SEM images for fish scale gelatin nanofibers without essential oil emulsions were analyzed using an FEI Inspect S50 Scanning Electron Microscope equipped with an EDX unit for elemental analysis. All samples were covered with a thin gold layer of about 20 nm to avoid the charging effect by using a sputtering Cressington 108 auto sputter coater device, equipped with a Cressington mtm 20 thickness controller. The secondary electron images were obtained at a 10 mm distance, using 10 kV acceleration voltage and magnification from 50× up to 10,000×. The nanofibers’ average thickness was reported as the mean diameter of a minimum of 50 nanofibers, using Origin’s built-in Gaussian fitting curve software, and the thickness measurement was processed using ImageJ software, version no. 1. 54d.
Inspect s50 scanning electron microscope
Manufactured by Thermo Fisher Scientific
Sourced in Netherlands
The Inspect S50 is a scanning electron microscope (SEM) designed for high-resolution imaging and analysis of samples at the nanoscale level. It features a compact and ergonomic design, allowing for easy operation and integration into various laboratory settings. The Inspect S50 offers reliable performance and consistent results, making it a versatile tool for a wide range of applications.
Automatically generated - may contain errors
Lab products found in correlation
7 protocols using inspect s50 scanning electron microscope
1
SEM Analysis of Fish Scale Gelatin Nanofibers
2
UV Inactivation and Dendritic Cell Maturation by Tannerella forsythia
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tannerella forsythia wild-type and Gtf-deficient mutants were irradiated three times with UV light using maximum UV energy settings for 1 min, each, (Ultraviolet Crosslinker CL-1000; UVP, Upland, CA, United States) for inactivation. Cell integrity after treatment was verified by scanning electron microscopy using an Inspect S50 scanning electron microscope (FEI, Eindhoven, Netherlands) (Tomek et al., 2014 (link)).
Murine and human iDCs were matured in a 48-well plate with inactivated T. forsythia wild-type, ΔTanf_01245 (ΔgtfS), ΔTanf_01290 (ΔgtfI), or ΔTanf_01305 (ΔgtfE) cells, at concentrations of 106, 107 and 108 cfu/ml. iDCs stimulated with 100 ng/ml LPS (E. coli strain O111:B4, Calbiochem, EMD Chemicals, San Diego, CA, United States) served as a positive control. Stimulated DCs were incubated at 37°C for 24 h. Supernatants were frozen for later use, DCs were harvested. DC maturation was monitored by visual and flow cytometric evaluation of typical DC morphology (LSR II flow cytometer, BD Biosciences), and expression of cell-surface markers (α-CD80, α-CD86, α-MHC-II; eBioscience) after 6 h and 24 h, respectively.
Murine and human iDCs were matured in a 48-well plate with inactivated T. forsythia wild-type, ΔTanf_01245 (ΔgtfS), ΔTanf_01290 (ΔgtfI), or ΔTanf_01305 (ΔgtfE) cells, at concentrations of 106, 107 and 108 cfu/ml. iDCs stimulated with 100 ng/ml LPS (E. coli strain O111:B4, Calbiochem, EMD Chemicals, San Diego, CA, United States) served as a positive control. Stimulated DCs were incubated at 37°C for 24 h. Supernatants were frozen for later use, DCs were harvested. DC maturation was monitored by visual and flow cytometric evaluation of typical DC morphology (LSR II flow cytometer, BD Biosciences), and expression of cell-surface markers (α-CD80, α-CD86, α-MHC-II; eBioscience) after 6 h and 24 h, respectively.
3
Scanning Electron Microscopy of T. forsythia Biofilms
4
SEM Imaging of Tannerella forsythia
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
T. forsythia wild-type and T. forsythia ∆ampG were cultivated with free MurNAc or E. coli PGN, as described above, and samples were prepared for SEM following a published protocol [38 (link)]. In short, 1 ml of each culture was harvested by centrifugation (8000 g, 7 min), cell pellets were washed with phosphate buffered saline and applied to increasing concentrations of ethanol from 25 to 100% in PBS, with incubation of 7 min and removal of ethanol by centrifugation after each step. Samples were sputter-coated with gold (EM SDC005 apparatus; Leica, Wetzlar, Germany) and imaged with an Inspect S50 scanning electron microscope (FEI, Eindhoven, The Netherlands).
5
SEM Analysis of T. forsythia Morphology
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
T. forsythia WT and ΔTf_murK::erm mutant were cultivated as described above, using MurNAc concentrations of 0.1, 0.5, 1.0, and 20.0 μg/ml, respectively. Briefly, at an OD600 of ∼0.5 (exponential growth phase) or ∼1.5 (stationary growth phase), 1 ml of bacterial culture was harvested, each, and centrifuged at 5000 g for 7 min. Cell pellets were washed twice with phosphate-buffered saline (PBS), suspended in 500 μl of ethanol (25% in PBS), incubated for 7 min at RT and centrifuged. This step was repeated using solutions of 35, 50, 60, 70, 80, 90, and 95% ethanol in PBS and finally 100% ethanol. Finally, the samples were sputter-coated with gold (EM SDC005 apparatus; Leica, Wetzlar, Germany) and imaged with an Inspect S50 scanning electron microscope (FEI, Eindhoven, Netherlands). A detailed description of sample preparation for sputter-coating and SEM is published elsewhere (Tomek et al., 2014 (link)).
6
Scanning Electron Microscopy Analysis
7
Comprehensive Physicochemical Analysis of Lignite
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The pH of RL, DL, and LSRNF was determined
in a mixture (1:25, w/v) in deionized water using a digital pH meter.
The samples were extracted to measure the EC using a digital EC meter.34 (link) The VM, A, and FC of RL and DL were measured
following the standard procedure, developed by the American Society
for Testing and Materials (ASTM).35 The
structural stability was identified by XRD. The BET surface area and
pore volume were determined by the ASAP-2020 surface area and pore
volume analyzer (Micromeritics, Norcross, GA, USA). The CEC was measured
by the method described by Takaya et al.36 (link) The surface structure of RL, DL, and urea-loaded lignite was analyzed
using an inspect S-50 scanning electron microscope (FEI, The Netherlands)
at a magnification of 50 μm and HV 30,000 kv.
in a mixture (1:25, w/v) in deionized water using a digital pH meter.
The samples were extracted to measure the EC using a digital EC meter.34 (link) The VM, A, and FC of RL and DL were measured
following the standard procedure, developed by the American Society
for Testing and Materials (ASTM).35 The
structural stability was identified by XRD. The BET surface area and
pore volume were determined by the ASAP-2020 surface area and pore
volume analyzer (Micromeritics, Norcross, GA, USA). The CEC was measured
by the method described by Takaya et al.36 (link) The surface structure of RL, DL, and urea-loaded lignite was analyzed
using an inspect S-50 scanning electron microscope (FEI, The Netherlands)
at a magnification of 50 μm and HV 30,000 kv.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!