Acetonitrile hplc ms grade

Standards used for identification and quantification of phenolic compounds were gallic acid, syringic acid, catechin, chlorogenic acid, caffeic acid, p-coumaric acid, quercetin, rutin (quercetin-3-O-rutinoside), hyperoside (quercetin-3-O-galactoside), kaempherol, juglone (Merck, Darmstadt, Germany), ferulic acid and ellagic acid (Fluka-Honeywell, Charlotte, NC, USA). HPLC/MS grade acetonitrile and formic acid, analytical grade methanol and acetic acid were purchased from Avantor (Radnor, PA, USA). The bidistilled water was filtered through a 0.45 µm nylon membrane.

Tris was purchased from Carl Roth (Karlsruhe, Germany), isopropyl β-d-1-thiogalactopyranoside (IPTG) from Serva (Heidelberg, Germany), methacrylonitrile from Fluka (Buchs, Switzerland) and CoCl₂ and FeSO₄*7H₂O from Merck. HPLCMS grade acetonitrile was purchased from J.T.Baker/Avantor Performance Materials (Deventer, The Netherlands). All other chemicals were obtained from Sigma–Aldrich (St. Luis, MO, USA) and used without further purification.
E. coli cells were cultivated in an RS 306 shaker (Infors, Bottmingen, Switzerland), a Multitron shaker (Infors AG Bottmingen, Switzerland) and a Certomat BS-1, and the cells were harvested with an Avanti J-20 XP centrifuge (Beckman Coulter, Brea, CA, USA). Cell pellets were disrupted by a 102C converter with a Sonifier 250 (Branson, Danbury, CT, USA), and the cell-free extract was obtained by centrifugation in an Avanti J-20 XP centrifuge (Beckman Coulter). Reactions were performed on a Thermomixer comfort (Eppendorf, Hamburg, Germany). HPLC/MS analysis was carried out on an Agilent Technologies (Santa Clara, CA, USA) 1200 Series equipped with G1379B degasser, G1312B binary pump SL, G1367C HiP-ALS SL autosampler, a G1314C VWD SL UV detector, G1316B TCC SL column oven and a G1956B MSD. A positive electrospray ionization mode was used as ionization method.