.00009 g, and grammage of 80 g/cm 2 ) were purchased from JProlab (Brazil). Py was refluxed in a 2 L round-bottomed flask with NaOH pellets for 12 h, distilled, and stored in flat-bottomed flasks containing NaOH pellets before use. All metal-ion solutions were prepared using deionized water (Millipore, model Milli-Q ® Simplicity ® ). SB stalks were collected at Ouro Preto, Minas Gerais, Brazil. SB stalks were prepared for chem-ical modification following the methodology proposed by Ramos et al. (2015) .
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8

Adsorption Studies of Metal Ions

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CoCl 2 Á6H 2 O, CuSO 4 Á5H 2 O, monochloroacetic acid (99%), CH 3 -COONaÁ3H 2 O and isopropanol were purchased from Synth (Brazil). 1,2,4-Benzenetricarboxylic anhydride (Trimellitic anhydride, TA) (97%) (cat no. 552-30-7), Whatman Ò cellulose chromatography papers (20 Â 20 cm) (cat no. WHA3001861) and 3 Å molecular sieve (cat no. 69839) were purchased from Sigma-Aldrich (Brazil). NiCl 2 Á6H 2 O, glacial acetic acid (99.5%), NaOH, HCl (37% w/w), acetone, N,N-dimethylacetamide (DMA) and pyridine (Py) were purchased from Vetec (Brazil). Quantitative filter paper (black ribbon, JP-41, cat no. 3509-1, 12.5 cm diameter, ash content of 0.00009 g and grammage of 80 g cm À2 ) was purchased from JProlab (Brazil). Py was refluxed in a 2 L round-bottom flask with NaOH pellets for 12 h, distilled and stored in flat-bottom flasks containing NaOH pellets before use. Molecular sieve was previously activated in an oven at 150 °C for 2 h. DMA was stored with molecular sieve (3 Å) before use. All metal ion solutions used in the adsorption studies were buffered. Buffers consisting of 0.05 mol L À1 CH 2 ClCOOH/CH 2 ClCOONa (pH from 2.0 to 3.5) and 0.05 mol L À1 CH 3 COOH/CH 3 COONa (pH from 4.0 to 5.5) were prepared in deionized water (Millipore, model Milli-Q Ò ).
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9

Synthesis and Characterization of Metal-Organic Frameworks

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CoCl 2 •6H 2 O, CuSO 4 •5H 2 O, CH 3 COONa•3H 2 O, monochloroacetic acid (99%), and isopropanol were purchased from Synth (Brazil). 1,2,4-Benzenetricarboxylic anhydride (trimellitic anhydride, TA) (97%) (cat no. 552-30-7) and 3 Å molecular sieve (cat no. 208582) were purchased from Sigma-Aldrich (Brazil). NiCl 2 •6H 2 O, glacial acetic acid (99.5%), NaOH, HCl (37% w/w), acetone, N,Ndimetylacetamide (DMA), and pyridine (Py) were purchased from Vetec (Brazil) . Quantitative filter papers (blue ribbon, JP 41, cat no. 3509-1, 12.5 cm diameter, ash content of 0.00009 g, and grammage of 80 g/cm 2 ) were purchased from JProlab (Brazil). Before use, DMA was stored with molecular sieves (3 Å) that had been previously activated in an oven at 150 • C. Py was refluxed in a round-bottomed flask with NaOH pellets for 12 h and distilled before use.
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10

Characterization of Bullfrog Oil Properties

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Bullfrog oil was provided by Asmarana Produtos Naturais (Natal, Brazil). Sodium hydroxide (NaOH), dimethyl sulfoxide (DMSO), potassium hydroxide, diethyl ether, hydrochloric acid (HCl), Tween® 20, sodium thiosulfate, and sodium carbonate were from VETEC (Rio de Janeiro, Brazil). Miglyol® 812 was a gift from Sasol (Witen, Germany). Butylhyldroxytoluene (BHT), buthylhydroxyanisole, potassium biphthalate, potassium dichromate, and Wijs solution were purchased from Labsynth (São Paulo, Brazil). Acetic acid, ethanol P.A, sodium bicarbonate, potassium iodide, starch, and chloroform were from Isofar (Rio de Janeiro, Brazil). Sucralose, Tutti-frutti flavoring and Acesulfame K were purchased from Valdequímica (São Paulo, Brazil). Xanthan gum, sodium benzoate and propylparaben were from ViaFarma (São Paulo, Brazil). Phenolphthalein was provided from Biotec Chemicals (Londrina, Brazil). Mucin Type II (Mucin from porcine stomach) and Span® 80 were from Sigma-Aldrich (São Paulo, Brazil).
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