The α- and β-synucleins were identified by mouse monoclonal antibody purchased from Santa Cruz, Inc. (Dallas, USA): α/β-synuclein (F-11, sc-514908, 1:500 dilution). glyceraldehyde-3P-dehydrogenase (GAPDH)- and actin-oriented antibodies were also from Santa Cruz, Inc. (Dallas, USA): GAPDH (FL-335, sc-25778, 1:5000 dilution), actin (I-19, sc-1616, 1:2500 dilution). Beta-actin polyclonal antibody was purchased from Bioss Antibodies: β-actin (bs-0061R, 1:5000 dilution). Sheep anti-mouse IgG-HRP (NA931V, 1:10000 dilution) was from GE Healthcare UK and goat anti-rabbit IgG-HRP (sc-2004, 1:10000 dilution) was from Santa Cruz, Inc. (Dallas, USA).
β actin bs 0061r
Manufactured by Bioss Antibodies
Sourced in China, United States
β-actin (bs-0061R) is a primary antibody product offered by Bioss Antibodies. It is a polyclonal antibody that recognizes the β-actin protein, which is a ubiquitous and highly conserved cytoskeletal protein found in all eukaryotic cells. This antibody can be used for various applications, such as western blotting, immunohistochemistry, and immunocytochemistry, to detect and analyze the expression of β-actin in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Pvdf membrane, by Merck Group (2 mentions)
Bs 0295m hrp, by Bioss Antibodies (2 mentions)
Recombinant mouse rankl, by R&D Systems (2 mentions)
Hek293 cell line, by American Type Culture Collection (2 mentions)
Raw264.7 cell line, by American Type Culture Collection (2 mentions)
M csf, by R&D Systems (2 mentions)
Goat anti rabbit igg hrp sc 2004, by Santa Cruz Biotechnology (1 mentions)
Beta actin, by Bioss Antibodies (1 mentions)
Gapdh fl 335 sc 25778, by Santa Cruz Biotechnology (1 mentions)
Dl dithiothreitol, by Solarbio (1 mentions)
Ripa buffer, by Beyotime (1 mentions)
Ecl reagent, by Beyotime (1 mentions)
Sds page, by Solarbio (1 mentions)
Mmp9 bs 4593r, by Bioss Antibodies (1 mentions)
Polyvinylidene difluoride membrane, by Cytiva (1 mentions)
Bca protein quantification kit, by Vazyme (1 mentions)
Chemiluminescent substrate kit, by Merck Group (1 mentions)
Sodium dodecyl sulfonate polyacrylamide gel, by Solarbio (1 mentions)
Ripa buffer, by CWBIO (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
16 protocols using β actin bs 0061r
1
Immunoblotting of Synuclein Proteins
2
Comprehensive Molecular Reagents Protocol
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CA (C110084) was purchased from Aladdin (Shanghai, China). 3-Phenylpropanal (BD17390) was purchased from Bidepharm (Shanghai, China). Cinnamic acid (C804991) was obtained from Macklin (Shanghai, China). FTM (S87283) and TAX (B21695) were purchased from Yuanye (Shanghai, China). DITC (F21540) was purchased from MERYER (Shanghai, China). Pt (HY-17394) was purchased from MedChemExpress (MCE, Monmouth, NJ, USA). TMZ (AK-72945) was purchased from Ark Pharm (Chicago, IL, USA). D,L-dithiothreitol was purchased from Solarbio (DTT, A285, Beijing, China). AP-III-a4 (HY-15858A) was purchased from MCE. Antibodies against Bax (50599-2-lg), Bcl2 (60178-1-lg), and Bid (10988-1-AP) were obtained from Proteintech (Chicago, IL, USA); ENO1 (bs-3978R), anti-GADPH (bsm-0978M), and β-actin (bs-0061R) antibodies were purchased from Bioss (Beijing, China). Alexa-Fluor-594-conjugated goat anti-rabbit IgG (ab150084) was purchased from Abcam (Cambridge, MA, UK), and a goat anti-rabbit IgG (#7074) secondary antibody was obtained from CST (Beverly, MA, USA).
3
Western Blot Protein Analysis
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The protein was contained by lysing tissues and cells into RIPA buffer (Beyotime), electrophoresed on SDS-PAGE (Solarbio, Beijing, China) and then blotted onto PVDF membranes (Millipore, Billerica, MA, USA). After blockage in 5% skim milk for 1 h, the proteins were interacted overnight with primary antibodies and secondary antibody (bs-0295M-HRP; Bioss, Beijing, China) for 1.5 h. The bands were visualized with ECL reagent (Beyotime) and band intensity was examined with ImageJ (NIH, Bethesda, MD, USA). The primary antibodies including β-actin (bs-0061R; Bioss), hexokinase2 (HK-2; bs-3993R; Bioss), CyclinD1 (bs-0623R; Bioss), MMP9 (bs-4593R; Bioss), or KLF12 (bs-16783R; Bioss)
4
Protein Quantification and Western Blot
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The isolation of total protein was executed utilizing RIPA buffer (CWBio, Beijing, China) and the concentrations of proteins were measured with a BCA Protein Quantification Kit (Vazyme, Nanjing, China). Then equal amounts of protein were separated through sodium dodecyl sulfonate-polyacrylamide gel (Solarbio) electrophoresis followed by transferring into polyvinylidene difluoride membranes (Amersham Biosciences, Chicago, IL, USA). After blocking in 5% non-fat milk for 1 h at indoor temperature, the membranes were incubated overnight with primary antibodies: β-actin (bs-0061R; Bioss, Beijing, China) and HMGB1 (bs-0664R; Bioss) at 4°C and probed with horseradish peroxidase-conjugated secondary antibody (bs-0295M-HRP; Bioss) for 1 h at indoor temperature. The signals were examined using chemiluminescent substrate kit (Millipore, Bedford, MA, USA).
5
Inflammasome Activation Pathway Analysis
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Uric acid sodium was purchased from Sigma Aldrich (St. Louis, USA). Forskolin and SQ22536 were purchased from MedChemExpress (USA). Phorbol 12-myristate 13-acetate (PMA), propidium iodide (PI), 4’, 6-diamidino-2-phenylindole (DAPI), and Antifade Mounting Medium were provided by Keygen biotech (Nanjing, China). siRNA for transfection was obtained from Genepharma (Shanghai, China). Lipofectamine 2000 was derived from Invitrogen. Caspase-1 Detection Kit was supplied from ImmunoChemistry Technologies (USA). NLRP3 (bs-10021R), ASC (bs-6741R), caspase-1 (bs-10442R), and β-actin (bs-0061R) antibodies for western blot were obtained from Bioss (Beijing, China). NLRP3 (sc-34410) and ASC (sc-22514-R) primary antibodies used for immunofluorescence were obtained from Santa Cruz Biotechnology (USA). Donkey Anti-Goat IgG H&L (Alexa Fluor 488) (ab150129) and Donkey Anti-Rabbit IgG H&L (Alexa Fluor 647) (ab150075) second antibodies used for Immunofluorescence were obtained from Abcam (USA). All cell culture supplement components were obtained from Gibco (Waltham, MA, USA).
6
Western Blot Analysis of AhR Protein
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The procedure of Western blot was conducted as previously described (Wang et al., 2021 (link)). Briefly, suitably sized lung tissues were homogenized and lysed by lysis buffer (Beyotime) containing 0.5 mM protease inhibitor PMSF (Beyotime). The mixture was centrifuged at 13,000 g for 20 min at 4°C and the supernatant was the total protein. After denaturation, protein was separated by SDS-PAGE (8%–12%) and transferred to nitrocellulose membrane (Beyotime). The membrane was blocked with QuickBlock Blocking Buffer (Beyotime) for 15 min and incubated with specific primary antibodies for 4 h at room temperature. The membrane was washed at least 3 times with Tris-buffered saline with 1% Tween 20 (TBST) (Boster, Wuhan, China) and incubated with secondary goat anti-rabbit IgG (BA1056, Boster, Wuhan, China) for 45 min at room temperature. The blot was detected by enhanced chemiluminescence reagent (Boster, Wuhan, China) and analyzed by Image J (version 1.4.3.67) software. The primary antibodies used are as follows: AhR (28727-1-AP, Proteintech, Wuhan, China) and β-actin (bs-0061R, Bioss, Beijing, China).
7
Western Blot Analysis of Protein Expression
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Total protein was extracted by lysing cells with RIPA lysate I (C5000050010; Sangon Biotech, Shanghai, China). Protein concentration was measured using a BCA Protein Assay Kit (C5030210500; Sangon Biotech, China). Equal amounts of protein lysate were boiled for 5 minutes with 5× sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) loading buffer (P1040; Solarbio, Beijing, China) and separated by SDS-PAGE Preparation kit (C631100; Sangon Biotech, China) and transferred to polyvinylidene fluoride membrane (Millipore ImmobilonTM P, Billerica, MA, USA). After blocking in 5% skim milk dissolved in Trisbuffered saline (0.02 M Tris base, 0.14 M NaCl, pH 7.4) containing 0.1% Tween 20, the membrane was incubated with primary antibodies overnight at 4°C, and then with IRDye 800CW goat anti-rabbit immunoglobulin G (Li-COR Biotechnology, Lincoln, NE, USA) for 1 hour at room temperature. The primary antibodies used in this study were UCP1 (ab10983; Abcam, USA), myf5 (ab139523; Abcam, USA), PPARγ (bs4590R; Bioss, China), CEBPα (GTX100674; Gene Tex, USA), β-actin (bs0061R; Bioss, China) and glyceraldehyde-3-phosphate dehydrogenase (HC301; TransGen, Beijing, China). The blots were imaged by the Odyssey CLx Imaging System (Li-COR Biotechnology, USA) and the protein density analysis was performed using Image J (v1.45) software.
8
Antibody and Chemical Reagent Protocol
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The antibodies against Bax (50599-2-lg), Bcl-2 (60178-1-lg), and survivin (10988-1-AP) were obtained from Proteintech (Chicago, IL, USA). The anti-GADPH (bsm-0978M), HSP60 (bs-0191R) and β-actin (bs-0061R) antibodies were obtained from Bioss (Beijing, China). The AKT (# 9272S), P-AKT (# 86758S), PI3K (# 4255S), P-PI3K (# 17366S), mTOR (# 2983S), P-mTOR (# 5536S) antibodies were purchased from CST (BCG, USA). 6-SH (SS8510), Taxol (Tax, SP8020) and D, L-dithiothreitol (DTT, A285) were purchased from Solarbio (Beijing, China). 3-azido-7-hydroxycoumarin (M0135–73235) was obtained from Macklin (Shanghai, China).
9
Osteoclast Differentiation from Mouse Bone Marrow Macrophages
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Primary cultured BMMs were used to study osteoclast differentiation. Mouse bone marrow cells were isolated from 11-week-old male C57BL/6 mouse hindlimbs (femur and tibia) and incubated with M-CSF (50 ng/mL) for 96 h to obtain BMMs. The macrophage RAW264.7 cell line and human embryonic kidney 293 (HEK293) cell line were obtained from the American Type Culture Collection (Rockville, MD, USA). Cells were cultured with α-minimal essential medium (α-MEM; Gibco, Grand Island, NY, USA) containing 10% fetal bovine serum (FBS; Gibco, Grand Island, NY, USA) and 1% penicillin-streptomycin (HyClone, Thermo Scientific, MA, USA) in a 37°C incubator containing 5% CO2-enriched atmosphere. Recombinant mouse RANKL and recombinant mouse M-CSF were purchased from R&D Systems (Minneapolis, MN, USA). A TRAP stain kit was obtained from Sigma-Aldrich (NY, USA). An alizarin red stain kit was obtained from Solarbio Life Sciences (Beijing, China). Antibodies against histone 3 (bs-17422R), CD81 (bs-2489R), TSG101 (bs-1365R), Lamin A/C (bs-1839R), COL1A1 (bs-10423R), RUNX2 (bs-20003R), ALP (bsm-52252R), and β-actin (bs-0061R) were all purchased from Bioss Antibodies (Beijing, China).
10
Icariin Modulates Bone Metabolism
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Icariin (20200719) was purchased from Xi'an Yuhui Biotechnology Co. Ltd (Xi'an, China). Alkaline phosphatase (ALP) (JL26470), procollagen type I N-terminal propeptide (PINP) (JL49448), tartrate-resistant acid phosphatase isoform 5b (TRACP-5b) (JL12318), and C-telopeptide of type I collagen (CTX-I) (JL20748) enzyme-linked immunosorbent assay (ELISA) kits were purchased from Jianglai (Shanghai, China). Autophagy-related protein 7 (Atg7) (8558), Akt (9272), p-Akt (4058), Beclin1 (3495), mammalian target of rapamycin (mTOR) (2972), p-unc-51-like autophagy activating kinase 1 (ULK1) (Ser555) (5869), p-mTOR (2971), ULK1 (8054), p-ULK1 (Ser757) (14202), and AMPactivated protein kinase (AMPK) (5832) were purchased from Cell Signaling Technology (Danvers, USA). Microtubule-associated protein 1 light chain 3 (LC3) (GTX127375) was purchased from Genetex (Louis Park, USA), sequestosome 1 (p62) (ab109012) from Abcam (Cambridge, UK), β-actin (bs-0061R) from Bioss (Woburn, USA) and p-AMPK (13S4010) from Bioword (Louis Park, USA). Bicinchoninic acid (BCA) kits were purchased from Thermo Fisher Scientific (Waltham, USA) and dithiothreitol (DTT; 1 mol/L) was obtained from Sigma-Aldrich (St. Louis, USA).
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