Human tim 1 kim 1 havcr quantikine elisa kit

All analyses were performed according to standard methods [1 (link),[10] (link), [11] (link), [12] (link),14 (link),18 (link)]. Assays for NGAL (Human Lipocalin-2/NGAL Quantikine ELISA Kit, R&D Systems Inc., Minneapolis, USA), KIM-1 (Human TIM-1/KIM-1/HAVCR Quantikine ELISA Kit, R&D Systems Inc., Minneapolis, USA) and β-NAG (NAG test kit, PPR Diagnostics Ltd., London, UK) were performed using ELISA in accordance with the instructions provided by the manufacturers.

Clean-catch midstream urine specimens were collected from patients in each group at the time of admission and were collected again 48 h after admission. Patients with compromised mental status were given an indwelling catheter to obtain clean urine. After centrifugation for 5 min at 3,000 rpm, the supernatant was transferred into Eppendorf tubes and stored in a freezer at −80 °C. Urine specimens for the control group were also collected and stored at −80 °C.
Urinary KIM-1 and IL-18 levels were measured by enzyme-linked immunosorbent assay (ELISA) (Human TIM-1/KIM-1/HAVCR Quantikine ELISA Kit, R&D Systems, Minneapolis, MNUSA; Human IL-18 Platinum ELISA, eBioscience, Inc, San Diego, CA, USA). Optical density was read at 450 nm (Model 450 Microplate Reader; Bio-Rad Laboratories, Inc., Hercules, CA, USA), and the test-sample concentrations were calculated using a standard curve.