Enzygnost hiv integral 4

At the laboratory, all sera samples were tested in parallel using the 4^th-generation RDT Alere^TM HIV Combo (Alere Medical Co. Ltd) and two HIV reference ELISAs. The reference ELISAs, Innotest HIV Antigen mAb (Fujirebio Europe, Belgium) and Enzygnost HIV Integral 4 (Siemens Healthcare Diagnostics Products GmbH, Germany), were considered the gold standard serological assays for the detection of HIV p24 antigen and HIV antibodies, respectively.
All assays were performed and interpreted according to the manufacturers’ instructions and all discrepant results were repeated in duplicate. The main characteristics of all the HIV serological assays used in the present study are summarized in S4 Table.

HIV testing was done with SD Bioline HIV-1/2 3.0 rapid test (Standard Diagnostics, Inc., Korea). Non-reactive results were considered negative for HIV antibodies whereas reactive results were subsequently tested with Uni-GoldTM HIV-1/2 (Trinity Biotech Plc, Ireland). Discrepant results between the first and second rapid assays were resolved by Enzygnost HIV Integral 4 (Siemens, Germany). Presence of hepatitis B and C antibodies were tested using Murex HBsAg version 3 ELISA (Diasorin, UK) and Murex HCV ELISA (Diasorin, UK) respectively.