CDDP, diethyldithiocarbamate (DDTC), 8-cyclopentyl-1,3-dipropylxanthine (DPCPX), dithiothreitol, and sodium deoxycholate were purchased from Sigma–Aldrich (St. Louis, MO, USA). LC–MS/MS grade water, methanol, and acetonitrile were purchased from Fisher Scientific (Pittsburgh, PA, USA). Iohexol was purchased from TCI, Inc. (Portland, OR, USA). Protease inhibitor cocktail was purchased from Thermo Scientific (Rockford, IL, USA). Phenylmethylsulfonyl fluoride (PMSF) was purchased from MP Biomedicals (Solon, OH, USA). Ammonium bicarbonate was purchased from Oakwood Chemical (Estill, SC, USA). Iodoacetamide was purchased from Biorad (Hercules, CA, USA).
8 cyclopentyl 1 3 dipropylxanthine
Manufactured by Merck Group
Sourced in United States
8-cyclopentyl-1,3-dipropylxanthine is a chemical compound used in laboratory research. It functions as an adenosine receptor antagonist. No further details on intended use can be provided.
Automatically generated - may contain errors
Lab products found in correlation
Cyclopentyladenosine, by Merck Group (2 mentions)
Protease inhibitor cocktail, by Thermo Fisher Scientific (2 mentions)
Diethyldithiocarbamate, by Merck Group (1 mentions)
Iodoacetamide, by Bio-Rad (1 mentions)
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
Sodium deoxycholate, by Merck Group (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Lc ms ms grade water, by Thermo Fisher Scientific (1 mentions)
Dithiothreitol (dtt), by Merck Group (1 mentions)
5 n ethylcarboxamidoadenosine neca, by Merck Group (1 mentions)
N methyl β d ribofuranuronamide, by Bio-Techne (1 mentions)
Ib meca, by Bio-Techne (1 mentions)
Amphotericin b, by Merck Group (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
N6 cyclopentyladenosine cpa, by Bio-Techne (1 mentions)
Milli q biocel, by Merck Group (1 mentions)
N6 cyclopentyladenosine, by Merck Group (1 mentions)
Dpcpx, by Merck Group (1 mentions)
7 protocols using 8 cyclopentyl 1 3 dipropylxanthine
1
Proteomic Analysis Reagents and Chemicals
2
Adenosine Receptor Ligand Preparation
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Cyclopentyladenosine (CPA), 5’-(N-ethylcarboxamido)adenosine (NECA), and 8-Cyclopentyl-1,3-dipropylxanthine (DPCPX) were purchased from Sigma-Aldrich, Munich, Germany. 2-[4-(2-carboxyethyl)phenethylamino]-5′-N-ethylcarboxamidoadenosine (CGS-21680), 1-deoxy-1-(6-[([3-lodophenyl]methyl)-amino]-9H-purin-9-yl)-N-methyl-β-D-ribofuranuronamide (IB-MECA) and N-(4-Acetylphenyl)-2-[4-(2,3,6,7-tetrahydro-2,6-dioxo-1, 3-dipropyl-1H-purin-8-yl)phenoxy]acetamide (MRS 1706) were obtained from Tocris (Distributed by BIOZOL Diagnostica, Eching, Germany). Stock solutions (5 mM up to 100mM) were prepared in Ethanol (CPA) or DMSO (all other) and stored at –20°C. Further dilutions were carried out in distilled water.
3
Partial A1 Adenosine Receptor Agonists
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Disposables for tissue culture were from Falcon (Steroglass, Perugia, Italy). Alpha modified Eagle’s minimum essential medium (α-MEM) was purchased from EuroClone S.p.A. (Milan, Italy). L-Glutamine for culture medium, penicillin/streptomycin, amphotericin B, ascorbic acid, dexamethasone were from Sigma-Aldrich (Milan, Italy) as well as CCPA, 8-cyclopentyl-1,3-dipropylxanthine (DPCPX) and all the other chemicals, unless differently indicated. As for the two partial A1R agonists, namely 2′-dCCPA, compound C1, and 3′-dCCPA, compound C2, were synthesized at the University of Camerino (Camerino, Italy). They are derivatives of the A1AR full agonist CCPA (Figure 1 ) and the lack of the hydroxyl groups of the ribose moiety leading the compounds to behave as partial agonists of A1R should be noted, as previously demonstrated [18 (link),19 (link),20 (link)].
4
Pharmacological Modulation of Adenosine Receptors
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All reagents were purchased from Fisher Scientific (Fair Lawn, NJ, USA) unless otherwise stated. Phosphate buffered saline (PBS) was used to calibrate electrodes containing (in mM): 131.25 NaCl, 3.0 KCl, 10.0 NaH2PO4, 1.2 MgCl2, 2.0 Na2SO4, and 1.2 CaCl2 with the pH adjusted to 7.4. Sodium phosphate was purchased from RICCA Chemical Company (Arlington, TX, USA). All aqueous solutions were prepared with deionized water (Milli-Q Biocel; Millipore, Billerica, MA, USA). Adenosine was prepared as a 10 mM stock solution in 0.1 M HClO4 and stored in the refrigerator.
DPCPX (6 mg/kg, i.p., 8-cyclopentyl-1,3-dipropylxanthine, Sigma Aldrich) was dissolved in dimethylsulfoxide (DMSO). CPA (N6-cyclopentyladenosine) was purchased from Tocris Bioscience (Ellisville, MO, USA), dissolved in saline, and administered at 1 mg/kg. These doses were chosen as large doses that were previously used in the literature [12] (link), [16] (link), [17] (link).
DPCPX (6 mg/kg, i.p., 8-cyclopentyl-1,3-dipropylxanthine, Sigma Aldrich) was dissolved in dimethylsulfoxide (DMSO). CPA (N6-cyclopentyladenosine) was purchased from Tocris Bioscience (Ellisville, MO, USA), dissolved in saline, and administered at 1 mg/kg. These doses were chosen as large doses that were previously used in the literature [12] (link), [16] (link), [17] (link).
5
Adenosine Receptor Modulation in Caffeine Exposure
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Drugs used were N6-Cyclopentyladenosine (CPA, a selective adenosine A 1 receptor agonist, 3.35 mg/Kg) and 8-Cyclopentyl-1,3-dipropylxanthine (DPCPX, an adenosine A1 receptor-selective antagonist, 0.8 mg/Kg) were purchased from Sigma-Aldrich, USA. CPA was dissolved in 0.9% saline, DPCPX in 5% dimethyl sulfoxide + 1.25% NaOH 1 M, pH 4.0, administered i.p. in a volume of 1.0 ml/kg. Caffeine 0.3 g/L was diluted in tap water via the drinking water. The dose of caffeine (0.3 g/L) was chosen because it produces blood levels in the dams comparable to those obtained in humans after consumption of ~3 cups of coffee by day (Bona et al., 1995) (link).
6
Dopamine Release Under Adenosine Modulation
7
Pharmacological Compounds for Research
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The drugs hydrogen maleate (MK-801), caffeine, dipyridamole, erythro-9-(2-hydroxy-3-nonyl-adenine) (EHNA), 8-cyclopentyl-1,3dipropylxanthine (DPCPX), cyclopentyladenosine (CPA), S-(4-Nitrobenzyl)-6-thioinosine (NBTI), CGS 21680 hydrochloride hydrate, α,β-methylene adenosine 5′-diphosphate (AMPCP), sulpiride, SCH 23390 hydrochloride and tricaine were purchased from Sigma (St. Louis, MO, USA). ZM 241385 was purchased from Tocris Bioscience (Ellisville, MO, USA).
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