Mouse serum

Manufactured by Thermo Fisher Scientific

Sourced in United States

Mouse serum is a biological fluid derived from the blood of laboratory mice. It contains a variety of proteins, hormones, and other biomolecules that are naturally present in the mouse circulatory system.

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Lab products found in correlation

Cnbr activated sepharose, by GE Healthcare (3 mentions) Dy285b, by R&D Systems (2 mentions) Lsrfortessa, by BD (2 mentions) Lsr 2 flow cytometer, by BD (2 mentions) Horse serum, by Vector Laboratories (2 mentions) Goat serum, by Atlanta Biologicals (2 mentions) Rat anti mouse fcγiii 2 mab 2.4g2, by BD (2 mentions) Cd38 90 pe, by Thermo Fisher Scientific (2 mentions) Bovine serum albumin (bsa), by Merck Group (2 mentions) Lsr fortessa cytometer, by BD (1 mentions) Golgiplug, by BD (1 mentions) Paraformaldehyde (pfa), by Merck Group (1 mentions) Monensin, by Thermo Fisher Scientific (1 mentions) Anti cd3, by BioLegend (1 mentions) Anti cd40l, by Thermo Fisher Scientific (1 mentions) Prism 8, by GraphPad (1 mentions) Ack lysis buffer, by Thermo Fisher Scientific (1 mentions) Ficoll hypaque, by GE Healthcare (1 mentions) Heat inactivated, by Thermo Fisher Scientific (1 mentions) Facscanto instrument, by BD (1 mentions)

Spelling variants of this product

Normal mouse serum (35 citations) Donkey serum anti-mouse IgG (2 citations) Control pooled mouse serum (2 citations) Rat and mouse serum (2 citations) Mouse anti-S serum (1 citations)

31 protocols using mouse serum

Stability Assessment of MCL Analogs

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To assess the stability of MCL analogs, free compounds (5 μg) were added to PBS (pH 7.4), 100% mouse serum (Life Technologies, Cat #: 10 401), or 1:1 [100% mouse serum]/[PBS] in room temperature. Additionally, drug-loaded NPs in ddH₂O was added to mouse serum (1:1/[100% serum]). Samples were extracted twice with ethyl acetate (300 μL) by centrifugation at 11 000 rpm for 2 mins. Combined supernatants were dried under a nitrogen stream, dissolved in methanol (200 μL), filtered using 0.22 μm PTFE filters, and then analyzed via HPLC as described previously.

Ackun-Farmmer M.A., Alwaseem H., Counts M., Bortz A., Giovani S., Frisch B.J., Fasan R, & Benoit D.S. (2021). Nanoparticle-Mediated Delivery of Micheliolide Analogs to Eliminate Leukemic Stem Cells in the Bone Marrow. Advanced therapeutics, 5(1), 2100100.

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Stability Profiling of Drug Candidates

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Compounds 1-4 were incubated at 37 °C in a shaking incubator as PBS solutions (pH 7.4; 100 μg/ml). Aliquots (200 μl) were collected at subsequent time points (0, 15 min, 1 h, 3 h, 6 h, 24 h, 96 h) and frozen at -20 °C. The samples were analyzed in triplicate by LC-MS. Peaks relative to the pro-drugs were integrated and area values from samples at different time points were used to obtain fractions of intact test compounds. Fraction values were plotted to obtain stability profiles showed in figure 2.
Analogously, test compounds were dissolved in DMSO (10 μl), diluted with freshly thawed mouse serum (Invitrogen) (100 μg/ml) and incubated at 37 °C in a shaking incubator. Aliquots (100 µl) were collected at subsequent time points (0, 20 min, 1 h, 3 h, 6 h, 24 h and 48 h) and frozen at -20°C. Samples were thawed and diluted with four volumes of MeOH (400 μl). After vigorous vortex agitation for 1 min, the protein precipitate was spun down and 400 μl of the supernatant was lyophilized overnight. The resulting solid material was re-dissolved in 200 µl of water, filtered and analyzed as described above.

Cazzamalli S., Corso A.D, & Neri D. (2016). Linker stability influences the anti-tumor activity of acetazolamide-drug conjugates for the therapy of renal cell carcinoma. Journal of controlled release : official journal of the Controlled Release Society, 246, 39-45.

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Multiparametric Immune Cell Analysis

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PBMCs, monocytes, MDMs, and LPMCs were stained in PBS with 1% FCS and 0.1% sodium azide (FACS buffer) by first preincubating in 2% mouse serum (Invitrogen), followed by staining for 20 min on ice (see Table S1 for details of all antibodies used). Cells were analyzed using a LSR Fortessa cytometer (BD Biosciences). Data were analyzed using Flowjo v10.1 (TreeStar).

Kelly A., Gunaltay S., McEntee C.P., Shuttleworth E.E., Smedley C., Houston S.A., Fenton T.M., Levison S., Mann E.R, & Travis M.A. (2018). Human monocytes and macrophages regulate immune tolerance via integrin αvβ8–mediated TGFβ activation. The Journal of Experimental Medicine, 215(11), 2725-2736.

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In Vitro Stability Evaluation of ADCs

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ADCs were incubated at a concentration of 150 μg/mL in mouse serum (Invitrogen) at 37 °C in a shaking incubator. At various time points, aliquots were purified by affinity chromatography onto an antigen-coated resin, based on 11-EDA-12 coupled to CNBr-activated Sepharose (GE Healthcare), washed with PBS and then eluted with 0.1 M glycine solution (pH = 3), prior to analysis by SDS-PAGE and mass spectrometry. In the case of SIP(F8)-SS-DM1, the relative concentration of ADC was assessed by ESI-MS using SIP(F8) coupled to iodoacetamide as internal standard. For IgG(F8)-SS-DM1, the relative ratios of mass spectrometry peak intensity for the unmodified and the drug-modified light chain were used for the quantification of drug release rates [Figure 4].

Gébleux R., Wulhfard S., Casi G, & Neri D. (2015). Antibody format and drug release rate determine the therapeutic activity of non-internalizing antibody-drug conjugates. Molecular cancer therapeutics, 14(11), 2606-2612.

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Activated PBMC cytokine profiling

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Banked frozen responder PBMCs were thawed and rested overnight. Responder PBMCs were stimulated with donor cell lysate at 1:5 ratio for 6 hours in the presence of Golgi-plug (BD) and Monensin (eBioscience) at 37 °C, 5% CO₂. Cells were then washed, and surface stained with anti-CD3 (Biolegend, San Diego, CA), -CD8, -CXCR5, (BD) for 20 minutes at 4 ^οC, washed, and fixed with 1% paraformaldehyde (Sigma, St. Louis, MO) for 40 minutes. Subsequently, the cells were permeabilized and incubated with mouse serum (Invitrogen, Frederick, MD) for 5 minutes at room temperature, followed by anti-CD40L (eBioscience) and IL-21 monclonal Abs (BD) for 30 minutes at room temperature. Cell were washed and immediately acquired as described previously.

Macedo C., Hadi K., Walters J., Elinoff B., Marrari M., Zeevi A., Ramaswami B., Chalasani G., Landsittel D., Shields A., Alloway R., Lakkis F.G., Woodle E.S, & Metes D. (2018). Impact of Induction Therapy on Circulating T Follicular Helper Cells and Subsequent Donor-Specific Antibody Formation After Kidney Transplant. Kidney International Reports, 4(3), 455-469.

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Serum-mediated Decay of mLiTCo Binding

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Purified mLiTCo and mLiTCo-Albu were incubated in mouse serum (Invitrogen) at 37°C, for at least 7 days. The binding activity of the sample at 0 h was set as 100% in order to calculate the time corresponding to percentage of decay in binding activity. Samples were analyzed with Thermo Scientific Multiskan FC photometer and GraphPad Prism 8.4.0 software.

Hangiu O., Compte M., Dinesen A., Navarro R., Tapia-Galisteo A., Mandrup O.A., Erce-Llamazares A., Lázaro-Gorines R., Nehme-Álvarez D., Domínguez-Alonso C., Harwood S.L., Alfonso C., Blanco B., Rubio-Pérez L., Jiménez-Reinoso A., Díez-Alonso L., Blanco F.J., Sanz L., Howard K.A, & Álvarez-Vallina L. (2022). Tumor targeted 4-1BB agonist antibody-albumin fusions with high affinity to FcRn induce anti-tumor immunity without toxicity. iScience, 25(9), 104958.

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Allogeneic Blood WBC Characterization

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DCreg were spiked into a set volume of allogeneic whole blood from a healthy control equivalent to 1:10 ratio to white blood cells (WBC) and incubated for 18 h at 37C and 5% CO₂. Red blood cells (RBC) were then lysed using ACK lysis buffer (Gibco) and the WBC blocked with mouse serum (Invitrogen) and stained with mAb, including HLA, as described for flow cytometry (FC) analysis.

Macedo C., Tran L.M., Zahorchak A.F., Dai H., Gu X., Ravichandran R., Mohanakumar T., Elinoff B., Zeevi A., Styn M.A., Humar A., Lakkis F.G., Metes D.M, & Thomson A.W. (2020). Donor-derived regulatory dendritic cell infusion results in host cell cross-dressing and T cell subset changes in prospective living donor liver transplant recipients. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 21(7), 2372-2386.

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Monocyte MR Expression in Alcoholic Hepatitis

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To investigate whether peripheral monocytes express MR and therefore could be a source of sMR, we studied the MR expression using flow cytometry. 20 patients with AH, 10 patients with AC, and 10 HCs was studied. Peripheral blood mononuclear cells were isolated by Ficoll-Hypaque (GE Healthcare Bio-sciences, Uppsala, Sweden) from EDTA whole blood samples. Samples were stored at -140°C until en bloc analyses. Cells were thawed in PBS containing 20% heat-inactivated pooled human AB-serum and blocked with 10 μl heat-inactivated mouse serum (Invitrogen, Carlsbad, California, USA) before staining with optimized amounts of fluorescent-conjugated antibodies; CD14-APC (clone MFP9; BD Bioscience, San Diego, CA), CD206-FITC (clone 19.2; BD Bioscience, San Diego, CA). Relevant isotype controls were included. The samples were analysed within 24 hours of preparation using a FACSCanto instrument (BD Bioscience). Data analyses were carried out using FlowJo v10.1 (Tree Star Inc., Ashland, OR). We report the relative fluorescent intensity (MFI) of sMR on the CD14+ monocytes.

Sandahl T.D., Støy S.H., Laursen T.L., Rødgaard-Hansen S., Møller H.J., Møller S., Vilstrup H, & Grønbæk H. (2017). The soluble mannose receptor (sMR) is elevated in alcoholic liver disease and associated with disease severity, portal hypertension, and mortality in cirrhosis patients. PLoS ONE, 12(12), e0189345.

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ELISA-based IFN-γ quantification

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Plasma collected from blood samples with or without S1 peptide stimulation was assessed for interferon-γ (IFN-γ) content by ELISA (DY285B, R&D systems) according to the manufacturer’s instructions. To minimize non-specific reactivity, plasma was diluted (1:2) in PBS containing 1% BSA and 10% mouse serum (Invitrogen). Plates were analysed for optical densities at 450 nm and 570 nm using a FLUOstar Omega plate reader (BMG, Ortenberg, Germany). Levels of IFN-γ induced in response to S1 peptides, with background IFN-γ production in unstimulated samples subtracted, are presented throughout the manuscript. The limit of detection (LOD) of the assay was 10 pg/ml as reported elsewhere,¹³ (link) and thus this threshold was used in the study.

Al-Dury S., Waern J., Waldenström J., Alavanja M., Saed H.H., Törnell A., Arabpour M., Wiktorin H.G., Einarsdottir S., Ringlander J., Ringström G., Hellstrand K., Martner A, & Lagging M. (2022). Impaired SARS-CoV-2-specific T-cell reactivity in patients with cirrhosis following mRNA COVID-19 vaccination. JHEP Reports, 4(7), 100496.

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CTC Detection to Evaluate Anti-miR-31 Therapy

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The described animal protocols were reviewed and approved by Dartmouth's Institutional Animal Care and Use Committee (IACUC). To compare the number of CTC in mouse blood from wild-type FVB mice and transgenic CEO mice that develop spontaneous lung tumors, six to seven mice of each arm were used. The following study was then performed to compare the number of CTC in mouse blood in correlation to lung pathology using anti-miR-31 LNA as a novel therapeutic strategy. Seven- month old, sex-matched CEO mice were each injected with either vehicle (PBS with 10% mouse serum (Invitrogen, Carlsbad, CA)), control LNA (3 mg/ml), or anti-miR-31 LNA (3 mg/ml) via tail veins once daily for 5 days before being euthanized using an IACUC-approved protocol at 24 hrs after the last treatment. Note that three mice treated with vehicle were 17 months old. Five to six mice of each treatment group were used. Blood for CTC detection was drawn via cardiac puncture and lung tissues were harvested, formalin-fixed and paraffin-embedded, processed and sectioned for histopathology and hematoxylin and eosin (H&E) staining. The pathologist was blinded to the treatment administered.

Myung J.H., Roengvoraphoj M., Tam K.A., Ma T., Memoli V.A., Dmitrovsky E., Freemantle S.J, & Hong S. (2015). Effective Capture of Circulating Tumor Cells from a Transgenic Mouse Lung Cancer Model using Dendrimer Surfaces Immobilized with anti-EGFR. Analytical chemistry, 87(19), 10096-10102.

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