All chemicals were obtained from Sigma-Aldrich (St. Louis, USA). All cell culture solutions and supplements were purchased from Life Technologies Inc. (Gaithersburg, USA). Dulbecco's modified Eagle medium (DMEM) was obtained from Gibco, BRL (Carlsbad, USA). All other high performance liquid chromatography and analytical grade solvents were obtained from SRL (Mumbai, India). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent; reverse transcription polymerase chain reaction (RTPCR) and quantitative real-time polymerase chain reaction (qRT-PCR) reagents; and Lipofectamine 2000 were obtained from Invitrogen (Waltham, USA). Antibodies were purchased from BD biosciences (Gurgaon, India) and Cell Signaling Technologies (Danvers, USA). AG490 and wortmannin were purchased from Merck chemicals (Goa, India). siRNA was purchased from Sigma Aldrich.
Cell culture solutions and supplements
Manufactured by Thermo Fisher Scientific
Sourced in United States
Cell culture solutions and supplements are a range of products designed to support the growth and maintenance of cells in an in vitro environment. These solutions and supplements provide the necessary nutrients, growth factors, and other components required for the optimal cultivation of various cell types, including mammalian, insect, and microbial cells. The core function of these products is to create and maintain a suitable environment for cell culture, ensuring the cells' viability and proliferation.
Automatically generated - may contain errors
Lab products found in correlation
Ag490, by Merck Group (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Wortmannin, by Merck Group (1 mentions)
Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions)
3 isobutyl 1 methyl xanthine (ibmx), by Merck Group (1 mentions)
Rosiglitazone, by Merck Group (1 mentions)
Cgp57380, by Abcam (1 mentions)
Reagents for sds page, by Bio-Rad (1 mentions)
Dexamethasone (dex), by Merck Group (1 mentions)
Insulin, by Merck Group (1 mentions)
Interferon ifn γ, by Thermo Fisher Scientific (1 mentions)
Interleukin il 4, by Thermo Fisher Scientific (1 mentions)
2 protocols using cell culture solutions and supplements
1
Cytotoxicity and Signaling Pathway Analysis
2
Adipogenesis and Macrophage Polarization Protocols
Check if the same lab product or an alternative is used in the 5 most similar protocols
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All cell culture solutions and supplements were purchased from Life Technologies. Reagents for SDS-PAGE were purchased from Bio-Rad. For the adipogenesis experiment, insulin, dexamethasone, IBMX and rosiglitazone were obtained from Sigma. For macrophage polarization, lipopolysaccharide (LPS; L2630-lipopolysaccharides from Escherichia coli 0111:B4) was purchased from Sigma Aldrich, interleukin (IL)-4 and interferon (IFN) γ were purchased from Peprotech. The Mnk inhibitor CGP57380 was obtained from Abcam.
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