Galangin (purity ≥ 99%) was purchased from Extrasynthese (Genay, France); dimethylsulfoxide (DMSO), Tris–HCl, ethylenediaminetetraacetic acid (EDTA), sodium dodecyl sulphate (SDS), phenylmethylsulfonyl fluoride, bovine serum albumin (BSA), gelatin, leupeptin, Nonidet P-40, deoxycholic acid and sodium orthovanadate were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA); A protein assay kit was obtained from Bio-Rad Labs. (Hercules, CA, USA). Dulbecco’s phosphate buffer solution (PBS), fetal bovine serum (FBS), trypsin-EDTA, and powdered Dulbecco’s modified Eagle’s medium (DMEM) were purchased from Gibco-BRL (Gaithersburg, MD, USA). Matrigel was obtained from BD Transduction Laboratories (San Diego, CA, USA). Antibodies against Akt, ERK1/2, JNK/SAPK, and p38 MAPK, proteins, and phosphorylated proteins were purchased from Cell Signalling Technology (Beverly, MA, USA). An enhanced chemiluminescence (ECL) kit was purchased from Amersham Life Science (Amersham, UK).
Powdered dulbecco s modified eagle s medium dmem
Manufactured by Thermo Fisher Scientific
Sourced in United States
Powdered Dulbecco's modified Eagle's medium (DMEM) is a cell culture medium used to support the growth of various cell lines. It provides a balanced salt solution with amino acids, vitamins, and other nutrients required for cell metabolism and proliferation.
Automatically generated - may contain errors
Lab products found in correlation
Sodium orthovanadate, by Merck Group (1 mentions)
Nonidet p 40, by Merck Group (1 mentions)
Protein assay kit, by Bio-Rad (1 mentions)
Enhanced chemiluminescence ecl kit, by Cytiva (1 mentions)
Sapk jnk, by Cell Signaling Technology (1 mentions)
P38 mapk, by Cell Signaling Technology (1 mentions)
Matrigel, by BD (1 mentions)
Ethylenediaminetetraacetic acid, by Merck Group (1 mentions)
Sodium dodecyl sulphate (sds), by Merck Group (1 mentions)
Leupeptin, by Merck Group (1 mentions)
Tris hcl, by Merck Group (1 mentions)
Phenylmethylsulfonyl fluoride, by Merck Group (1 mentions)
Deoxycholic acid, by Merck Group (1 mentions)
Galangin, by Extrasynthese (1 mentions)
Matrigel matrix, by Corning (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Trypan blue, by Merck Group (1 mentions)
2 protocols using powdered dulbecco s modified eagle s medium dmem
1
Compound Evaluation and Cellular Signaling
2
Phytochemical Extraction and Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Dimethylsulfoxide (DMSO), trypan blue and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2Htetrazolium bromide (MTT) were purchased from Sigma-Aldrich (St. Louis, MO, U.S.A). Phosphate buffered saline was purchased from Merck Millipore Corp (Darmstadt, Germany). Powdered Dulbecco's modified Eagle's medium (DMEM) was purchased from Gibco/BRL (Gaithersburg, MD, U.S.A). Matrigel matrix was purchased from Corning incorporated (Bedford, MA, U.S.A).
Pharmacognosy Journal, Vol 12, Issue 5, Sep-Oct, 2020
Province. Fresh leaves were washed under running tap water and then dried for 36 hours using a hot air oven at 60°C. Dried leaves were crushed and subjected to extraction using the maceration process in methanol for 7 days at temperature. The extracts were collected and concentrated at 40°C under reduced pressure using a rotary evaporator. The dried extracts were stored at 4°C until further use.
Pharmacognosy Journal, Vol 12, Issue 5, Sep-Oct, 2020
Province. Fresh leaves were washed under running tap water and then dried for 36 hours using a hot air oven at 60°C. Dried leaves were crushed and subjected to extraction using the maceration process in methanol for 7 days at temperature. The extracts were collected and concentrated at 40°C under reduced pressure using a rotary evaporator. The dried extracts were stored at 4°C until further use.
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