3h palmitate

Manufactured by American Radiolabeled Chemicals

Sourced in United States

3H-palmitate is a radioactively labeled fatty acid compound. It is commonly used in research applications to study lipid metabolism and transport processes.

Automatically generated - may contain errors

Lab products found in correlation

Typhoon trio, by GE Healthcare (2 mentions) Namp100, by GE Healthcare (2 mentions) Amersham hyperfilm mp, by GE Healthcare (1 mentions)

2 protocols using 3h palmitate

Radioactive Fatty Acid Labeling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were seeded in tissue culture dishes as described above. For labelling, the cells were starved using IM medium (Glasgow minimal essential medium buffered with 10 mM Hepes, pH 7.4). After 1 h, the medium was replaced by IM with 3H-palmitate at 200 µCi/mL (American Radiolabeled Chemicals, US) for 2 h at 37 °C. Cell lysis, immunoprecipitation and SDS-PAGE were performed as above. The gels were fixed for 30 min with 10% acetic acid, 25% isopropanol in water and the signal was amplified for 30 min with NAMP100 (GE Healthcare, US). The gels were then dried and applied to an Amersham Hyperfilm MP (GE Healthcare, US). The radioactivity was visualized and quantify using a Typhoon TRIO (GE Healthcare, US).

Sandoz P.A., Denhardt-Eriksson R.A., Abrami L., Abriata L.A., Spreemann G., Maclachlan C., Ho S., Kunz B., Hess K., Knott G., S. Mesquita F., Hatzimanikatis V, & van der Goot F.G. (2023). Dynamics of CLIMP-63 S-acylation control ER morphology. Nature Communications, 14, 264.

+ Open protocol

+ Expand

Radioactive Protein Labeling and Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were in tissue culture dishes as described above. For labelling, the cells were starved using IM medium (Glasgow minimal essential medium buffered with 10 mM Hepes, pH 7.4). After 1 h, the medium was replaced by IM with 3H-palmitate at 200 µCi/mL (American Radiolabeled Chemicals, US) for 2 h at 37°C. Cell lysis, immunoprecipitation and SDS-PAGE were performed as above. The gels were xed for 30 min with 10% acetic acid, 25% isopropanol in water and the signal was ampli ed for 30 min with NAMP100 (GE Healthcare, US). The gels were then dried and applied to an Amersham Hyper lm MP (GE Healthcare, US). The radioactivity was visualized and quantify using a Typhoon TRIO (GE Healthcare, US). 35

Sandoz P.A., Denhardt-Eriksson R., Abrami L., Abriata L.A., Spreemann G., Maclachlan C., S H., Kunz B., Hess K., Knott G., Mesquita F.S., Hatzimanikatis V., & Goot F.G.v.d. (2022). Model-driven understanding of CLIMP-63 structure and S-acylation reveals fine tuning of ER morphology.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!