Sorted LSC population was re-suspended in 2:1 Matrigel/Prostate Epithelial growth medium (Matrigel, Cat#.354234, Corning; PrEGM, Cat#.CC-3166, Lonza) in a total volume of 120μL. Cells were seeded at a density of 8,000 or 10,000 cells per well in 12-well plates, and incubated for 7 days. Half medium change was performed every 2–3 days. Numbers of spheres were counted manually at 40x magnification at 7th day under microscope. To examine self-renewal ability of PSCs, spheres from primary culture were released by 1mg/mL Dispase (Cat#.17105-041, Invitrogen) at 37 °C for an hour, and dissociated into single cells for secondary sphere culture. To dissociate intact spheres, collected spheres were incubated with trypsin at room temperature (R.T.) for 10 mins. Trypsinized cells from spheres were passed through 26G needles three times. Dissociated cells were individually plated at 8,000 cells per well density in 12-well plates for 7 days. The numbers of secondary spheres were obtained at 7th day.
Matrigel prostate epithelial growth medium
Manufactured by Corning
Matrigel/Prostate Epithelial growth medium is a cell culture product designed to support the growth and maintenance of human prostate epithelial cells. It provides a suitable environment for the cultivation of these specialized cells.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using matrigel prostate epithelial growth medium
1
Prostate Stem Cell Sphere Culture
2
Prostate Stem Cell Sphere Culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Sorted LSC population was re‐suspended in 2:1 Matrigel/Prostate Epithelial growth medium (Matrigel, Cat#.354234, Corning; PrEGM, Cat#.CC‐3166, Lonza) in a total volume of 120 μl. Cells were seeded at a density of 8,000 or 10,000 cells/well in 12‐well plates, and incubated for 7 days. Half medium change was performed every 2–3 days. Numbers of spheres were counted manually at 40× magnification at 7th day under microscope. To examine self‐renewal ability of PSCs, spheres from primary culture were released by 1 mg/ml Dispase (Cat#. 17105‐041, Invitrogen) at 37°C for an hour, and dissociated into single cells for secondary sphere culture. To dissociate intact spheres, collected spheres were incubated with trypsin at room temperature (R.T.) for 10 min. Trypsinized cells from spheres were passed through 26 G needles three times. Dissociated cells were individually plated at 8,000 cells/well density in 12‐well plates for 7 days. The numbers of secondary spheres were obtained at 7th day.
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