Hoechst 33242

C₁₆GSH solution was loaded into the inlet ports of Iuvo™ Microchannel 5250 plates (Thermo Scientific), 1 mm wide×5.25 mm long×140 μm high. The solution was allowed to rest for 20 min before gel cross-linking was initiated by adding 1 μL of 0.1 M NaOH to the outlet port. After 1 h, gels inside the channels were washed five times with PBS by adding 3 μL to the inlet port allowing 5 min for passive pumping to occur. Gels inside the channels were sterilized with 5× P-S in PBS for 1 h, washed with DMEM (without serum) five times, and incubated at 37°C for 1 h. Schwann cells were added at a density of 2000 cells per channel to the outlet port in complete medium. Each microchannel was then checked visually to ensure cells were only present in the outlet port area. Cells were allowed to migrate across gel-filled channels for 6 days and media were replenished on days 2 and 5. On day 6, media were removed, and cells were fixed and stained with Hoechst 33242 (Sigma) and FITC-Phalloidin (Invitrogen). Images of migration were taken on a Zeiss LSM 700 laser scanning confocal microscope. ImageJ software was used to count cell nuclei that had migrated into the channel from the outlet port.

Acetonitrile, ethanol, water, paraformaldehyde, and DMSO were obtained from Merck (Darmstadt, Germany). Roswell Park Memorial Institute-1640, BSA, trypsin, streptomycin, DMEM, and penicillin were purchased from Himedia Chemicals Ltd (Mumbai, India). MTT reagent, 2′,7′-dichlo-rodihydrofluorescein diacetate (DCFH-DA) reagent, JC-1 dye, Hoechst 33242, propidium iodide (PI), RNase A, crystal violet, methylene blue, Coomassie Brilliant Blue R-250, N-acetyl-l-cysteine (NAC), SDS, Triton-X 100, Tris-base, glycine, acrylamide, bisacrylamide, ammonium persulfate, tetramethylethylenediamine, Tween-20, bicinchoninic acid reagent, and 2-mercaptoethanol were purchased from Sigma-Aldrich. All antibodies were procured from Abcam (Cam-bridge, MA, USA). Annexin V-fluorescein isothiocyanate (FITC) apoptosis kit and APO Direct kit were obtained from BD Biosciences (San Jose, CA, USA). Caspase-3 substrate (Ac-DEVD-AMC), caspase-8 substrate (Ac-IETD-AMC), and caspase-9 substrate (Ac-LEHD-AMC) were obtained from Cayman Chemicals. Polyvinylidene difluoride membrane was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Matrigel invasion chambers with 8 µm pore size inserts were obtained from BD Biosciences. The reference standards used, that is, coronarin-D (purity >99%) and coronarin-D methyl ether (purity >99%), were purchased from Wuhan ChemFaces Biochemical Co., Ltd (Wuhan, China).