5 mm tci cryoprobe

Manufactured by Bruker

Sourced in Germany, United States

The 5 mm TCI cryoprobe is a high-performance NMR probe designed for advanced liquid-state NMR experiments. It features a 5 mm sample coil and is optimized for cryogenic operation to enhance sensitivity. The core function of this probe is to provide efficient signal detection and accurate sample temperature control for a variety of NMR applications.

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Lab products found in correlation

Topspin 3, by Bruker (9 mentions) Drx 600 spectrometer, by Bruker (9 mentions) Avance 3 600 mhz spectrometer, by Bruker (6 mentions) 241 polarimeter, by PerkinElmer (5 mentions) Uv 2450 uv vis spectrophotometer, by Shimadzu (5 mentions) Avance 3 500 mhz spectrometer, by Bruker (5 mentions) Avance spectrometer, by Bruker (5 mentions) Avance 3, by Bruker (4 mentions) Acquity uplc beh c18, by Waters Corporation (4 mentions) Bbfo plus smartprobe, by Bruker (4 mentions) Methanol d4, by Merck Group (4 mentions) Topspin, by Bruker (3 mentions) Avance 3 700 mhz spectrometer, by Bruker (3 mentions) Ascend 3 hd 600 mhz spectrometer, by Bruker (2 mentions) Cary 300 spectrophotometer, by Agilent Technologies (2 mentions) Stable isotopically labeled compounds, by Cambridge Isotopes (2 mentions) G2si q tof system, by Waters Corporation (2 mentions) Avance 3 700 spectrometer, by Bruker (2 mentions) Mcp 150 polarimeter, by Anton Paar (2 mentions) Agilent 1200 infinity series hplc, by Agilent Technologies (2 mentions)

Spelling variants of this product

CryoProbe (153 citations) TCI cryoprobe (87 citations) 5 mm cryoprobe (9 citations) 5 mm TCI inverse detection cryoprobe (5 citations) 5 mm TCI triple-resonance cryoprobe (3 citations) 5 mm TCI prodigy cryoprobe (2 citations) 5 mm TCI C/N Prodigy cryoprobe (2 citations)

70 protocols using 5 mm tci cryoprobe

Characterization of Polymer Carboxyl Groups

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NMR spectra were acquired using a High-resolution 500 MHz Bruker NEOn500 Quadruple resonance (H/C/N/2H) equipped with a high-sensitivity TCI 5 mm CryoProbe (Bruker, Billerica, MA, USA) at 25 °C in D₂O and d₆-DMSO.
FT-IR spectra were recorded with a double-beam Perkin Elmer System 2000 Ft-IR Spectrometer (Perkin Elmer, Waltham, MA, USA) in the range of 4500–370 cm⁻¹ using KBr pellets.
The number of terminal carboxyl groups was determined via conductimetric titration. The polymers were dissolved using diluted HCl and the solutions were titrated using a standardized solution of NaOH 0.1 M. The deprotonation of the -COOH end groups resulted in the formation of a small plateau in the conductimetric titration curve. The number of carboxyl groups was determined by the volume difference of added NaOH solution between the initial and the final point of the plateau (Figure S10).

Marsili L., Dal Bo M., Eisele G., Donati I., Berti F, & Toffoli G. (2021). Characterization of Thermoresponsive Poly-N-Vinylcaprolactam Polymers for Biological Applications. Polymers, 13(16), 2639.

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NMR Spectroscopic Characterization Protocol

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Spectra were obtained using a Bruker Avance III 600 MHz instrument equipped with a Bruker TCI 5 mm cryoprobe (Bruker Biospin, Billerica, MA) at 30.0 ± 0.1 °C. ¹H NMR (600 MHz), ¹³C/¹³C (DEPT-135) NMR (150 MHz) and ³¹P/³¹P{¹H} NMR were recorded in CDCl₃. NMR data is reported as follows: chemical shift (δ) (parts per million, ppm); multiplicity: d (doublet), app ten (apparent tentet), qd (quartet of doublets); coupling constants (J) are given in Hertz (Hz). ¹H NMR chemical shifts are calibrated with respect to the residual CHCl₃ singlet centered at 7.26 ppm while for ¹³C NMR the triplet centered at 77.16 ppm from CDCl₃ was used for the spectral calibration.

Subramanian A., Rosales J.A., Leif R.N, & Valdez C.A. (2022). Benzyl trichloroacetimidates as derivatizing agents for phosphonic acids related to nerve agents by EI-GC-MS during OPCW proficiency test scenarios. Scientific Reports, 12, 21299.

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NMR and Mass Spectrometry Characterization

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Nuclear magnetic resonance experiments were performed on a Bruker Ascend III HD 600 MHz spectrometer equipped with a 5 mm cryoprobe -TCI with an effective resolution of 900 MHz (Universidad de Antioquia, Colombia). The mono and bidimensional experiments were stored using the standard pulse sequences of the equipment. Chemical shifts (δ) are expressed in ppm and the data analysis was carried out using MestReNova version 11.0 (Mestrelab Research, Santiago de Compostela, Spain). Mass spectra were obtained in a SQD2 detector coupled to a UPLC H-class chromatographic system in positive and negative modes and data analysis was performed using Waters MassLynx™ software Version 4.1 (Waters, Milford, MA, USA). The elemental composition and high-resolution measurement were carried out on a Q-Exactive hybrid quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA).

Mosquera C., Panay A.J, & Montoya G. (2018). Pentacyclic Triterpenes from Cecropia telenitida Can Function as Inhibitors of 11β-Hydroxysteroid Dehydrogenase Type 1. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(6), 1444.

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NMR Characterization of Organic Compounds

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¹H NMR and ¹³C NMR were recorded on a Bruker Ascend III HD 600 MHz with a 5 mm CryoProbe TCI, using CDCl₃ and D₂O as deuterated solvents; TMS and TSP were employed as the chemical shift reference (δ = 0.0 ppm) and internal standard, respectively. Signals were assigned using one- and two-dimensional ¹H–¹H COSY, ¹H–¹³C HMQC and HMBC spectra.
Non-polar samples solutions were prepared in CDCl₃ and analysed via ¹H NMR with a 30° pulse (Bruker zg30 pulse sequence) and polar protonated derivatives were analysed ¹H NMR with a 90° pulse with water presaturation sequence (Bruker zgpr pulse sequence). All proton spectra were obtained at 298 K, and the delay time was 2 s with eight scans for each spectrum. The spectra were processed using Fourier transform with 64 K data points and a spectral width of 8417.5 Hz.

Linares V., Yarce C.J., Echeverri J.D., Galeano E, & Salamanca C.H. (2019). Relationship between Degree of Polymeric Ionisation and Hydrolytic Degradation of Eudragit® E Polymers under Extreme Acid Conditions. Polymers, 11(6), 1010.

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Comprehensive Analytical Characterization of Compounds

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Optical rotation was recorded using a PerkinElmer 241 polarimeter. UV spectrum was acquired on a Varian Cary 5000 spectrophotometer. IR spectrum was obtained using a Thermo-Nicolet 6700 with Smart iTR™ accessory. 1D and 2D NMR spectra were acquired on Bruker AVII 900 MHz spectrometer equipped with a 5 mm TCI cryoprobe (Supplementary Information S4–19). NMR chemical shifts were referenced to residual solvent peaks (CD₃OD/CD₃OH δ_H 3.31 and δ_C 49.0; Pyr-d₅ δ_H 8.74 and δ_C 150.35). LC-UV-HRMS experiments were performed on a Shimadzu LCMS-IT-TOF equipped with a UPLC C18 column (2.1 × 50 mm x 1.7 μm). Mobile phases consisted of H₂O (A) and CH₃CN (B), both acidified with 0.1% formic acid, flowing at a total rate of 0.5 mL/min. The gradient program was set as follows: 5–100% B for 7 minutes,1-min wash, and 2-min re-equilibration. PDA acquisition ranged from 190 to 450 nm at 4.17 Hz sampling rate. HRMS data were recorded in positive and negative modes from 150 to 3000 m/z. Additional HRMS/MS spectra were acquired at the University of North Carolina at Greensboro using a Thermo QExactive Plus MS (Thermo-Fisher). HRMS/MS spectra were obtained by automatic data-dependent fragmentation with a collision energy of 35 eV.

Crnkovic C.M., Braesel J., Krunic A., Eustáquio A.S, & Orjala J. (2019). Scytodecamide from the Cultured Scytonema sp. UIC 10036 Expands the Chemical and Genetic Diversity of Cyanobactins. Chembiochem : a European journal of chemical biology, 21(6), 845-852.

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Analytical Characterization of Compounds

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Stable isotopically labeled compounds were purchased from Cambridge Isotope Laboratories, Inc. The corresponding unlabeled compounds were purchased from ThermoFisher Scientific. All solvents used for UPLC and HPLC were Optima grade, and water used for chromatography was purified by a Milli-Q water purification system. High resolution MS for pure compounds and metabolomics experiments were performed on a Water Synapt G2Si q-TOF system. NMR spectra were acquired on a Bruker AVANCE III 600 MHz spectrometer, with a 5 mm TCI cryoprobe, and referenced to residual solvent proton and carbon signals. UV spectra were obtained on an Agilent Cary 300 spectrophotometer. Optical rotations were measured on a Perkin Elmer 341 polarimeter.

McCaughey C.S., van Santen J.A., van der Hooft J.J., Medema M.H, & Linington R.G. (2021). An Isotopic Labeling Approach Linking Natural Products with Biosynthetic Gene Clusters. Nature chemical biology, 18(3), 295-304.

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Analytical Characterization of Compounds

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NMR, Optical, and Mass Spectrometry Analysis

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NMR spectra were recorded with an Avance III 700 spectrometer with a 5 mm TCI cryoprobe (¹H 700 MHz, ¹³C 175 MHz) and an Avance III 500 spectrometer (¹H 500 MHz, ¹³C 125 MHz; both Bruker, Billerica, MA/USA). NMR data were referenced to selected chemical shifts of acetone-d₆ (¹H: 2.05 ppm, ¹³C: 29.32 ppm), pyridine-d₅ (¹H: 7.22 ppm, ¹³C: 123.87 ppm) and DMSO-d₆ (¹H: 7.27 ppm, ¹³C: 77.00 ppm), respectively. Optical rotations were taken with a MCP 150 polarimeter (Anton Paar, Graz, Austria); UV spectra were taken with a UV-2450 UV/VIS spectrophotometer (Shimadzu, Kyoto, Japan); ECD spectra were collected with a JD 815 spectrophotometer (Jasco, Pfungstadt, Germany).
ESI-MS spectra were recorded with an UltiMate® 3000 Series uHPLC (Thermo Fisher Scientific, Waltman, MA/USA) utilizing a C18 Acquity® UPLC BEH column (2.1 × 50 mm, 1.7 μm; Waters, Milford, MA/USA) connected to an amaZon® speed ESI Iontrap MS (Bruker). HPLC parameters were set as follows: solvent A: H₂O + 0.1% formic acid, solvent B: acetonitrile + 0.1% formic acid; gradient: 5% B (0.5 min), 5–100% (19.5 min), 100% (5 min), flowrate 0.6 mL/min, and DAD detection 190–600 nm. HR-ESI-MS spectra were obtained with an Agilent 1200 Infinity Series HPLC (Agilent Technologies, Böblingen, Germany; conditions same as for ESI MS spectra) connected to a maXis® ESI-TOF-MS (Bruker).

Gerke J., Köhler A.M., Wennrich J.P., Große V., Shao L., Heinrich A.K., Bode H.B., Chen W., Surup F, & Braus G.H. (2022). Biosynthesis of Antibacterial Iron-Chelating Tropolones in Aspergillus nidulans as Response to Glycopeptide-Producing Streptomycetes. Frontiers in Fungal Biology, 2, 777474.

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1D NOESY NMR Characterization Protocol

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All NMR experiments were performed with a Bruker AVANCE III spectrometer (600.13 MHz) at 298 K with a Bruker 5-mm TCI CryoProbe. Spectra were collected using the software program TopSpin 3.1 (Bruker BioSpin). One-dimensional proton nuclear Overhauser effect spectroscopy with an inverse gated decoupling pulse sequence (noesyigld1 d) using a base opt filter was performed using 64 scans, four dummy scans, and 65536 data points. A relaxation delay of 10 s with a mixing time of 0.01 seconds was used to allow an acquisition time of 14 min for each experiment.

Harnly J., Chen P., Sun J., Huang H., Colson K.L., Yuk J., McCoy J.A., Harbaugh Reynaud D.T., Harrington P.B, & Fletcher E.J. (2015). Comparison of Flow Injection MS, NMR, and DNA Sequencing: Methods for Identification and Authentication of Black Cohosh (Actaea racemosa). Planta medica, 82(3), 250-262.

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Compound Binding to HSP72-NBD

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To confirm the binding of compounds to HSP72-NBD, a series of CPMG and WaterLOGSY experiments were performed. Compounds were assayed at 200 μM in the presence or absence of 10-μM HSP72-NBD. Two hundred micrometers of ATP were also included in the competition experiments. The total assay volume was 200 μL, and the experiments were performed in 3-mm NMR tubes. The buffer was 25-mM Tris, pH 7.5, 50-mM NaCl, 10% D₂O and 100 μM of DTT in deionized water. NMR experiments were conducted at a ¹H frequency of 600 MHz using a Bruker Avance 600 spectrometer (Bruker, Bilerica, MA, USA) equipped with a 5-mm TCI Cryo-probe. All data were acquired and processed using Topspin (Bruker, Bilerica, MA, USA) and MNova (Mestrelab Research SL, Santiago de Compostela, Spain). The relaxation-edited 1H-NMR spectrum was acquired at 298 K using the CPMG sequence with a spin-lock time of 600 ms. The water signal was suppressed using pre-saturation during the relaxation delay (2 s) and by using the Watergate sequence subsequent to the CPMG sequence. For each spectrum, 64 transients were acquired.

O’Connor S., Le Bihan Y.V., Westwood I.M., Liu M., Mak O.W., Zazeri G., Povinelli A.P., Jones A.M., van Montfort R., Reynisson J, & Collins I. (2022). Discovery and Characterization of a Cryptic Secondary Binding Site in the Molecular Chaperone HSP70. Molecules, 27(3), 817.

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