The largest database of trusted experimental protocols

Automated elisa reader

Manufactured by Molecular Devices
Sourced in China

The Automated ELISA Reader is a laboratory instrument designed to measure and analyze Enzyme-Linked Immunosorbent Assay (ELISA) results. It automates the process of reading and interpreting the colored reactions generated in ELISA plates, providing quantitative data on the target analytes present in the samples.

Automatically generated - may contain errors

3 protocols using automated elisa reader

1

ELISA for Serum IgG and Mucosal IgA

Check if the same lab product or an alternative is used in the 5 most similar protocols
Specific IgG in serum and IgA in mucosal suspensions were determined by enzyme-linked immunosorbent assay (ELISA) as previously described17 . In brief, ELISA plates were coated with the purified recombinant HA protein (our laboratory saved) in carbonate buffer (pH = 9.6) at 4 °C overnight. Plates were saturated with PBS containing 1% BSA at 37 °C for 2 h. The serum and mucosal suspensions of dilution degrees were added and incubated for 1 h at 37 °C. Subsequently, the HRP-conjugated goat anti-chicken IgG and goat anti-chicken IgA (BETHYL, CA) were added to the wells and incubated for an additional 1 h at 37 °C. Subsequently, a substrate solution containing o-phenylenediamine (OPD) and H2O2 was added, the reaction was allowed to proceed for 15 min at room temperature before it was terminated by the stop solution. Finally, the absorbance was measured at 450 nm, using an automated ELISA reader (Molecular Devices, Shanghai, CA).
+ Open protocol
+ Expand
2

Quantifying Mouse Serum Immunoglobulins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total serum IgG levels were measured by ELISA as previously described [19 ]. In brief, 96-well PVDF plates were coated with anti-mouse IgG at 10μg/ml in 1x PBS. Serum samples were added to plates blocked with 1% BSA in 1x PBS. Plates were then washed and incubated with alkaline phosphatase-conjugated goat anti-mouse IgG (Jackson ImmunoResearch Laboratories, West Grove, PA). The plates were read with an automated ELISA reader (Molecular Devices, Sunnyvale, CA). Total IgE levels were determined with the BD OptEIA mouse IgE ELISA Set (BD Biosciences, San Jose, CA). For isotype-specific anti-goat Ab levels, plates were coated with goat IgG (10μg/ml) and binding detected with AP-conjugated anti-mouse immunoglobulin isotype Abs.
+ Open protocol
+ Expand
3

PEDV Antibody Detection by ELISA

Check if the same lab product or an alternative is used in the 5 most similar protocols
Nonspecific SIgA and PEDV-specific IgG and SIgA were measured by ELISA according to the instructions as previously described [26 ]. Briefly, the ELISA plates were first coated with PEDV COE protein at 4 °C overnight and saturated with PBS (1% BSA) at 37 °C for 2 h. Second, the mucosal suspension and serum were added and incubated for another 1 h at 37 °C. Subsequently, HRP-conjugated goat anti-mouse IgG and goat anti-mouse IgA were added and incubated at 37 °C for 1 h. Third, a substrate solution containing H2O2 and o-phenylenediamine was added at room temperature for 15 min before termination. Finally, we used an automated ELISA reader (Molecular Devices, Shanghai, China) to detect the absorbance at OD450 nm.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!