Digital images were adjusted for brightness and contrast with Adobe® Photoshop® CS6 and compiled with Adobe® InDesign® CS6 on a MacBook Pro.
Indesign cs6
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Lab products found in correlation
Nis elements microscope imaging software, by Nikon (4 mentions)
Ds f2, by Nikon (3 mentions)
Eclipse 80i microscope, by Nikon (2 mentions)
Jsm 6610lv, by JEOL (2 mentions)
Illustrator cs6, by Adobe (2 mentions)
Smz25, by Nikon (1 mentions)
Jsm 6335f, by JEOL (1 mentions)
Ds ri2, by Nikon (1 mentions)
Em scd 500, by Leica (1 mentions)
Ds ri2 camera, by Nikon (1 mentions)
Eclipse ni compound microscope, by Nikon (1 mentions)
Em scd500, by JEOL (1 mentions)
Dsm 940a, by Zeiss (1 mentions)
Edwards sputter coater, by Edwards Lifesciences (1 mentions)
Stemi 305, by Zeiss (1 mentions)
Bx61 microscope, by Olympus (1 mentions)
Analysis five software, by Olympus (1 mentions)
15 protocols using indesign cs6
1
Photoshop and InDesign Image Editing
2
Taxonomic Study of Haplocookia mauritanica
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The material of the new species was collected by NA, stored in 70% ethanol, and deposited in the Muséum national d’Histoire naturelle (MNHN Natural History Museum of Denmark, Zoological Museum – University of Copenhagen (ZMUC Naturhistorisches Museum Wien (NHMW Haplocookia mauritanica MNHN NHMW with a Nikon DS-F2.5 camera mounted on a Nikon SMZ25 stereomicroscope, using NIS-Elements Microscope Imaging Software with an Extended Depth of Focus (EDF ) patch. All images were processed with Adobe Photoshop CS6 and assembled in Adobe InDesign CS6.
3
Detailed Protocols for Specimen Collection and Imaging
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All specimens were collected by hand and preserved in 70% or 96% ethanol. The holotype was photographed in situ using a Canon 400D camera with a 65 mm macro objective. Microphotographs were obtained with a Nikon DS-F2.5 camera mounted on a Nikon SMZ25 stereomicroscope using NIS-Elements Microscope Imaging Software with an Extended Depth of Focus (EDF ) patch. For scanning electron microscopy, parts of some specimens were cleaned with ultrasound, transferred to 96% ethanol then to acetone, air-dried, mounted on aluminum stubs, coated with Platinum/Palladium and studied in a JEOL JSM-6335F scanning electron microscope. All images were edited in Adobe Photoshop CS6 and assembled in Adobe InDesign CS6. Type material is shared between the Croatian Biospeleological Society – Croatian Natural History Museum (CBSS ), The Natural History Museum Denmark – University of Copenhagen (ZMUC ), Naturhistorisches Museum Wien (NHMW ), and the National Museum of Natural History, Sofia (NMNHS ).
Morphological terminology follows Bonato et al. (2010) .
Morphological terminology follows Bonato et al. (2010) .
4
Brightfield Microscopy Image Processing
5
Imaging and Processing Embryo Samples
6
Detailed Photographic Catalog of Millipede Morphology
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The material was obtained from different museums (see list of repositories), studied and photographed using a Nikon DS-Ri2 camera mounted on a Nikon SMZ25 stereomicroscope, using NIS-Elements Microscope Imaging Software with an Extended Depth of Focus (EDF ). Obtained images were edited in Adobe Photoshop CS6 and assembled in Adobe InDesign CS6. The map is performed used QGIS 3.28.9 (Firenze) with Open Topography-DEM-Downloader 2.0 plugin and WGS 84.
Acronyms of the repositories:
IZCAS National Zoological Museum of China, Institute of Zoology, Chinese Academy of Sciences , Beijing, China.
NMNHS National Museum of Natural History, Bulgarian Academy of Sciences , Sofia, Bulgaria.
ZMUM
List of abbreviations:
a lobe-like mesal process of coxa
b (sub-)falcate mesal process of coxa
c coxa
Ca
Cl
dl
dp
k lateral process of the distal part of telopodite
M mesal process of the distal part of telopodite
n notch on the distal part of the telopodite
p lateral process of the proximal part of telopodite
pf1
pf2
pr
ps
PT
s solenomere
T telopodite
tb
Tp
tr
Acronyms of the repositories:
List of abbreviations:
7
Efficient Illustration and Layout Protocol
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Schematics, tables, and vector graphics were created using Adobe Illustrator CS6, and figures are arranged and compiled using Adobe InDesign CS6.
8
Microscopic Examination of Arthropod Mouthparts
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The mandibles and/or first maxillae were detached from the head with forceps and dissecting needles in one to seven adult male or female individuals per species (see Table 1 ; Supporting Information 1 ). Manual multifocus images of the sclerotized parts of the mandibles and first maxillae were obtained with a Nikon Eclipse Ni compound microscope (LM) equipped with a Nikon DS‐Ri2 camera using NIS‐Elements Microscopic Imaging Software. For scanning electron microscopy (SEM), the specimens were: (a) cleaned in an ultrasonic bath (50–60 Hz) for 5 to 10 s (maximum); (b) dehydrated in an ascending alcohol series (70%, 80%, 90%, 96% EtOH, 2 × 10 min each); (c) covered with HMDS (hexamethyldisilazane) and air dried overnight. The mouthparts were mounted on sticky aluminium tape fixed on aluminium stubs, glued with conductive silver and coated with platinum (Leica EM SCD500). Then, they were studied and photographed with a JEOL JSM 6610‐LV at an accelerating voltage of 10–15 kV. LM and some SEM images were stacked and processed using Zerene Stacker (version 1.04) and Adobe Photoshop CS6. Figures were assembled in Adobe InDesign CS6.
Terminology follows Bonato et al. (2010 ), Edgecombe et al. (2002 ), and Edgecombe et al. (2003 ) where applicable.
Terminology follows Bonato et al. (
9
Epipharynx and Hypopharynx Dissection Procedure
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The epipharynx and hypopharynx were dissected from the preoral chamber as described in Koch and Edgecombe (2008) (link) in one to four adult male or female individuals per species. Multifocus images of the sclerotized parts of the epipharynx and hypopharynx were obtained with a Nikon SMZ25 stereomicroscope equipped with a Nikon DS-F2.5 camera using NIS-Elements Microscope Imaging Software with an PageBreakExtended Depth of Focus (EDF ) patch. For scanning electron microscopy (SEM ), the specimens were: (1) cleaned in an ultrasonic bath (50–60 Hz) for 5 to 10 seconds (maximum), occasionally in a solution of 15% hydrogen peroxide for 2 hours; (2) dehydrated in an ascending alcohol series (70%, 80%, 90%, 96% EtOH, 2 × 10-15 min each); (3) air dried overnight (or covered with HMDS) or critical point dried (Leica 300 CPD). Specimens were mounted on aluminium stubs equipped with a sticky aluminium tape, glued with conductive silver, coated with platinum (Leica EM SCD500) and studied with a JEOL JSM 6610-LV at an accelerating voltage of 15 kV. Figures were processed with Adobe Photoshop CS6 and assembled in Adobe InDesign CS6.
Terminology follows Koch and Edgecombe (2008) (link).
Terminology follows Koch and Edgecombe (2008) (link).
10
Stereomicroscope Imaging of Insect Specimens
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All specimens were collected by hand or obtained by sifting in October 2017 and kept in 96% ethanol in the collections of the Naturhistorisches Museum Wien. Multifocus images were obtained with a Nikon SMZ25 stereomicroscope equipped with a Nikon DS-F2.5 camera using NIS-Elements Microscope Imaging Software with an Extended Depth of Focus (EDF) patch. Some images were edited in Photoshop CS6 and figures assembled in Adobe InDesign CS6. The map was produced in QGIS 2.18.19, using the coordinate reference system WGS84 and topographic data obtained from USGS (2006) Shuttle Radar Topography Mission, 3 Arc Second scene SRTM_f03_p208r040, Filled Finished-A 2.0, Global Landcover Facility, University of Maryland, College Park, Maryland, February 2000.
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